Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,614,789	C→T	intergenic (‑86/‑15)	sad ← / → yneJ	succinate semialdehyde dehydrogenase, NAD(P)+‑dependent/putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,614,789	0	C	T	100.0%	163.0 / NA	52	intergenic (‑86/‑15)	sad/yneJ	succinate semialdehyde dehydrogenase, NAD(P)+‑dependent/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/22);  total (30/22)

GGCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCT  >  NC_000913/1614659‑1614919                                                                                                                                   |                                                                                                                                   ggCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAAGATTAAGCCACTTATTCATGAATCgag                                                                                                                            <  1:829905/139‑1 (MQ=255)   cAGGATTTATCGAAATTGCTTGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          <  2:1004778/139‑1 (MQ=255)   cAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          <  1:1436092/139‑1 (MQ=255)        tttATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTa                                                                                                                     <  1:891805/139‑1 (MQ=255)                aaTTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTg                                                                                                             >  1:1301129/1‑139 (MQ=255)                 aTTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGa                                                                                                            <  2:649030/139‑1 (MQ=255)                                    tAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCaa                                                                                         <  2:602307/139‑1 (MQ=255)                                             tCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGc                                                                                >  2:758095/1‑139 (MQ=255)                                                     tctcCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTg                                                                        >  2:579166/1‑139 (MQ=255)                                                               gAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAAt                                                                                                                                >  1:114093/1‑73 (MQ=255)                                                               gAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAAt                                                                                                                                <  2:114093/73‑1 (MQ=255)                                                                      ggTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCt                                                       >  2:1362117/1‑139 (MQ=255)                                                                            aaGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          >  1:148790/1‑66 (MQ=255)                                                                            aaGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGaa                                                                                                                          <  2:148790/66‑1 (MQ=255)                                                                            aaGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTgcagca                                                 >  2:563100/1‑139 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTa                                                                                                                     <  2:1383585/65‑1 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTa                                                                                                                     >  1:1383585/1‑65 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcagc                                                                   <  2:1146644/115‑1 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcagc                                                                   >  1:1146644/1‑115 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                           >  1:790370/1‑139 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                           >  2:460050/1‑139 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                           >  2:25520/1‑139 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTg                                           >  2:1114146/1‑139 (MQ=255)                                                                                  cGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACACAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGGTGCAGCAAAAGTg                                           >  2:713446/1‑139 (MQ=255)                                                                                    cAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGgag                                                                                                 >  2:93588/1‑83 (MQ=255)                                                                                    cAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGgag                                                                                                 <  1:93588/83‑1 (MQ=255)                                                                                     aGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCAt                                        <  1:579166/139‑1 (MQ=255)                                                                                      gATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcagca                                                                  >  2:162641/1‑112 (MQ=255)                                                                                      gATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGgcagca                                                                  <  1:162641/112‑1 (MQ=255)                                                                                            ctctTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGc                                 >  1:251549/1‑139 (MQ=255)                                                                                             tctTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                       <  1:117259/100‑1 (MQ=255)                                                                                             tctTGCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                       >  2:117259/1‑100 (MQ=255)                                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgag                                                                                                                            <  1:464892/43‑1 (MQ=255)                                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCgag                                                                                                                            >  2:464892/1‑43 (MQ=255)                                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTTGCAGCATAACCCAGGc                                                        >  2:238460/1‑111 (MQ=255)                                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGc                                                        <  1:238460/111‑1 (MQ=255)                                                                                                 gCTTTAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCg                            >  2:355207/1‑139 (MQ=255)                                                                                                    ttAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGAc                                                                                                           <  2:1367874/57‑1 (MQ=255)                                                                                                    ttAAAATGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGAc                                                                                                           >  1:1367874/1‑57 (MQ=255)                                                                                                      aaaaTGAATAATATTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAAtct                       <  2:790370/139‑1 (MQ=255)                                                                                                                atatTAAGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGt             <  1:1415818/139‑1 (MQ=255)                                                                                                                      aGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                       >  1:58008/1‑75 (MQ=255)                                                                                                                      aGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTgg                                                                       <  2:58008/75‑1 (MQ=255)                                                                                                                      aGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgcgc       <  1:355207/139‑1 (MQ=255)                                                                                                                      aGCCACTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTgcgc       >  1:1167810/1‑139 (MQ=255)                                                                                                                           cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt  >  2:391356/1‑139 (MQ=255)                                                                                                                           cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt  >  2:150469/1‑139 (MQ=255)                                                                                                                           cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt  >  1:1202472/1‑139 (MQ=255)                                                                                                                           cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt  >  1:1450517/1‑139 (MQ=255)                                                                                                                           cTTATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCt  >  2:1323157/1‑139 (MQ=255)                                                                                                                             tATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCt                                                                         >  1:1320683/1‑66 (MQ=255)                                                                                                                             tATTCATGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCt                                                                         <  2:1320683/66‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   GGCAGGATTTATCGAAATTGCATGAGTTGCCGGAGTAATGGTCATCGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTTATTCACGAATCGAGAATGCTATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATAACCCAGGCTGCAGCAAAAGTGCATCGCGTGCCGTCGAATCTCACTACCCGTCTGCGCCAGCT  >  NC_000913/1614659‑1614919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑