Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,957	G→A	*128* (TAG→TAA)	marA →	multiple antibiotic resistance transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,957	0	G	A	100.0%	159.4 / NA	50	*128* (TAG→TAA)	marA	multiple antibiotic resistance transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/23);  total (27/23)

GAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCAATGT  >  NC_000913/1619832‑1620092                                                                                                                              |                                                                                                                                        gAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGAc                                                                                                                            >  1:199834/1‑139 (MQ=255)                    cccGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCa                                                                                                         >  1:54901/1‑139 (MQ=255)                       gAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGa                                                                                                      <  1:710842/139‑1 (MQ=255)                             tCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGaaaa                                                                                                                                  >  2:229469/1‑105 (MQ=255)                             tCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGaaaa                                                                                                                                  <  1:229469/105‑1 (MQ=255)                                     tACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATcc                                                                                        <  1:535878/139‑1 (MQ=255)                                                tCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagcagc                                                                             <  2:1377891/139‑1 (MQ=255)                                                 ccgccgCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCt                                                                            >  2:1156990/1‑139 (MQ=255)                                                 ccgccgCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCt                                                                            >  2:1209127/1‑139 (MQ=255)                                                     cgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcgc                                                                        <  1:1209127/139‑1 (MQ=255)                                                                     gACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCcgc                                                        >  2:1249243/1‑139 (MQ=255)                                                                       ccAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCAt                                                                                          <  1:382105/103‑1 (MQ=255)                                                                       ccAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCAt                                                                                          >  2:382105/1‑103 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACt                                                                                                                   <  1:527164/67‑1 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACt                                                                                                                   >  2:527164/1‑67 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCa                                                                                                             >  1:52329/1‑73 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCa                                                                                                             <  2:52329/73‑1 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGaaa                                                                                                    >  2:1388619/1‑82 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGaaa                                                                                                    <  1:1388619/82‑1 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCg                                           >  1:1523743/1‑139 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCg                                           >  2:1439379/1‑139 (MQ=255)                                                                                  ggCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCg                                           >  2:1279966/1‑139 (MQ=255)                                                                                       aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACaa                                                                                                                          >  1:1044731/1‑55 (MQ=255)                                                                                       aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACaa                                                                                                                          <  2:1044731/55‑1 (MQ=255)                                                                                       aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATg                                                                                                       >  2:961501/1‑74 (MQ=255)                                                                                       aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATg                                                                                                       <  1:961501/74‑1 (MQ=255)                                                                                       aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACtt                                                                                              <  1:872701/83‑1 (MQ=255)                                                                                       aTCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACtt                                                                                              >  2:872701/1‑83 (MQ=255)                                                                                                    cATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCa                         <  1:1156990/139‑1 (MQ=255)                                                                                                    cATCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCa                         <  2:1523743/139‑1 (MQ=255)                                                                                                     aTCCATTAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAg                        <  2:54901/139‑1 (MQ=255)                                                                                                          ttAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATag                                                                                 <  1:681989/77‑1 (MQ=255)                                                                                                          ttAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATag                                                                                 >  2:681989/1‑77 (MQ=255)                                                                                                          ttAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACCAACCACGCAGCCAgttgtt                   >  1:1160788/1‑139 (MQ=255)                                                                                                          ttAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAgttgtt                   >  1:912315/1‑139 (MQ=255)                                                                                                           tAAATCATTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTCTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTa                  <  2:1160788/139‑1 (MQ=255)                                                                                                                 aTTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAg                        <  1:812560/127‑1 (MQ=255)                                                                                                                 aTTACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAg                        >  2:812560/1‑127 (MQ=255)                                                                                                                  ttACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCAt                                                                                                        >  1:712317/1‑46 (MQ=255)                                                                                                                  ttACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCAt                                                                                                        <  2:712317/46‑1 (MQ=255)                                                                                                                  ttACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTtg           <  1:1439379/139‑1 (MQ=255)                                                                                                                   tACAACAGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTtgt          >  2:1422289/1‑139 (MQ=255)                                                                                                                    acaacaGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGtt                                              <  2:1432298/102‑1 (MQ=255)                                                                                                                    acaacaGCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGtt                                              >  1:1432298/1‑102 (MQ=255)                                                                                                                       acaGCTAATTGAAAGCGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgc                                                                          >  2:1256012/1‑71 (MQ=255)                                                                                                                       acaGCTAATTGAAAGCGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgc                                                                          <  1:1256012/71‑1 (MQ=255)                                                                                                                          gCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGcc       >  2:457006/1‑135 (MQ=255)                                                                                                                          gCTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGcc       <  1:457006/135‑1 (MQ=255)                                                                                                                           cTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCAc                                >  1:696156/1‑109 (MQ=255)                                                                                                                           cTAATTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCAATGt  >  1:1282407/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                        GAGTCGCAACAAACTCTGACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTCCGCGCAGGGCGTTGCGGAACAAACCACGCAGCCAGTTGTTACTTCTTGTGCCAATGT  >  NC_000913/1619832‑1620092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑