Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,656,744	G→A	*187* (TAG→TAA)	speG →	spermidine N(1)‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,656,744	0	G	A	100.0%	215.8 / NA	68	*187* (TAG→TAA)	speG	spermidine N(1)‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/35);  total (33/35)

GAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTA  >  NC_000913/1656610‑1656874                                                                                                                                       |                                                                                                                                   gAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACAtt                                                                                                                                >  2:675531/1‑139 (MQ=255)              tGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcgat                                                                                                                   <  1:675531/139‑1 (MQ=255)               gCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcga                                                                                                                    <  2:1189742/137‑1 (MQ=255)               gCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcga                                                                                                                    >  1:1189742/1‑137 (MQ=255)                cACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcgatcg                                                                                                                 >  1:531295/1‑139 (MQ=255)                                aTGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTa                                                                                                 >  2:1205281/1‑139 (MQ=255)                                   gTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  1:1008695/129‑1 (MQ=255)                                   gTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  2:1008695/1‑129 (MQ=255)                                   gTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  1:592925/129‑1 (MQ=255)                                   gTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  2:592925/1‑129 (MQ=255)                                       atatCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTaa                                                                                                >  2:1236892/1‑133 (MQ=255)                                       atatCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTaa                                                                                                <  1:1236892/133‑1 (MQ=255)                                                  ccATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcgatc                                                                                                                  <  1:86783/104‑1 (MQ=255)                                                  ccATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAatcgatc                                                                                                                  >  2:86783/1‑104 (MQ=255)                                                    aTTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGtt                                                                             >  1:1114665/1‑139 (MQ=255)                                                     ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcggc                                                                                  >  1:990319/1‑133 (MQ=255)                                                     ttCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcggc                                                                                  <  2:990319/133‑1 (MQ=255)                                                            gtgtATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCt                                                                     >  1:55515/1‑139 (MQ=255)                                                                atatTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTtcat                                                                 <  2:428286/139‑1 (MQ=255)                                                                    tCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATcaac                                                             >  1:332242/1‑139 (MQ=255)                                                                    tCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATcaac                                                             >  1:739931/1‑139 (MQ=255)                                                                      cAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  1:381895/1‑94 (MQ=255)                                                                      cAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  2:381895/94‑1 (MQ=255)                                                                       aGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAAt                                                          <  2:332242/139‑1 (MQ=255)                                                                        gCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACAtt                                                                                                                                >  2:1094665/1‑68 (MQ=255)                                                                        gCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACAtt                                                                                                                                <  1:1094665/68‑1 (MQ=255)                                                                         cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcggcg                                                                                 <  1:301312/114‑1 (MQ=255)                                                                         cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcggcg                                                                                 >  2:301312/1‑114 (MQ=255)                                                                         cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGa                                                        >  1:958674/1‑139 (MQ=255)                                                                         cATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGa                                                        >  2:553772/1‑139 (MQ=255)                                                                                 tCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGa                                                <  1:553772/139‑1 (MQ=255)                                                                                 tCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGa                                                <  2:1349231/139‑1 (MQ=255)                                                                                    ggCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  1:541892/80‑1 (MQ=255)                                                                                    ggCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  2:541892/1‑80 (MQ=255)                                                                                        gagCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCa                                         <  1:198474/139‑1 (MQ=255)                                                                                         agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACa                                                                                                                                  <  1:635399/49‑1 (MQ=255)                                                                                         agCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCAc                                        >  1:1418085/1‑139 (MQ=255)                                                                                              aaaaCACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGAt                                   <  2:1195722/139‑1 (MQ=255)                                                                                                aaCACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcg                                                                                    <  1:176058/88‑1 (MQ=255)                                                                                                aaCACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGAcg                                                                                    >  2:176058/1‑88 (MQ=255)                                                                                                 acacCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAg                                >  1:1074860/1‑139 (MQ=255)                                                                                                      gggTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           <  2:55515/139‑1 (MQ=255)                                                                                                      gggTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGa                           <  2:739931/139‑1 (MQ=255)                                                                                                        gTCAGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACt                         <  2:1074860/139‑1 (MQ=255)                                                                                                           aGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTc                      <  2:1418085/139‑1 (MQ=255)                                                                                                           aGACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTc                      <  2:1498948/139‑1 (MQ=255)                                                                                                            gACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGt                             >  1:517072/1‑131 (MQ=255)                                                                                                            gACTCTCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGt                             <  2:517072/131‑1 (MQ=255)                                                                                                              ctctCCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCata                   >  1:780763/1‑139 (MQ=255)                                                                                                                 tcCTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTc                >  2:611370/1‑139 (MQ=255)                                                                                                                  ccTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTAt                                                                                                              >  2:733015/1‑44 (MQ=255)                                                                                                                  ccTGAAGCCGACCGCACAATAACATTAATAATAATCGATCGTAt                                                                                                              <  1:733015/44‑1 (MQ=255)                                                                                                                       aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAg            <  2:32392/137‑1 (MQ=255)                                                                                                                       aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAg            >  1:32392/1‑137 (MQ=255)                                                                                                                       aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGct          <  2:531295/139‑1 (MQ=255)                                                                                                                       aGCCGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGct          <  2:780763/139‑1 (MQ=255)                                                                                                                         ccGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  2:560935/1‑43 (MQ=255)                                                                                                                         ccGACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  1:560935/43‑1 (MQ=255)                                                                                                                           gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        <  2:383338/41‑1 (MQ=255)                                                                                                                           gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGa                                                                                                        >  1:383338/1‑41 (MQ=255)                                                                                                                           gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTcac              >  2:644081/1‑131 (MQ=255)                                                                                                                           gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTcac              <  1:644081/131‑1 (MQ=255)                                                                                                                           gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGt      >  2:204145/1‑139 (MQ=255)                                                                                                                           gACCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGt      <  1:1129075/139‑1 (MQ=255)                                                                                                                            aCCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGtttt                                                                           <  2:340469/69‑1 (MQ=255)                                                                                                                            aCCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGtttt                                                                           >  1:340469/1‑69 (MQ=255)                                                                                                                            aCCGCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATGAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGtt     >  2:1116105/1‑139 (MQ=255)                                                                                                                               gCACAATAACATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTa  <  2:958674/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   GAAGGTGAATTGATGCACGAGTTCTTTATTAATGGTCAATATCGTAATGCCATTCGCATGTGTATATTCCAGCATCAGTATCTGGCAGAGCACAAAACACCGGGTCAGACTCTCCTGAAGCCGACCGCACAATAGCATTAATAATAATCGATCGTATTTTTGATGGTGTAAACCCGTTCGACGGCGGGTTTTACTCCTTCATCAACAATGATTAGCTGACAGTCCACCGGATTAGCGTGACTGTCATATTCACAGCTCTGTTTTA  >  NC_000913/1656610‑1656874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑