Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,874,798 G→A *149* (TAG→TAA
T260I (ACT→ATT) 
yeaL →
yeaM ←
UPF0756 family putative inner membrane protein
putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,874,7980GA100.0% 182.9 / NA 57*149* (TAG→TAA
T260I (ACT→ATT) 
yeaL
yeaM
UPF0756 family putative inner membrane protein
putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/32);  total (25/32)

ATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACT  >  NC_000913/1874667‑1874896
                                                                                                                                   |                                                                                                  
aTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                             <  2:1105055/139‑1 (MQ=255)
    gCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                         <  1:707172/139‑1 (MQ=255)
         cagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAatata                                                                                    <  2:328351/139‑1 (MQ=255)
                      tCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                       >  2:441228/1‑139 (MQ=255)
                        gCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTg                                                                     >  1:135817/1‑139 (MQ=255)
                           gggCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGccc                                                                  <  2:1110598/139‑1 (MQ=255)
                                gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             <  1:441228/139‑1 (MQ=255)
                                         cACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaa                                                    <  1:165819/139‑1 (MQ=255)
                                           cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAAc                                                  >  1:281936/1‑139 (MQ=255)
                                              ttttAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATa                                               <  2:429929/139‑1 (MQ=255)
                                                 tAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGta                                            <  2:135817/139‑1 (MQ=255)
                                                        aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                     <  2:458806/123‑1 (MQ=255)
                                                        aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                     >  1:458806/1‑123 (MQ=255)
                                                           gcTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCg                                  <  2:281936/139‑1 (MQ=255)
                                                           gcTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCg                                  <  1:275102/139‑1 (MQ=255)
                                                                 tCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                             <  1:985281/74‑1 (MQ=255)
                                                                 tCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                             >  2:985281/1‑74 (MQ=255)
                                                                    cggcgTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             >  2:1054559/1‑103 (MQ=255)
                                                                    cggcgTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             <  1:1054559/103‑1 (MQ=255)
                                                                                 gggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                        >  1:1432271/1‑79 (MQ=255)
                                                                                 gggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                        <  2:1432271/79‑1 (MQ=255)
                                                                                 gggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             <  1:166173/90‑1 (MQ=255)
                                                                                 gggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             >  2:166173/1‑90 (MQ=255)
                                                                                 gggCCACTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             <  1:608988/90‑1 (MQ=255)
                                                                                 gggCCACTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             >  2:608988/1‑90 (MQ=255)
                                                                                   gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTa                                                                                                 <  2:1445735/52‑1 (MQ=255)
                                                                                   gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTa                                                                                                 >  1:1445735/1‑52 (MQ=255)
                                                                                   gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCt                                                                             >  2:1316439/1‑72 (MQ=255)
                                                                                   gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCt                                                                             <  1:1316439/72‑1 (MQ=255)
                                                                                   gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                   <  1:177653/98‑1 (MQ=255)
                                                                                   gCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                   >  2:177653/1‑98 (MQ=255)
                                                                                      gCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                         >  2:283056/1‑57 (MQ=255)
                                                                                      gCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                         <  1:283056/57‑1 (MQ=255)
                                                                                      gCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                        <  1:734202/74‑1 (MQ=255)
                                                                                      gCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                        >  2:734202/1‑74 (MQ=255)
                                                                                       cTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAatat                                                                                     <  1:301979/60‑1 (MQ=255)
                                                                                       cTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAatat                                                                                     >  2:301979/1‑60 (MQ=255)
                                                                                        ttATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                     >  2:1159383/1‑91 (MQ=255)
                                                                                        ttATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTa                                                     <  1:1159383/91‑1 (MQ=255)
                                                                                             gCCGCTGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa     <  1:868421/134‑1 (MQ=255)
                                                                                             gCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCa     >  2:868421/1‑134 (MQ=255)
                                                                                                 cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACt  >  2:1300562/1‑133 (MQ=255)
                                                                                                 cgGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACt  <  1:1300562/133‑1 (MQ=255)
                                                                                                  gggTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             <  1:1374380/73‑1 (MQ=255)
                                                                                                  gggTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             >  2:1374380/1‑73 (MQ=255)
                                                                                                   ggTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCg                                                                                           <  1:89601/42‑1 (MQ=255)
                                                                                                   ggTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCg                                                                                           >  2:89601/1‑42 (MQ=255)
                                                                                                   ggTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                         >  2:274415/1‑44 (MQ=255)
                                                                                                   ggTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                         <  1:274415/44‑1 (MQ=255)
                                                                                                   ggTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                   <  2:1002789/82‑1 (MQ=255)
                                                                                                   ggTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                   >  1:1002789/1‑82 (MQ=255)
                                                                                                                  aTTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCt                                                           <  2:411887/59‑1 (MQ=255)
                                                                                                                  aTTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCt                                                           >  1:411887/1‑59 (MQ=255)
                                                                                                                   ttGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                        >  1:1528058/1‑45 (MQ=255)
                                                                                                                   ttGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                        <  2:1528058/45‑1 (MQ=255)
                                                                                                                        gggAAACAGTAATTAATCTCGCAATATATCGGCCTg                                                                            >  2:1102159/1‑36 (MQ=255)
                                                                                                                        gggAAACAGTAATTAATCTCGCAATATATCGGCCTg                                                                            <  1:1102159/36‑1 (MQ=255)
                                                                                                                                   |                                                                                                  
ATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACT  >  NC_000913/1874667‑1874896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: