Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,826,546	T→C	E126E (GAA→GAG)	astE ←	succinylglutamate desuccinylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,826,546	0	T	C	100.0%	133.4 / NA	38	E126E (GAA→GAG)	astE	succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/18);  total (20/18)

TGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGA  >  NC_000913/1826428‑1826663                                                                                                                       |                                                                                                                      tGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACa                                                                                                   >  2:1075031/1‑139 (MQ=255)                      agAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCaaa                                                                              >  2:1247631/2‑139 (MQ=255)                        aaCTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATa                                                                            >  1:992477/1‑139 (MQ=255)                              ggATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGcc                                                                      >  1:1267251/1‑139 (MQ=255)                                     aGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcgcg                                                                                                          <  1:6701/96‑1 (MQ=255)                                     aGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcgcg                                                                                                          >  2:6701/1‑96 (MQ=255)                                          ccACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCaa                                                          >  1:249069/1‑139 (MQ=255)                                          ccACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCaa                                                          <  2:1267251/139‑1 (MQ=255)                                                            aGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGc                                        <  2:992477/139‑1 (MQ=255)                                                             gATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCCACGACCGCCAAACATTCGATTCATATCGCt                                       <  1:1075031/139‑1 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcgcg                                                                                                          <  2:1350429/66‑1 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTcgcgcg                                                                                                          >  1:1350429/1‑66 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAAc                                                                   >  1:1482381/1‑105 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCg                                                   >  1:745112/1‑121 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCg                                                   <  2:745112/121‑1 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                 >  2:1054783/1‑139 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                 <  2:662905/139‑1 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                 >  2:727186/1‑139 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCa                                 >  1:662905/1‑139 (MQ=255)                                                                   ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCACCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAAc                                                                   <  2:1482381/105‑1 (MQ=255)                                                                     tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAAt                               >  2:355993/1‑139 (MQ=255)                                                                         aGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACg                           <  1:1054783/139‑1 (MQ=255)                                                                         aGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACg                           <  2:249069/139‑1 (MQ=255)                                                                             cacaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGcc                                                                                                        <  2:931887/58‑1 (MQ=255)                                                                             cacaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGcc                                                                                                        >  1:931887/1‑58 (MQ=255)                                                                             cacaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTc                       >  1:1080469/1‑139 (MQ=255)                                                                                        tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGc                                                                                        <  2:149287/63‑1 (MQ=255)                                                                                        tGCCCTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGc                                                                                        >  1:149287/1‑63 (MQ=255)                                                                                           ccTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAgcgc         <  2:534884/139‑1 (MQ=255)                                                                                            cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCttt                                                                                     <  1:944730/62‑1 (MQ=255)                                                                                            cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCttt                                                                                     >  2:944730/1‑62 (MQ=255)                                                                                            cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAAt                                                                             >  2:1045593/1‑70 (MQ=255)                                                                                            cTGGTCATAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAAt                                                                             <  1:1045593/70‑1 (MQ=255)                                                                                                  aTAAAAATCTTCCAGGCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAggagga  <  2:1080469/139‑1 (MQ=255)                                                                                                         tCTTCCAGGCACTGCTCCAGTTCGTGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGc                                                                       <  2:282154/63‑1 (MQ=255)                                                                                                         tCTTCCAGGCACTGCTCCAGTTCGTGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGc                                                                       >  1:282154/1‑63 (MQ=255)                                                                                                                ggCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGAt                                                 >  2:1010990/1‑78 (MQ=255)                                                                                                                ggCACTGCTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGAt                                                 <  1:1010990/78‑1 (MQ=255)                                                                                                                       |                                                                                                                      TGTCGCGTTGCGGTAATACACCGAACTGCGGATGCAAGGAGCCACGAATTGCGGTATGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGA  >  NC_000913/1826428‑1826663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑