| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,111,150 | G→A | A276V (GCG→GTG) | rfbD ← | dTDP‑L‑rhamnose synthase, NAD(P)‑dependent dTDP‑4‑dehydrorhamnose reductase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,111,150 | 0 | G | A | 98.2% | 179.3 / ‑3.9 | 56 | A276V (GCG→GTG) | rfbD | dTDP‑L‑rhamnose synthase, NAD(P)‑dependent dTDP‑4‑dehydrorhamnose reductase subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (25/30); minor base T (1/0); total (26/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCC > NC_000913/2111014‑2111278 | tttcatttcaTCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGcaca > 1:528343/1‑139 (MQ=255)tttcatttcaTCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGcaca > 2:170675/1‑139 (MQ=255) tcatTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTctgct > 1:618129/1‑139 (MQ=255) catTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTctgctg < 2:340194/139‑1 (MQ=255) ttAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt < 2:618129/139‑1 (MQ=255) aaTTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCt < 1:880248/139‑1 (MQ=255) cTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTaa > 1:855374/1‑139 (MQ=255) gCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc > 2:1076254/1‑139 (MQ=255) gCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGCCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc < 1:1464115/139‑1 (MQ=255) cTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCg > 2:742628/1‑139 (MQ=255) tCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga < 1:572834/139‑1 (MQ=255) caccaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgaga < 1:170675/139‑1 (MQ=255) accaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGcaca < 2:879537/103‑1 (MQ=255) accaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGcaca > 1:879537/1‑103 (MQ=255) ccaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt > 2:1237060/1‑121 (MQ=255) ccaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt < 1:966590/121‑1 (MQ=255) ccaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt < 1:1237060/121‑1 (MQ=255) ccaTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt > 2:966590/1‑121 (MQ=255) caTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGtt < 1:1124101/139‑1 (MQ=255) cACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAgg < 1:232528/128‑1 (MQ=255) cACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAgg > 2:232528/1‑128 (MQ=255) aaCAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGga < 1:1076254/139‑1 (MQ=255) gATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGacgacg > 2:308384/1‑139 (MQ=255) aTGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGAcgacga > 1:301024/1‑139 (MQ=255) tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg < 1:308384/139‑1 (MQ=255) tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg < 1:188678/139‑1 (MQ=255) aaaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt < 2:830838/103‑1 (MQ=255) aaaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAAttttt > 1:830838/1‑103 (MQ=255) aaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgt < 2:615718/139‑1 (MQ=255) aTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAGAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgt > 1:1335508/1‑134 (MQ=255) aTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAGAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgt < 2:1335508/134‑1 (MQ=255) aTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTAtt > 1:387811/1‑103 (MQ=255) aTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTAtt < 2:387811/103‑1 (MQ=255) aTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAgg > 1:1073132/1‑139 (MQ=255) ttAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa > 1:997328/1‑139 (MQ=255) tGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt < 1:742628/139‑1 (MQ=255) cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg > 1:1521594/1‑120 (MQ=255) cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg < 2:1521594/120‑1 (MQ=255) cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgt > 1:1217473/1‑139 (MQ=255) cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgt > 2:1201980/1‑139 (MQ=255) gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTa < 2:1217473/139‑1 (MQ=255) aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGt < 2:1073132/139‑1 (MQ=255) ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg > 1:483185/1‑104 (MQ=255) ttCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAg < 2:483185/104‑1 (MQ=255) tGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCTCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCtt > 1:1453588/1‑139 (MQ=255) gCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCttgtt < 2:528343/139‑1 (MQ=255) gCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCttgtt < 2:301024/139‑1 (MQ=255) cATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGtt < 1:1509281/48‑1 (MQ=255) cATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGtt > 2:1509281/1‑48 (MQ=255) cGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAg > 2:1497620/1‑42 (MQ=255) cGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAg < 1:1497620/42‑1 (MQ=255) tCACGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGCTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTTGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCCAg > 2:1154167/1‑139 (MQ=255) aCGCCAACCTGCCAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAggg > 1:667837/1‑139 (MQ=255) ccAGTCAGGCAAGACAAGCACCAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCt > 1:675215/1‑139 (MQ=255) ccAGTCAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCt > 1:1118402/1‑139 (MQ=255) cAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTgcg < 2:667837/139‑1 (MQ=255) cAGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTgcg < 1:1201980/139‑1 (MQ=255) aGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt > 1:1406329/1‑85 (MQ=255) aGGCAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt < 2:1406329/85‑1 (MQ=255) cAAGACAAGCACAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGcc < 2:997328/139‑1 (MQ=255) | TTTCATTTCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCC > NC_000913/2111014‑2111278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |