Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,559,680	G→A	G77G (GGC→GGT)	ddpC ←	D,D‑dipeptide ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,559,680	0	G	A	100.0%	176.2 / NA	55	G77G (GGC→GGT)	ddpC	D,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

GCACACCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACG  >  NC_000913/1559550‑1559812                                                                                                                                   |                                                                                                                                     gCACACCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCa                                                                                                                              <  2:145133/139‑1 (MQ=255)    cacCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTg                                                                                                                           <  2:303111/139‑1 (MQ=255)     acCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTgc                                                                                                                          >  2:297574/1‑139 (MQ=255)        ggATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGcgcc                                                                                                                       >  1:716700/1‑139 (MQ=255)          aTAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGcgccgc                                                                                                                     >  2:541469/1‑139 (MQ=255)                aTCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAgg                                                                                                               <  1:297574/139‑1 (MQ=255)                   cGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAg                                                                                                                   <  1:1433510/132‑1 (MQ=255)                   cGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAg                                                                                                                   >  2:1433510/1‑132 (MQ=255)                        aGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCaaaa                                                                                                       >  1:443602/1‑139 (MQ=255)                        aGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACAAGTGCGCCGCAGAAGGCGGCaaaa                                                                                                       >  1:856960/1‑139 (MQ=255)                            aaCCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcg                                                                                                   >  1:6480/1‑139 (MQ=255)                            aaCCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcg                                                                                                   >  1:1140193/1‑139 (MQ=255)                            aaCCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcg                                                                                                   >  2:791833/1‑139 (MQ=255)                            aaCCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcg                                                                                                   >  2:1481264/1‑139 (MQ=255)                              ccAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcggg                                                                                                 <  1:1481264/139‑1 (MQ=255)                              ccAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcggg                                                                                                 <  2:856960/139‑1 (MQ=255)                              ccAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcggg                                                                                                 <  2:1140193/139‑1 (MQ=255)                               cAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcgggc                                                                                                <  1:875444/139‑1 (MQ=255)                                          gCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCaa                                                                                     <  1:1035743/139‑1 (MQ=255)                                          gCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCaa                                                                                     <  2:6480/139‑1 (MQ=255)                                           cAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAAt                                                                                    <  2:121642/139‑1 (MQ=255)                                            aaTGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATg                                                                                   >  1:770509/1‑139 (MQ=255)                                                          cTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcg                                                                                                   >  2:227528/1‑109 (MQ=255)                                                          cTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAggcg                                                                                                   <  1:227528/109‑1 (MQ=255)                                                             aTCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCaa                                                                                     <  2:408800/120‑1 (MQ=255)                                                             aTCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCaa                                                                                     >  1:408800/1‑120 (MQ=255)                                                             aTCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGt                                                                  >  1:1325656/1‑139 (MQ=255)                                                               ccGGCGAGAATTGATTGCTGACTGCCGNCCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAAACAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCg                                                                >  2:1512858/1‑139 (MQ=255)                                                                  gCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCgcca                                                             >  1:124035/1‑139 (MQ=255)                                                                      gaATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCgccagcca                                                         <  1:541469/139‑1 (MQ=255)                                                                        attgattgCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACg                                                       <  2:943505/139‑1 (MQ=255)                                                                                      gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGa                                                                                                                  >  2:1103057/1‑66 (MQ=255)                                                                                      gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGa                                                                                                                  <  1:1103057/66‑1 (MQ=255)                                                                                      gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                         <  2:124035/139‑1 (MQ=255)                                                                                      gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                         <  1:299863/139‑1 (MQ=255)                                                                                      gCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatcatc                                         <  1:1201601/139‑1 (MQ=255)                                                                                           ccAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCaa                                    <  2:716700/139‑1 (MQ=255)                                                                                            cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                                   <  2:1325656/139‑1 (MQ=255)                                                                                            cAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                                   <  1:791833/139‑1 (MQ=255)                                                                                                 cgcgGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAg                                 <  2:706819/136‑1 (MQ=255)                                                                                                 cgcgGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAg                                 >  1:706819/1‑136 (MQ=255)                                                                                                 cgcgGCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCAt                              <  2:770509/139‑1 (MQ=255)                                                                                                     gCTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAAt                          <  2:1134327/139‑1 (MQ=255)                                                                                                      cTAAACAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATa                         >  1:117445/1‑139 (MQ=255)                                                                                                         aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAAt                                                                                    <  1:928500/77‑1 (MQ=255)                                                                                                         aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAAt                                                                                    >  2:928500/1‑77 (MQ=255)                                                                                                         aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                                   <  2:1465141/126‑1 (MQ=255)                                                                                                         aaCAGATCGCGTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAAt                                   >  1:1465141/1‑126 (MQ=255)                                                                                                                cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCAt                              <  1:483756/124‑1 (MQ=255)                                                                                                                cgcgTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCAt                              >  2:483756/1‑124 (MQ=255)                                                                                                                   gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCg                                                                                <  1:1102808/71‑1 (MQ=255)                                                                                                                   gTCCCACTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCg                                                                                >  2:1102808/1‑71 (MQ=255)                                                                                                                        aCTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatc                                            >  2:284741/1‑102 (MQ=255)                                                                                                                        aCTTCATCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAatc                                            <  1:284741/102‑1 (MQ=255)                                                                                                                             aTCGGTACCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACg  >  2:107585/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                     GCACACCGGATAGACATCCGAGTAGCGAACCAATCATACCCGCAATGGCAACCACGACTAATCCGGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACG  >  NC_000913/1559550‑1559812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑