| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 152,036 | C→T | V66I (GTA→ATA) | yadL ← | putative fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 152,036 | 0 | C | T | 98.3% | 180.0 / ‑6.6 | 58 | V66I (GTA→ATA) | yadL | putative fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (28/29); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAC‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTAC > NC_000913/151905‑152162 | aGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCa < 2:488746/139‑1 (MQ=255) ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATc > 1:429404/1‑139 (MQ=255) ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATc < 2:1458177/139‑1 (MQ=255) ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATc > 2:1418838/1‑139 (MQ=255) tgtgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa < 1:1418838/139‑1 (MQ=255) tgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAAt > 2:1224008/1‑139 (MQ=255) cGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCt > 1:1084124/1‑139 (MQ=255) cTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATAT‑‑ATCACCAAAATCAACAACAGAAATATCCt > 2:1413298/1‑139 (MQ=255) tAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCagag < 1:52284/139‑1 (MQ=255) aaTTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATATATATCACCAAAATCAACAACAGAAATATCCTGATCaga < 1:1413298/139‑1 (MQ=255) tttGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTAc > 2:1347067/1‑139 (MQ=255) cTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGt < 1:95946/139‑1 (MQ=255) cTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGt < 1:677423/139‑1 (MQ=255) gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa > 2:610768/1‑97 (MQ=255) gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa < 1:610768/97‑1 (MQ=255) aCCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAccc < 1:1224008/139‑1 (MQ=255) cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa > 1:1318296/1‑89 (MQ=255) cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa < 2:1318296/89‑1 (MQ=255) cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTc > 1:1378931/1‑139 (MQ=255) acaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCt > 2:1119949/1‑139 (MQ=255) aGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc < 2:1461017/139‑1 (MQ=255) aGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc < 1:1119949/139‑1 (MQ=255) tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCagag > 2:911077/1‑99 (MQ=255) tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCagag < 1:911077/99‑1 (MQ=255) tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCAttt > 2:1399921/1‑133 (MQ=255) tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCAttt < 1:1399921/133‑1 (MQ=255) tttGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa < 1:554864/82‑1 (MQ=255) tttGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa > 2:554864/1‑82 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa < 2:364297/74‑1 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa > 1:364297/1‑74 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCagag > 2:34921/1‑89 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCagag < 1:34921/89‑1 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa < 1:1383324/116‑1 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa > 2:1383324/1‑116 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc > 1:1040805/1‑139 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc > 1:1228056/1‑139 (MQ=255) tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc > 1:1273923/1‑139 (MQ=255) gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt < 1:1347067/139‑1 (MQ=255) gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt < 1:485887/139‑1 (MQ=255) gTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt < 2:429404/139‑1 (MQ=255) aaTGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAttt > 2:7265/1‑87 (MQ=255) aaTGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAttt < 1:7265/87‑1 (MQ=255) aaTGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTc < 1:233274/139‑1 (MQ=255) ttGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCa > 2:1272899/1‑66 (MQ=255) ttGGTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCa < 1:1272899/66‑1 (MQ=255) ggTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAccc > 1:852311/1‑97 (MQ=255) ggTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAccc < 2:852311/97‑1 (MQ=255) ggTCTTGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGtt > 1:47188/1‑139 (MQ=255) ttGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATc > 2:675047/1‑38 (MQ=255) ttGGCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATc < 1:675047/38‑1 (MQ=255) ggCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa < 1:11654/46‑1 (MQ=255) ggCATTGATTTCAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGaa > 2:11654/1‑46 (MQ=255) aTTTCAGAA‑‑ATATATAC‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagaga < 2:1040805/139‑1 (MQ=255) cAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGc > 1:729695/1‑67 (MQ=255) cAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGc < 2:729695/67‑1 (MQ=255) cAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc > 1:1485346/1‑88 (MQ=255) cAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc < 2:1485346/88‑1 (MQ=255) cAGAA‑‑ATATATAT‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTAc > 2:1452373/1‑139 (MQ=255) | AGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAA‑‑ATATATAC‑‑ATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTAC > NC_000913/151905‑152162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |