Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	162,927	G→A	A275T (GCT→ACT)	hrpB →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	162,927	0	G	A	100.0%	191.1 / NA	60	A275T (GCT→ACT)	hrpB	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/29);  total (31/29)

AACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCC  >  NC_000913/162793‑163044                                                                                                                                       |                                                                                                                      aacaacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGa                                                                                                                   >  1:1209738/1‑139 (MQ=255) aacaacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGa                                                                                                                   >  1:232289/1‑139 (MQ=255)   caacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaaa                                                                                                                 <  1:642966/139‑1 (MQ=255)    aacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAc                                                                                                                <  2:1209738/139‑1 (MQ=255)    aacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAc                                                                                                                <  2:232289/139‑1 (MQ=255)    aacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAc                                                                                                                <  1:733324/139‑1 (MQ=255)     acTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAcc                                                                                                               >  1:1233231/1‑139 (MQ=255)          cTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGttt                                                                                                          >  2:803367/1‑139 (MQ=255)           ttCGCGCATCGGTAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTa                                                                                                         <  1:989091/139‑1 (MQ=255)              gcgcATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGATGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAAcc                                                                                                      >  1:1507537/1‑139 (MQ=255)                         gTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTAt                                                                                           <  1:748320/139‑1 (MQ=255)                              gTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                      <  2:1233231/139‑1 (MQ=255)                              gTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                      <  1:803367/139‑1 (MQ=255)                                    ctctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtgg                                                                                >  2:4834/1‑139 (MQ=255)                                      ctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggtg                                                                              >  2:1146658/1‑139 (MQ=255)                                      ctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggt                                                                               <  1:20839/138‑1 (MQ=255)                                      ctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggt                                                                               >  2:20839/1‑138 (MQ=255)                                       tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggtgg                                                                             >  2:160191/1‑139 (MQ=255)                                         cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaaa                                                                                                                 <  2:872233/101‑1 (MQ=255)                                         cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaaa                                                                                                                 >  1:872233/1‑101 (MQ=255)                                         cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAt                                                                           >  2:109212/1‑139 (MQ=255)                                         cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAt                                                                           >  2:1377436/1‑139 (MQ=255)                                          ccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTATTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                          <  2:1087876/139‑1 (MQ=255)                                          ccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                          <  1:109212/139‑1 (MQ=255)                                          ccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                          <  1:1377436/139‑1 (MQ=255)                                            gCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGtt                                                                                                           >  2:488291/1‑104 (MQ=255)                                            gCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGtt                                                                                                           <  1:488291/104‑1 (MQ=255)                                              tGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaaa                                                                                                                 >  2:581219/1‑96 (MQ=255)                                              tGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaaa                                                                                                                 <  1:581219/96‑1 (MQ=255)                                              tGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGa                                                                                                 <  2:566340/112‑1 (MQ=255)                                              tGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGa                                                                                                 >  1:566340/1‑112 (MQ=255)                                                   ggCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAgg                                                                 >  1:505454/1‑139 (MQ=255)                                                       cgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTa                                                                                                         <  1:651659/95‑1 (MQ=255)                                                       cgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTa                                                                                                         >  2:651659/1‑95 (MQ=255)                                                       cgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCg                                                             >  2:964093/1‑139 (MQ=255)                                                          tGTCGCTGAACGATCATCGAAAAGCGATCCTCCCGGCACCGCAAGGGATACGCAAAGGGGTGCTGGCGACCTATATTACTCAAATCAGTTTAACCGTTGAAGGTGTTCGTCTGGTGGTGGATTGTGCCCAGGGGCgtgt                                                          >  2:1198044/1‑139 (MQ=255)                                                          tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAATCCAGTTTa                                                                                                         >  2:133521/1‑92 (MQ=255)                                                          tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAATCCAGTTTa                                                                                                         <  1:133521/92‑1 (MQ=255)                                                          tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGa                                                                                                 >  2:539092/1‑100 (MQ=255)                                                          tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGa                                                                                                 <  1:539092/100‑1 (MQ=255)                                                             cGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgc                                                       <  1:160191/139‑1 (MQ=255)                                                                      aTCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTAt                                                                                           <  1:43163/94‑1 (MQ=255)                                                                      aTCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTAt                                                                                           >  2:43163/1‑94 (MQ=255)                                                                            gAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                        >  2:858378/1‑139 (MQ=255)                                                                                 gCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                      <  2:1044770/88‑1 (MQ=255)                                                                                 gCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                      >  1:1044770/1‑88 (MQ=255)                                                                                                ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                    >  1:104884/1‑139 (MQ=255)                                                                                                 cGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                   <  1:1146658/139‑1 (MQ=255)                                                                                                        gATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCaa            <  2:505454/139‑1 (MQ=255)                                                                                                              cAAAGTGGTGCTGGCGACCAATATTACTGAAACCAg                                                                                                             >  2:317408/1‑36 (MQ=37)                                                                                                              cAAAGTGGTGCTGGCGACCAATATTACTGAAACCAg                                                                                                             <  1:317408/36‑1 (MQ=255)                                                                                                                  gtggtgCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGcc  >  2:697550/1‑139 (MQ=255)                                                                                                                   tggtgCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgc                                                       <  2:1418716/85‑1 (MQ=255)                                                                                                                   tggtgCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgc                                                       >  1:1418716/1‑85 (MQ=255)                                                                                                                           gCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                      <  1:725265/94‑1 (MQ=255)                                                                                                                           gCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                      >  2:725265/1‑94 (MQ=255)                                                                                                                             gACCAATATTACTGAAACCATTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                    <  2:390278/110‑1 (MQ=255)                                                                                                                             gACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCgt                                                            <  1:87951/70‑1 (MQ=255)                                                                                                                             gACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCgt                                                            >  2:87951/1‑70 (MQ=255)                                                                                                                             gACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                    >  1:390278/1‑110 (MQ=255)                                                                                                                                       |                                                                                                                      AACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCC  >  NC_000913/162793‑163044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑