Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,809,787 T→C D136D (GAT→GAC)  yniC → 2‑deoxyglucose‑6‑P phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,809,7870TC100.0% 160.3 / NA 45D136D (GAT→GAC) yniC2‑deoxyglucose‑6‑P phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/21);  total (24/21)

ACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGATGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATCGCCTC  >  NC_000913/1809661‑1809921
                                                                                                                              |                                                                                                                                      
aCCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGAGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcctcg                                                                                                                            >  2:887315/1‑139 (MQ=255)
        tGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaa                                                                                                                    >  2:661203/1‑139 (MQ=255)
         gcgcGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaaa                                                                                                                   >  1:252681/1‑139 (MQ=255)
                             gCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCa                                                                                               <  1:1459958/139‑1 (MQ=255)
                                 aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATc                                                                                             <  2:1380354/137‑1 (MQ=255)
                                 aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATc                                                                                             >  1:1380354/1‑137 (MQ=255)
                                 aGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg                                                                                           <  1:887315/139‑1 (MQ=255)
                                             aTTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATAtctc                                                                               >  2:1155433/1‑139 (MQ=255)
                                                    ggACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTatat                                                                                  <  1:1454995/129‑1 (MQ=255)
                                                    ggACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTatat                                                                                  >  2:1454995/1‑129 (MQ=255)
                                                               cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaaa                                                                                                                   <  2:104168/85‑1 (MQ=255)
                                                               cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGaaaaa                                                                                                                   >  1:104168/1‑85 (MQ=255)
                                                               cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCa                                                                                                      >  2:611518/1‑98 (MQ=255)
                                                               cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCa                                                                                                      <  1:611518/98‑1 (MQ=255)
                                                               cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACt                                                              >  2:279944/1‑138 (MQ=255)
                                                               cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACt                                                              <  1:279944/138‑1 (MQ=255)
                                                               cgcgTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTg                                                             >  1:54619/1‑139 (MQ=255)
                                                                                tGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGAc                                            <  2:252681/139‑1 (MQ=255)
                                                                                    ggAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGc                                        >  1:1206369/1‑139 (MQ=255)
                                                                                     gAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCg                                       >  1:194485/1‑139 (MQ=255)
                                                                                      aaaaaGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGc                                                                                          <  1:1338330/87‑1 (MQ=255)
                                                                                      aaaaaGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGc                                                                                          >  2:1338330/1‑87 (MQ=255)
                                                                                      aaaaaGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGt                                      >  2:1011625/1‑139 (MQ=255)
                                                                                          aGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTgcg                                                                        <  2:353533/101‑1 (MQ=255)
                                                                                          aGTGTTGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTgcg                                                                        >  1:353533/1‑101 (MQ=255)
                                                                                               tGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcct                                                                                                                              >  1:523778/1‑42 (MQ=255)
                                                                                               tGACCATGTTTGACTTACGCGACAGTTTCGACGCcctcgcct                                                                                                                              <  2:523778/42‑1 (MQ=255)
                                                                                               tGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGa                             <  2:194485/139‑1 (MQ=255)
                                                                                               tGACCATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGa                             <  1:1155433/139‑1 (MQ=255)
                                                                                                  ccATGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGa                          <  2:54619/139‑1 (MQ=255)
                                                                                                    aTGTTTGACTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGAtt                        >  1:297401/1‑139 (MQ=255)
                                                                                                           aCTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCg                                       >  1:388225/1‑117 (MQ=255)
                                                                                                           aCTTACGCGACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCg                                       <  2:388225/117‑1 (MQ=255)
                                                                                                                cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATcc                                                                                            >  2:444492/1‑59 (MQ=255)
                                                                                                                cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATcc                                                                                            <  1:444492/59‑1 (MQ=255)
                                                                                                                cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg                                                                                           <  1:383232/60‑1 (MQ=255)
                                                                                                                cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCg                                                                                           >  2:383232/1‑60 (MQ=255)
                                                                                                                cgcgACAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCa            <  1:400148/139‑1 (MQ=255)
                                                                                                                cgcgACAGTTTCGACGACCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCa            >  2:745723/1‑139 (MQ=255)
                                                                                                                     cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTggg                                                           <  1:1053091/87‑1 (MQ=255)
                                                                                                                     cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTggg                                                           >  2:1053091/1‑87 (MQ=255)
                                                                                                                     cAGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATc       <  1:661203/139‑1 (MQ=255)
                                                                                                                      aGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTaa                  >  1:220899/1‑127 (MQ=255)
                                                                                                                      aGTTTCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTaa                  <  2:220899/127‑1 (MQ=255)
                                                                                                                          tCGACGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATCGCctc  >  2:529590/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                      
ACCAGGCGTGCGCGAAGCCGTTGCGTTATGCAAAGAACAAGGTTTATTGGTGGGACTGGCCTCCGCGTCACCACTACATATGCTGGAAAAAGTGTTGACCATGTTTGACTTACGCGACAGTTTCGATGCCCTCGCCTCGGCCGAAAAACTGCCTTACAGCAAGCCGCATCCGCAAGTATATCTCGACTGCGCAGCAAAACTGGGCGTTGACCCTCTGACCTGCGTAGCGCTGGAAGATTCGGTAAATGGCATGATCGCCTC  >  NC_000913/1809661‑1809921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑