Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,090,046	G→A	*17* (TAG→TAA)	hisL →	his operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,090,046	0	G	A	100.0%	281.5 / NA	87	*17* (TAG→TAA)	hisL	his operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/46);  total (41/46)

TATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGA  >  NC_000913/2089913‑2090180                                                                                                                                      |                                                                                                                                       tatataAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCttt                                                                                                                                   <  2:437904/139‑1 (MQ=255)         aaaGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAt                                                                                                                           <  2:1482846/139‑1 (MQ=255)           aGCCCTTGCTTTCTAACGTGAAAGTGGTATAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACTATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgt                                                                                                                         >  2:136047/1‑139 (MQ=255)              ccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                       >  1:1128334/1‑138 (MQ=255)              ccTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtgtgt                                                                                                                       <  2:1128334/138‑1 (MQ=255)                ttGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCt                                                                                                                    >  1:813711/1‑139 (MQ=255)                     ttCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               <  1:916237/139‑1 (MQ=255)                           cGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTGAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                         <  2:392187/139‑1 (MQ=255)                            gTGACAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCa                                                                                                        <  2:1062682/139‑1 (MQ=255)                            gTGAAAGTTGAGTAGGTTATAAGACATCAATTCAATAAAAATTCACAGAGACTTTTATGACACGCGTTCAAATTAAACTCCACCAGGATCACCACCATCCTGGCGAAACATTCAGGCGATGTGTGCTGGAAGACaacca                                                                                                        <  2:60762/139‑5 (MQ=255)                             tGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               <  1:540097/115‑1 (MQ=255)                             tGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               >  2:540097/1‑115 (MQ=255)                               aaaGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGAt                                                                                                     >  2:343124/1‑139 (MQ=255)                                     gTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCa                                                                                               <  1:136047/139‑1 (MQ=255)                                      tttAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  1:316695/139‑1 (MQ=255)                                        tAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      >  2:1531981/1‑129 (MQ=255)                                        tAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  1:1531981/129‑1 (MQ=255)                                           gTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAg                                                                                                                                >  2:744100/1‑100 (MQ=255)                                           gTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAg                                                                                                                                <  1:744100/100‑1 (MQ=255)                                               aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               >  2:829062/1‑97 (MQ=255)                                               aaaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAgg                                                                                                                               <  1:829062/97‑1 (MQ=255)                                                aaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 <  2:589534/110‑1 (MQ=255)                                                aaGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 >  1:589534/1‑110 (MQ=255)                                                 aGACATCAGTTGAATAAACATTCACAGAGTCTTTTATGACACGCGCTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   <  2:988979/139‑1 (MQ=255)                                                 aGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   <  1:343124/139‑1 (MQ=255)                                                    cATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGc                                                                                >  2:349181/1‑139 (MQ=255)                                                     aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      <  1:438282/116‑1 (MQ=255)                                                     aTCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGa                                                                                                      >  2:438282/1‑116 (MQ=255)                                                         gTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCttt                                                                                                                                   <  2:1485965/83‑1 (MQ=255)                                                         gTTGAACAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCttt                                                                                                                                   >  1:1485965/1‑83 (MQ=255)                                                          ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                         >  2:999056/1‑108 (MQ=255)                                                          ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTc                                                                                                         <  1:999056/108‑1 (MQ=255)                                                          ttGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATgaga                                                                          <  1:349181/139‑1 (MQ=255)                                                               tAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               >  1:1100339/1‑97 (MQ=255)                                                               tAAACATTCACAGAGACTTTCATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               <  2:1100339/97‑1 (MQ=255)                                                                    aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               <  1:1316289/92‑1 (MQ=255)                                                                    aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               >  2:1316289/1‑92 (MQ=255)                                                                     ttCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGa                                                               <  2:238441/139‑1 (MQ=255)                                                                      tCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                >  1:704876/1‑89 (MQ=255)                                                                      tCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGaa                                                                                                                <  2:704876/89‑1 (MQ=255)                                                                            agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCt                                                                                                                    >  1:1230930/1‑79 (MQ=255)                                                                            agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCt                                                                                                                    <  2:1230930/79‑1 (MQ=255)                                                                            agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAc                                                        <  2:813711/139‑1 (MQ=255)                                                                              aCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCGTCATCCTGACTAATCTTTCAGGCGATGTGTGCGGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGaa                                                                         <  2:77848/120‑1 (MQ=255)                                                                              aCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGaa                                                                         >  1:77848/1‑120 (MQ=255)                                                                                ttttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 >  2:961303/1‑78 (MQ=255)                                                                                ttttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGa                                                                                                                 <  1:961303/78‑1 (MQ=255)                                                                                  ttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          >  1:45391/1‑67 (MQ=255)                                                                                  ttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGAtg                                                                                                                          <  2:45391/67‑1 (MQ=255)                                                                                      gACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                              >  2:497381/1‑139 (MQ=255)                                                                                          cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               <  2:1172736/70‑1 (MQ=255)                                                                                          cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAg                                                                                                               >  1:1172736/1‑70 (MQ=255)                                                                                          cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                          >  2:1457882/1‑139 (MQ=255)                                                                                          cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttt                                          >  2:357260/1‑139 (MQ=255)                                                                                            cgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACa                                                                                                            <  1:245477/71‑1 (MQ=255)                                                                                            cgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACa                                                                                                            >  2:245477/1‑71 (MQ=255)                                                                                            cgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                        >  2:594355/1‑139 (MQ=255)                                                                                                   tttAAACACCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  1:510718/139‑1 (MQ=255)                                                                                                        acacCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACAt                                                                                                           >  2:1149096/1‑60 (MQ=255)                                                                                                        acacCACCATCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACAt                                                                                                           <  1:1149096/60‑1 (MQ=255)                                                                                                           ccaccaTCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   <  2:797736/81‑1 (MQ=255)                                                                                                           ccaccaTCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   >  1:797736/1‑81 (MQ=255)                                                                                                           ccaccaTCATCACCATCATCCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGAt                         >  2:1422429/1‑139 (MQ=255)                                                                                                             accatcatcaccatcatcCTGACTAATCTTTCAgg                                                                                                                               >  2:99127/1‑35 (MQ=38)                                                                                                             accatcatcaccatcatcCTGACTAATCTTTCAgg                                                                                                                               <  1:99127/35‑1 (MQ=255)                                                                                                                    tcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   <  1:1489451/72‑1 (MQ=255)                                                                                                                    tcaccatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGaa                                                                                   >  2:1489451/1‑72 (MQ=255)                                                                                                                      accatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGa                                                               <  1:1327987/90‑1 (MQ=255)                                                                                                                      accatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGa                                                               >  2:1327987/1‑90 (MQ=255)                                                                                                                      accatcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa              <  2:1461472/139‑1 (MQ=255)                                                                                                                          tcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 <  1:1009063/116‑1 (MQ=255)                                                                                                                          tcatcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcg                                 >  2:1009063/1‑116 (MQ=255)                                                                                                                           catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa              <  2:1331862/134‑1 (MQ=255)                                                                                                                           catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGa              >  1:1331862/1‑134 (MQ=255)                                                                                                                           catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGt         >  1:86959/1‑139 (MQ=255)                                                                                                                           catcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGt         >  2:332576/1‑139 (MQ=255)                                                                                                                             tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATgaga                                                                          >  1:1090434/1‑72 (MQ=255)                                                                                                                             tcCTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATgaga                                                                          <  2:1090434/72‑1 (MQ=255)                                                                                                                               cTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                        <  1:351754/120‑1 (MQ=255)                                                                                                                               cTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                        >  2:351754/1‑120 (MQ=255)                                                                                                                               cTGACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa     <  1:1457882/139‑1 (MQ=255)                                                                                                                                 gACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag   <  2:283384/139‑1 (MQ=255)                                                                                                                                 gACTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAag   <  1:476615/139‑1 (MQ=255)                                                                                                                                  aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              <  1:364913/47‑1 (MQ=255)                                                                                                                                  aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAg                                                                                              >  2:364913/1‑47 (MQ=255)                                                                                                                                  aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga  >  1:1147335/1‑139 (MQ=255)                                                                                                                                  aCTAATCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga  >  2:1475360/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       TATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTAGGTTAAAAGACATCAGTTGAATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGA  >  NC_000913/2089913‑2090180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑