Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,649,041 G→A *73* (TAG→TAA)  ydfC → uncharacterized protein, Qin prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,649,0410GA100.0% 135.2 / NA 42*73* (TAG→TAA) ydfCuncharacterized protein, Qin prophage
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

CGCAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTC  >  NC_000913/1648908‑1649168
                                                                                                                                     |                                                                                                                               
cgcAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCt                                                                                                                            >  1:178698/1‑139 (MQ=255)
        cTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATcaaca                                                                                                                    <  1:780399/139‑1 (MQ=255)
              tGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTa                                                                                                              <  2:178698/139‑1 (MQ=255)
               gggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAAACAACAAAGCTaa                                                                                                             >  2:514160/1‑139 (MQ=255)
                ggCTAATGAAGCCAGGGCTAAGCAGGCTG‑AAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAgg                                                                                                           >  1:549819/1‑139 (MQ=255)
                   tAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGtt                                                                                                         <  1:396096/139‑1 (MQ=255)
                           ccAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTa                                                                                                 >  2:1047313/1‑139 (MQ=255)
                                    aGCAGGCTG‑AAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTtctc                                                                                       <  2:549819/139‑1 (MQ=255)
                                     gCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATcaaca                                                                                                                    <  2:1002656/110‑1 (MQ=255)
                                     gCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATcaaca                                                                                                                    >  1:1002656/1‑110 (MQ=255)
                                               aaTCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCt                                                                                                               >  1:274109/1‑105 (MQ=255)
                                               aaTCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCt                                                                                                               <  2:274109/105‑1 (MQ=255)
                                                    gTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGtt                                                                                                         >  2:1021239/1‑106 (MQ=255)
                                                    gTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGtt                                                                                                         <  1:1021239/106‑1 (MQ=255)
                                                             ggAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGtgcgtgcg                                                               <  1:1329141/139‑1 (MQ=255)
                                                                    gATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGaca                                                        <  1:1106524/139‑1 (MQ=255)
                                                                         cACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTg                                                                          >  1:211165/1‑116 (MQ=255)
                                                                         cACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTg                                                                          <  2:211165/116‑1 (MQ=255)
                                                                              aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGtt                                                                                                         >  2:550425/1‑80 (MQ=255)
                                                                              aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGtt                                                                                                         <  1:550425/80‑1 (MQ=255)
                                                                              aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCa                                              >  1:522124/1‑139 (MQ=255)
                                                                              aTCTGGAAAAAGCAGCGGCATTAAGCATTACCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGG‑TAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTTCGTGCGCCGGac                                                         >  2:342813/1‑127 (MQ=255)
                                                                              aTCTGGAAAAAGCAGCGGCATTAAGCATTACCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAA‑GTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGac                                                         <  1:342813/127‑1 (MQ=255)
                                                                               tCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                             <  2:498310/139‑1 (MQ=255)
                                                                               tCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                             >  1:312827/1‑139 (MQ=255)
                                                                                        agcagcGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAAACAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTg                                    <  1:514160/139‑1 (MQ=255)
                                                                                                  ttAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATc                                            >  2:717101/1‑121 (MQ=255)
                                                                                                  ttAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATc                                            <  1:717101/121‑1 (MQ=255)
                                                                                                    aaGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGa                        <  1:1047313/139‑1 (MQ=255)
                                                                                                          tGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAgtgcgtg                                                                 >  2:210242/1‑92 (MQ=255)
                                                                                                          tGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAgtgcgtg                                                                 <  1:210242/92‑1 (MQ=255)
                                                                                                                aaTGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGt                                                                                >  2:200762/1‑71 (MQ=255)
                                                                                                                aaTGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGt                                                                                <  1:200762/71‑1 (MQ=255)
                                                                                                                 aTGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAAtt                                                                                                  >  1:67046/1‑52 (MQ=255)
                                                                                                                 aTGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAAtt                                                                                                  <  2:67046/52‑1 (MQ=255)
                                                                                                                 aTGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTccc           >  1:424838/1‑139 (MQ=255)
                                                                                                                 aTGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTccc           <  2:522124/139‑1 (MQ=255)
                                                                                                                    aTTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGt                                                                                <  2:1186884/67‑1 (MQ=255)
                                                                                                                    aTTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGt                                                                                >  1:1186884/1‑67 (MQ=255)
                                                                                                                          tACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACgg                                                                                  >  1:485815/1‑59 (MQ=255)
                                                                                                                          tACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACgg                                                                                  <  2:485815/59‑1 (MQ=255)
                                                                                                                          tACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTc  >  1:70254/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                               
CGCAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTC  >  NC_000913/1648908‑1649168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑