Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,673,624 C→T intergenic (‑123/‑289) mdtJ ← / → tqsA multidrug efflux system transporter/pheromone AI‑2 transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,673,6240CT100.0% 236.2 / NA 74intergenic (‑123/‑289)mdtJ/tqsAmultidrug efflux system transporter/pheromone AI‑2 transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/39);  total (35/39)

ATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAA  >  NC_000913/1673490‑1673757
                                                                                                                                      |                                                                                                                                     
ataaataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGtg                                                                                                                                   <  1:261672/139‑1 (MQ=255)
ataaataTACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGtg                                                                                                                                   <  2:280962/139‑1 (MQ=255)
              tCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGa                                                                                                                     >  1:225360/1‑139 (MQ=255)
                cTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGc                                                                                                                   >  1:1308812/1‑139 (MQ=255)
                  tctcCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCaa                                                                                                                 >  2:635022/1‑139 (MQ=255)
                   ctcCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAg                                                                                                                >  1:1526935/1‑139 (MQ=255)
                   ctcCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAg                                                                                                                >  2:296145/1‑139 (MQ=255)
                    tcCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGa                                                                                                               <  2:350719/139‑1 (MQ=255)
                     ccTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGAt                                                                                                              <  1:1189286/139‑1 (MQ=255)
                        gCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGtg                                                                                                                                   >  2:1155001/1‑115 (MQ=255)
                        gCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGtg                                                                                                                                   <  1:1155001/115‑1 (MQ=255)
                        gCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGt                                                                                                           >  2:1122734/1‑139 (MQ=255)
                        gCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGt                                                                                                           >  2:915343/1‑139 (MQ=255)
                        gCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGt                                                                                                           >  2:1050495/1‑139 (MQ=255)
                           agagAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGa                                                                                                        <  1:289619/139‑1 (MQ=255)
                           agagAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGa                                                                                                        <  1:1050495/139‑1 (MQ=255)
                            gagaATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGAt                                                                                                       <  1:296145/139‑1 (MQ=255)
                            gagaATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGAt                                                                                                       <  1:915343/139‑1 (MQ=255)
                            gagaATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGAt                                                                                                       <  2:1308812/139‑1 (MQ=255)
                                attattTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAgg                                                                                                   >  2:1271788/1‑139 (MQ=255)
                                         aTTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAg                                                                                                                    >  2:1524364/1‑113 (MQ=255)
                                         aTTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAg                                                                                                                    <  1:1524364/113‑1 (MQ=255)
                                           tttCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATgg                                                                                        >  2:236748/1‑139 (MQ=255)
                                            ttCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATggg                                                                                       >  2:1156603/1‑139 (MQ=255)
                                            ttCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATggg                                                                                       >  1:233442/1‑139 (MQ=255)
                                              cGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGa                                                                                           >  2:43207/1‑133 (MQ=255)
                                              cGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGa                                                                                           <  1:43207/133‑1 (MQ=255)
                                               gCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTaa                                                                                    <  2:233442/139‑1 (MQ=255)
                                                 ttAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAAt                                                                                  <  1:670431/139‑1 (MQ=255)
                                                   aaTTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGt                                                                                <  1:635022/139‑1 (MQ=255)
                                                    atgcAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTa                                                                               <  2:1020118/136‑1 (MQ=255)
                                                    aTTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTa                                                                               <  1:1122734/139‑1 (MQ=255)
                                                        aGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCa                                                                                                                  <  2:39107/100‑1 (MQ=255)
                                                        aGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCa                                                                                                                  >  1:39107/1‑100 (MQ=255)
                                                           gAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGc                                                                                                                   <  2:976844/96‑1 (MQ=255)
                                                           gAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCACAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCAACTACGAGc                                                                                                                   >  1:976844/1‑96 (MQ=255)
                                                             aaCCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGa                                                                                                     >  1:541516/1‑108 (MQ=255)
                                                             aaCCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGa                                                                                                     <  2:541516/108‑1 (MQ=255)
                                                               ccAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTa                                                                                                 >  1:220590/1‑110 (MQ=255)
                                                               ccAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTa                                                                                                 <  2:220590/110‑1 (MQ=255)
                                                                       aagtGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACa                                                            >  2:121361/1‑139 (MQ=255)
                                                                          tGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCt                                                                                                                         <  1:1409486/75‑1 (MQ=255)
                                                                          tGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCt                                                                                                                         >  2:1409486/1‑75 (MQ=255)
                                                                           ggTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAAtt                                                        >  1:1359937/1‑139 (MQ=255)
                                                                           ggTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAAtt                                                        <  2:1359937/139‑1 (MQ=255)
                                                                            gTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTg                                                       >  2:181093/1‑139 (MQ=255)
                                                                              cAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTc                                                     <  1:181093/139‑1 (MQ=255)
                                                                               aaGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGt                                                                                                  >  2:946010/1‑93 (MQ=255)
                                                                               aaGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGt                                                                                                  <  1:946010/93‑1 (MQ=255)
                                                                               aaGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGt                                                                                      <  2:1536293/105‑1 (MQ=255)
                                                                               aaGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGt                                                                                      >  1:1536293/1‑105 (MQ=255)
                                                                                aGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCa                                                   <  2:147522/139‑1 (MQ=255)
                                                                                 gCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCat                                                  <  1:1271788/139‑1 (MQ=255)
                                                                                  ccccGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCata                                                 <  1:121361/139‑1 (MQ=255)
                                                                                   cccGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCataa                                                <  1:1156603/139‑1 (MQ=255)
                                                                                    ccGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCataat                                               >  2:931579/1‑139 (MQ=255)
                                                                                      gcgcATAGGGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCAGAGCAAATTGTCCATAacat                                             <  2:225360/139‑1 (MQ=255)
                                                                                       cgcATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTgg                                                                                                         <  1:1336477/78‑1 (MQ=255)
                                                                                       cgcATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTgg                                                                                                         >  2:1336477/1‑78 (MQ=255)
                                                                                       cgcATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATAtt                                            >  1:1138470/1‑139 (MQ=255)
                                                                                            aGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCt                                                                                                                         <  2:1211331/57‑1 (MQ=255)
                                                                                            aGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCt                                                                                                                         >  1:1211331/1‑57 (MQ=255)
                                                                                            aGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGa                                                                                                        >  1:1536115/1‑74 (MQ=255)
                                                                                            aGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGa                                                                                                        <  2:1536115/74‑1 (MQ=255)
                                                                                            aGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAAt                                       >  1:1062932/1‑139 (MQ=255)
                                                                                             gcgcAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAAtt                                      <  1:1268228/139‑1 (MQ=255)
                                                                                              cgcAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCt                                                                                                                         <  2:302084/55‑1 (MQ=255)
                                                                                              cgcAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCt                                                                                                                         >  1:302084/1‑55 (MQ=255)
                                                                                                  aGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCg                                 >  1:495230/1‑139 (MQ=255)
                                                                                                             aagaaAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCtt                      >  2:1296328/1‑139 (MQ=255)
                                                                                                               gaaAGGACTAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTtct                    <  2:1062932/139‑1 (MQ=255)
                                                                                                                       tAAAGCGCGTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATcg            <  2:495230/139‑1 (MQ=255)
                                                                                                                              cgTTGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGgatga     >  1:209374/1‑139 (MQ=255)
                                                                                                                                 tGCGCTAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATaa  <  2:209374/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
ATAAATATACATTGTCCTTCTCCTGCAAGAGAATTATTTTAATTTTCGCTTAATTCAGCGAAACCAAAGTAAAGTGGTCAAGCCCCGCGCATAGCGCAAGCTTTGGCAGAAGAAAGGACTAAAGCGCGTTGCGCCAGTGCTGCTCACCTACGAGCAAGATAGTGGATGAGGTACGAAGATGGGTAAATGTAGAAAATAACGTCCTGAACAAATTGTCCATAATATTACAATTATCCGCAGTGTTGCTTCTCGTCATCGCGGATGATAA  >  NC_000913/1673490‑1673757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: