Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 14,301 C→T A45V (GCG→GTG)  dnaJ → chaperone Hsp40, DnaK co‑chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091314,3010CT98.2% 173.7 / NA 56A45V (GCG→GTG) dnaJchaperone Hsp40, DnaK co‑chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/28);  minor base A (1/0);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTT  >  NC_000913/14175‑14436
                                                                                                                              |                                                                                                                                       
aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAaga                                                                                                                             <  2:125894/139‑1 (MQ=255)
         attaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGa                                                                                                                    <  1:403680/139‑1 (MQ=255)
          ttaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGaa                                                                                                                   >  1:1217314/1‑139 (MQ=255)
               agaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTa                                                                                                              >  2:1137673/1‑139 (MQ=255)
                gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                                             <  1:1137673/139‑1 (MQ=255)
                     tAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGt                                                                                                        <  2:569918/139‑1 (MQ=255)
                     tAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGt                                                                                                        <  2:255271/139‑1 (MQ=255)
                          gTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGa                                                                                                   >  2:7243/1‑139 (MQ=255)
                                       cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                                                      >  1:228976/1‑139 (MQ=255)
                                            gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                 <  2:1217314/139‑1 (MQ=255)
                                                  gAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                           <  1:1424111/139‑1 (MQ=255)
                                                       cAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                      <  2:228976/139‑1 (MQ=255)
                                                       cAGAAAGGCCTACAAACGCCTGACCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                      <  1:1003068/139‑1 (MQ=255)
                                                         gAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAgaga                                                                                                                           <  2:659723/84‑1 (MQ=255)
                                                         gAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAgaga                                                                                                                           >  1:659723/1‑84 (MQ=255)
                                                            aGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAGAAACGTGCGGCATACGATCAGTa                                                                 <  1:1283708/139‑1 (MQ=255)
                                                              gCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATg                                                               >  2:46875/1‑139 (MQ=255)
                                                               ccTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                 <  1:1484406/136‑1 (MQ=255)
                                                               ccTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                 >  2:1484406/1‑136 (MQ=255)
                                                               ccTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATgg                                                              >  2:855445/1‑139 (MQ=255)
                                                                  aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCa                                                                                         >  2:869729/1‑109 (MQ=255)
                                                                  aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCa                                                                                         <  1:869729/109‑1 (MQ=255)
                                                                    aaaCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAagag                                                                                                                            >  1:1078240/1‑72 (MQ=255)
                                                                    aaaCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAagag                                                                                                                            <  2:1078240/72‑1 (MQ=255)
                                                                        gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgctgc                                                     >  1:203897/1‑139 (MQ=255)
                                                                                 tGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCa                                            <  1:894552/139‑1 (MQ=255)
                                                                                 tGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCa                                            <  1:7243/139‑1 (MQ=255)
                                                                                  gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAg                                                                                                                      >  1:513935/1‑64 (MQ=255)
                                                                                  gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAg                                                                                                                      <  2:513935/64‑1 (MQ=255)
                                                                                  gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                                             <  2:5333/73‑1 (MQ=255)
                                                                                  gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                                             >  1:5333/1‑73 (MQ=255)
                                                                                  gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCa                                                                                         >  1:200763/1‑93 (MQ=255)
                                                                                  gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCa                                                                                         <  2:200763/93‑1 (MQ=255)
                                                                                  gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCaa                                           >  2:649626/1‑139 (MQ=255)
                                                                                  gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCaa                                           >  1:1400304/1‑139 (MQ=255)
                                                                                   aaaTACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                          <  1:855445/139‑1 (MQ=255)
                                                                                       accaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                            <  1:1103907/69‑1 (MQ=255)
                                                                                       accaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                            >  2:1103907/1‑69 (MQ=255)
                                                                                       accaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCa                                            >  1:1362877/1‑133 (MQ=255)
                                                                                       accaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCa                                            <  2:1362877/133‑1 (MQ=255)
                                                                                           cccTGACCGTAACCAGGGTGACAAAGAAGACGTGGAGAAATTAAAAGAGATCAATGTAGCTTATGAAGTTCTGACCGACTCGCAAAAACATTCGGCATACGATCACTATGTTCAACCTGCGTTGGAGCAAGGTggagtt                                  >  2:821871/1‑135 (MQ=255)
                                                                                           cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        >  2:1482848/1‑101 (MQ=255)
                                                                                           cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  1:1482848/101‑1 (MQ=255)
                                                                                           cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                      >  1:1377822/1‑103 (MQ=255)
                                                                                           cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                      <  2:1377822/103‑1 (MQ=255)
                                                                                              ggACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggc                               >  2:754395/1‑139 (MQ=255)
                                                                                                          gggTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGa                                                                                                                    <  2:1305312/42‑1 (MQ=255)
                                                                                                          gggTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGa                                                                                                                    >  1:1305312/1‑42 (MQ=255)
                                                                                                               aCAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                <  1:743151/73‑1 (MQ=255)
                                                                                                               aCAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                >  2:743151/1‑73 (MQ=255)
                                                                                                               aCAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        >  1:866693/1‑81 (MQ=255)
                                                                                                               aCAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  2:866693/81‑1 (MQ=255)
                                                                                                               aCAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                                               <  1:15385/106‑1 (MQ=255)
                                                                                                               aCAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                                               >  2:15385/1‑106 (MQ=255)
                                                                                                               aCAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg              >  2:1396978/1‑139 (MQ=255)
                                                                                                                           aGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACtt  >  2:1315330/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                       
AGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTT  >  NC_000913/14175‑14436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑