Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,998,494	C→T	*329* (TAG→TAA)	dcyD ←	D‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,998,494	0	C	T	100.0%	221.4 / NA	70	*329* (TAG→TAA)	dcyD	D‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/36);  total (34/36)

GCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACA  >  NC_000913/1998358‑1998626                                                                                                                                         |                                                                                                                                     gcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTGTACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTa                                                                                                                                    <  2:1115577/139‑1 (MQ=255)            cccGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGgat                                                                                                                         <  2:804212/139‑1 (MQ=255)                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATa                                                                                                                     >  2:172158/1‑139 (MQ=255)                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATa                                                                                                                     >  2:795555/1‑139 (MQ=255)                             aaaaCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCa                                                                                                        >  1:704508/1‑139 (MQ=255)                                      cgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                               <  1:795555/139‑1 (MQ=255)                                               tGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGa                                                                                      <  1:801399/139‑1 (MQ=255)                                                    tGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATaaa                                                                                 <  2:704508/139‑1 (MQ=255)                                                      cAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACa                                                                               >  1:1261623/1‑139 (MQ=255)                                                           tataCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATc                                                                          <  2:1261623/139‑1 (MQ=255)                                                             taCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTaa                                                                                                                                   >  1:672768/1‑80 (MQ=255)                                                             taCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTaa                                                                                                                                   <  2:672768/80‑1 (MQ=255)                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGgat                                                                                                                         >  2:777876/1‑84 (MQ=255)                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGgat                                                                                                                         <  1:777876/84‑1 (MQ=255)                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTc                                                                  >  2:417966/1‑139 (MQ=255)                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTc                                                                  >  1:1253691/1‑139 (MQ=255)                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGa                                                                                      <  2:234631/118‑1 (MQ=255)                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGa                                                                                      >  1:234631/1‑118 (MQ=255)                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                >  1:980095/1‑139 (MQ=255)                                                                        tttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATCAACAGAATCgg                                                                        >  1:1132927/1‑128 (MQ=255)                                                                        tttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCtt                                                             <  1:1245735/139‑1 (MQ=255)                                                                        tttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGACGGTATGAATAAACAGAATCgg                                                                        <  2:1132927/128‑1 (MQ=255)                                                                         ttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAg                                                                                                            <  1:290977/91‑1 (MQ=255)                                                                         ttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAg                                                                                                            >  2:290977/1‑91 (MQ=255)                                                                                   aCGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                   >  2:847867/1‑138 (MQ=255)                                                                                   aCGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                   <  1:847867/138‑1 (MQ=255)                                                                                    cGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTct                                                 <  1:1274290/139‑1 (MQ=255)                                                                                          ggAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTctgactga                                           <  2:442429/139‑1 (MQ=255)                                                                                            aaTCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                           <  2:179462/89‑1 (MQ=255)                                                                                            aaTCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                           >  1:179462/1‑89 (MQ=255)                                                                                              tCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAAc                                                                                                                                 >  2:736786/1‑49 (MQ=255)                                                                                              tCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAAc                                                                                                                                 <  1:736786/49‑1 (MQ=255)                                                                                              tCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                               >  2:621057/1‑83 (MQ=255)                                                                                              tCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                               <  1:621057/83‑1 (MQ=255)                                                                                                 aTAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAg                                                                                                            <  2:432659/67‑1 (MQ=255)                                                                                                 aTAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAg                                                                                                            >  1:432659/1‑67 (MQ=255)                                                                                                      cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                               <  2:1134773/75‑1 (MQ=255)                                                                                                      cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                               >  1:1134773/1‑75 (MQ=255)                                                                                                      cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCa                               >  2:740716/1‑139 (MQ=255)                                                                                                        gTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGc                             >  1:1214715/1‑139 (MQ=255)                                                                                                          tGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGccc                           >  1:206965/1‑139 (MQ=255)                                                                                                            tataCTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTg                                                                                                                              <  2:989362/38‑1 (MQ=255)                                                                                                            tataCTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTg                                                                                                                              >  1:989362/1‑38 (MQ=255)                                                                                                                cTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCAt                     <  2:1214715/139‑1 (MQ=255)                                                                                                                  ttCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCg                   <  2:206965/139‑1 (MQ=255)                                                                                                                   tCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGgcg                                                                                                    >  2:1453562/1‑57 (MQ=255)                                                                                                                   tCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGgcg                                                                                                    <  1:1453562/57‑1 (MQ=255)                                                                                                                   tCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                            >  2:866193/1‑129 (MQ=255)                                                                                                                   tCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                            <  1:866193/129‑1 (MQ=255)                                                                                                                   tCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGc                  >  1:147245/1‑139 (MQ=255)                                                                                                                     ttGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtt                <  1:436404/139‑1 (MQ=255)                                                                                                                      tGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCa                                                                                                  <  2:1246518/56‑1 (MQ=255)                                                                                                                      tGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCa                                                                                                  >  1:1246518/1‑56 (MQ=255)                                                                                                                      tGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCg                                      <  2:601138/116‑1 (MQ=255)                                                                                                                      tGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCg                                      >  1:601138/1‑116 (MQ=255)                                                                                                                           gAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAgcg                                                                                                          >  1:845031/1‑43 (MQ=255)                                                                                                                           gAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAgcg                                                                                                          <  2:845031/43‑1 (MQ=255)                                                                                                                           gAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTct                                                 >  2:338130/1‑100 (MQ=255)                                                                                                                           gAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTct                                                 <  1:338130/100‑1 (MQ=255)                                                                                                                           gAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCgg          >  1:1126553/1‑139 (MQ=255)                                                                                                                             gCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCAt                     <  1:874782/126‑1 (MQ=255)                                                                                                                             gCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCAt                     >  2:874782/1‑126 (MQ=255)                                                                                                                               gTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGt                                                                                          <  1:1194300/55‑1 (MQ=255)                                                                                                                               gTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGt                                                                                          >  2:1194300/1‑55 (MQ=255)                                                                                                                                 ttCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca    <  2:980095/139‑1 (MQ=255)                                                                                                                                  tCCGGAGTTAAACGTGGGGATGATAGGCGAACAgcgc                                                                                                         <  2:768893/37‑1 (MQ=255)                                                                                                                                  tCCGGAGTTAAACGTGGGGATGATAGGCGAACAgcgc                                                                                                         >  1:768893/1‑37 (MQ=255)                                                                                                                                   ccGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca  <  1:172158/139‑1 (MQ=255)                                                                                                                                     ggAGTTAAACGTGGGGATGATAGGCGAACAGCGCAgg                                                                                                      <  2:210529/37‑1 (MQ=255)                                                                                                                                     ggAGTTAAACGTGGGGATGATAGGCGAACAGCGCAgg                                                                                                      >  1:210529/1‑37 (MQ=255)                                                                                                                                         |                                                                                                                                     GCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACA  >  NC_000913/1998358‑1998626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑