Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,192	G→A	Q447Q (CAG→CAA)	ydgA →	DUF945 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,192	0	G	A	98.0%	149.3 / NA	49	Q447Q (CAG→CAA)	ydgA	DUF945 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/22);  minor base T (0/1);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTT  >  NC_000913/1691070‑1691324                                                                                                                           |                                                                                                                                     aCCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      >  1:456977/1‑139 (MQ=255)      tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGac                                                                                                                        <  2:1403073/132‑1 (MQ=255)      tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGac                                                                                                                        >  1:1403073/1‑132 (MQ=255)             gATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACa                                                                                                          <  1:704767/139‑1 (MQ=255)                atGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATa                                                                                                       >  1:1120656/1‑139 (MQ=255)                       cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTg                                                                                                                 >  2:967574/1‑122 (MQ=255)                       cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTg                                                                                                                 <  1:967574/122‑1 (MQ=255)                          aGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                             >  2:671395/1‑139 (MQ=255)                          aGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                             >  1:132724/1‑139 (MQ=255)                              tATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACGTTGCAGGACAATACCATCACCACAAGc                                                                                         >  2:1357736/1‑139 (MQ=255)                              tATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                         <  2:1220341/139‑1 (MQ=255)                              tATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                         <  1:1231122/139‑1 (MQ=255)                                 gACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                         >  2:1337452/1‑136 (MQ=255)                                 gACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                         <  1:1337452/136‑1 (MQ=255)                                    tCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                   <  2:39347/139‑1 (MQ=255)                                    tCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                   >  1:39347/1‑139 (MQ=255)                                              aaGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTc                                                                                                                                <  2:1446662/84‑1 (MQ=255)                                              aaGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTc                                                                                                                                >  1:1446662/1‑84 (MQ=255)                                                gCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGt                                                                       <  2:1120656/139‑1 (MQ=255)                                                 cTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                                                                   <  2:480700/110‑1 (MQ=255)                                                 cTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                                                                   >  1:480700/1‑110 (MQ=255)                                                 cTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTc                                                                      >  2:481271/1‑139 (MQ=255)                                                     aaGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGAt                                                                  <  2:456977/139‑1 (MQ=255)                                                           aTCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATa                                                                                                       <  1:1337719/96‑1 (MQ=255)                                                           aTCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATa                                                                                                       >  2:1337719/1‑96 (MQ=255)                                                              aGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTaaa                                                         >  2:857865/1‑139 (MQ=255)                                                                  aGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCCTCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTg                                                                                      >  1:154415/1‑106 (MQ=255)                                                                  aGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTg                                                                                      <  2:154415/106‑1 (MQ=255)                                                                   gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      <  1:243736/73‑1 (MQ=255)                                                                   gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGacca                                                                                                                      >  2:243736/1‑73 (MQ=255)                                                                   gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGc                                                    >  1:1459723/1‑139 (MQ=255)                                                                   gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGc                                                    <  2:1459723/139‑1 (MQ=255)                                                                        aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTg                                                                                                                 <  2:422999/73‑1 (MQ=255)                                                                        aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTg                                                                                                                 >  1:422999/1‑73 (MQ=255)                                                                          cGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAAt                                             <  1:857865/139‑1 (MQ=255)                                                                                 aCTGGCGAGACAGCAAGTTGAAGTTGCATCAGCAATGGGGCAAATGTACCGTCTGACCACCTTGCAGGACAAGACCATCACCACCAGCGTGAAATATGCTAACTGTCAGATACCGTTAAATGGGCAGAAAATGTCGTTa                                      <  2:287232/139‑1 (MQ=255)                                                                                    ggCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                         >  1:427152/1‑85 (MQ=255)                                                                                    ggCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                         <  2:427152/85‑1 (MQ=255)                                                                                     gCGAAACAGCAAGTTGAAGTTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACACTACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTagaag                                  >  2:1532955/1‑139 (MQ=255)                                                                                                aGTTGAAGGTGCATCAGCAATGGGGCATATGTTCCGTCTGACCACCATGCAGGACAATTCCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGc                       <  2:132724/139‑1 (MQ=255)                                                                                                   tGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                   >  2:1205511/1‑76 (MQ=255)                                                                                                   tGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                   <  1:1205511/76‑1 (MQ=255)                                                                                                           cATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGc                       >  2:256892/1‑128 (MQ=255)                                                                                                           cATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGc                       <  1:256892/128‑1 (MQ=255)                                                                                                           cATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGAt            >  1:849201/1‑139 (MQ=255)                                                                                                            aTCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATg           >  2:771186/1‑139 (MQ=255)                                                                                                              cAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGcc         <  1:671395/139‑1 (MQ=255)                                                                                                                   aTGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGctc    >  2:752148/1‑139 (MQ=255)                                                                                                                     ggggCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCtt  >  1:1358968/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                     ACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTT  >  NC_000913/1691070‑1691324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑