Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,157,110 G→A P616P (CCG→CCA mdtB → multidrug efflux system, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,157,1100GA100.0% 273.8 / NA 84P616P (CCG→CCAmdtBmultidrug efflux system, subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/43);  total (41/43)

AGGGCACTTTGCAGGCACCGCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCGTCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTGCCGGGCGTCGATCTCTTCCTGCAACC  >  NC_000913/2156974‑2157241
                                                                                                                                        |                                                                                                                                    
aGGGCACTTTGCAGGCACCGCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATc                                                                                                                                    >  2:1342788/1‑139 (MQ=255)
      cTTTGCAGGCACCGCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGaa                                                                                                                              <  1:1342788/139‑1 (MQ=255)
       tttGCAGGCACCGCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAAc                                                                                                                             >  1:301417/1‑139 (MQ=255)
       tttGCAGGCACCGCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAAc                                                                                                                             >  1:1362453/1‑139 (MQ=255)
         tGCAGGCACCGCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAg                                                                                                                           <  2:624479/139‑1 (MQ=255)
         tGCAGGCACCGCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAg                                                                                                                           <  2:601895/139‑1 (MQ=255)
              gCACCGCAATCCAGCTCCTTTGCTAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCAc                                                                                                                      >  1:1047707/1‑139 (MQ=255)
                   gCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCAc                                                                                                                      <  1:594809/134‑1 (MQ=255)
                   gCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCAc                                                                                                                      >  2:594809/1‑134 (MQ=255)
                    cAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTAc                                                                                                                <  2:1418182/139‑1 (MQ=255)
                       tCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACaaa                                                                                                             >  1:1518360/1‑139 (MQ=255)
                            cTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAAc                                                                                                        >  1:1491697/1‑139 (MQ=255)
                                 ttGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCaa                                                                                                   <  1:1069105/139‑1 (MQ=255)
                                         atGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTgg                                                                                           <  2:1491697/139‑1 (MQ=255)
                                             cccAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGa                                                                                       >  1:1344395/1‑139 (MQ=255)
                                                aGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCa                                                                                                    <  1:1122404/123‑1 (MQ=255)
                                                aGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCa                                                                                                    >  2:1122404/1‑123 (MQ=255)
                                                 gCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGt                                                                                   >  2:618619/1‑139 (MQ=255)
                                                  cGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCg                                                                                                                                   <  1:69958/90‑1 (MQ=255)
                                                  cGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCg                                                                                                                                   >  2:69958/1‑90 (MQ=255)
                                                  cGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGc                                                                                                                        >  1:1444531/1‑101 (MQ=255)
                                                  cGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGc                                                                                                                        <  2:1444531/101‑1 (MQ=255)
                                                  cGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACg                                                                                    >  1:1312511/1‑137 (MQ=255)
                                                  cGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACg                                                                                    <  2:1312511/137‑1 (MQ=255)
                                                           cAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCggg                                                                         >  2:1460267/1‑139 (MQ=255)
                                                           cAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCggg                                                                         <  1:1131883/139‑1 (MQ=255)
                                                             ggTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCAc                                                                                                                      >  2:1510861/1‑92 (MQ=255)
                                                             ggTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCAc                                                                                                                      <  1:1510861/92‑1 (MQ=255)
                                                                  cgGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAAt                                                                                                            >  1:526816/1‑97 (MQ=255)
                                                                  cgGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAAt                                                                                                            <  2:526816/97‑1 (MQ=255)
                                                                  cgGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCaaaa                                                                  >  2:405018/1‑139 (MQ=255)
                                                                  cgGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCaaaa                                                                  >  1:1056375/1‑139 (MQ=255)
                                                                   ggACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCaaaaa                                                                 >  2:1450432/1‑139 (MQ=255)
                                                                               gCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACTGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCggg                                                                         >  1:955879/1‑119 (MQ=255)
                                                                               gCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCggg                                                                         <  2:955879/119‑1 (MQ=255)
                                                                                     tCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACg                                                                                    <  1:1228883/102‑1 (MQ=255)
                                                                                     tCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACg                                                                                    >  2:1228883/1‑102 (MQ=255)
                                                                                     tCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCaa                                              >  2:663137/1‑139 (MQ=255)
                                                                                      ccGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCaaa                                             <  2:1518360/139‑1 (MQ=255)
                                                                                      ccGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCaaa                                             <  1:618619/139‑1 (MQ=255)
                                                                                      ccGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCaaa                                             <  2:1362453/139‑1 (MQ=255)
                                                                                        ggCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcg                                           <  1:663137/139‑1 (MQ=255)
                                                                                        ggCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcg                                           <  2:803941/139‑1 (MQ=255)
                                                                                         gCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcgg                                          <  2:1047707/139‑1 (MQ=255)
                                                                                          cAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcggc                                         <  1:405018/139‑1 (MQ=255)
                                                                                          cAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcggc                                         <  2:587653/139‑1 (MQ=255)
                                                                                          cAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcggc                                         <  2:1344395/139‑1 (MQ=255)
                                                                                          cAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcggc                                         <  2:1177436/139‑1 (MQ=255)
                                                                                             tGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGt                                      <  1:1116683/139‑1 (MQ=255)
                                                                                               cAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCt                                                                                                                                 >  2:173042/1‑47 (MQ=255)
                                                                                               cAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCt                                                                                                                                 <  1:173042/47‑1 (MQ=255)
                                                                                               cAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAg                                    >  1:1090676/1‑139 (MQ=255)
                                                                                                       gACCTCATTTGTTGGCGTTGATGGCACTAACCCATCg                                                                                                                                   <  2:1092833/37‑1 (MQ=255)
                                                                                                       gACCTCATTTGTTGGCGTTGATGGCACTAACCCATCg                                                                                                                                   >  1:1092833/1‑37 (MQ=255)
                                                                                                       gACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAAt                                                                                                            >  2:1526004/1‑60 (MQ=255)
                                                                                                       gACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAAt                                                                                                            <  1:1526004/60‑1 (MQ=255)
                                                                                                       gACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTg                            >  1:363563/1‑139 (MQ=255)
                                                                                                       gACCTCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTg                            >  1:706914/1‑139 (MQ=255)
                                                                                                           tCATTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTGCCgg                        <  2:363563/139‑1 (MQ=255)
                                                                                                             aTTTGTTGGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAATTAAACCCCAACCCTTTGAATGACGGTGATGATCGGGTGAAAAAAGCCATGCACGTCCTCAAAACGGCTTAAGATAAAGGCCggggc                     >  1:986009/1‑137 (MQ=255)
                                                                                                                  ttgGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACg                                                                                    <  2:368641/73‑1 (MQ=255)
                                                                                                                  ttgGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACg                                                                                    >  1:368641/1‑73 (MQ=255)
                                                                                                                  ttgGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGt                                      >  1:276422/1‑118 (MQ=255)
                                                                                                                  ttgGCGTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGt                                      <  2:276422/118‑1 (MQ=255)
                                                                                                                       gTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcggc                                         <  2:277251/110‑1 (MQ=255)
                                                                                                                       gTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAAcggc                                         >  1:277251/1‑110 (MQ=255)
                                                                                                                       gTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTGCCGGGCGTCGATctct            >  2:1240320/1‑139 (MQ=255)
                                                                                                                       gTTGATGGCACTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTGCCGGGCGTCGATctct            >  1:1069162/1‑139 (MQ=255)
                                                                                                                                aCTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCg                                                                           <  2:1365612/68‑1 (MQ=255)
                                                                                                                                aCTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCg                                                                           >  1:1365612/1‑68 (MQ=255)
                                                                                                                                 cTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCaaa                                             <  1:386391/96‑1 (MQ=255)
                                                                                                                                 cTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCaaa                                             >  2:386391/1‑96 (MQ=255)
                                                                                                                                 cTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTGCCGGGCGTCGATCTCTTCCTGcaacc  <  1:1450432/139‑1 (MQ=255)
                                                                                                                                 cTAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTGCCGGGCGTCGATCTCTTCCTGcaacc  <  1:1460267/139‑1 (MQ=255)
                                                                                                                                  tAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCg                                                                           <  1:1104311/66‑1 (MQ=255)
                                                                                                                                  tAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCg                                                                           >  2:1104311/1‑66 (MQ=255)
                                                                                                                                  tAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCAt                                                            >  1:1157361/1‑81 (MQ=255)
                                                                                                                                  tAACCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCAt                                                            <  2:1157361/81‑1 (MQ=255)
                                                                                                                                    aCCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCaaa                                             >  2:274394/1‑93 (MQ=255)
                                                                                                                                    aCCCATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCaaa                                             <  1:274394/93‑1 (MQ=255)
                                                                                                                                     cccATCGCTGAACAGTGCACGTTTACAAATCAACCTCa                                                                                                    >  2:1484982/1‑38 (MQ=255)
                                                                                                                                     cccATCGCTGAACAGTGCACGTTTACAAATCAACCTCa                                                                                                    <  1:1484982/38‑1 (MQ=255)
                                                                                                                                     cccATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCa                                               <  2:1520085/90‑1 (MQ=255)
                                                                                                                                     cccATCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCa                                               >  1:1520085/1‑90 (MQ=255)
                                                                                                                                        |                                                                                                                                    
AGGGCACTTTGCAGGCACCGCAATCCAGCTCCTTTGCCAATATGGCCCAGCGACAACGCCAGGTCGCGGACGTGATTTTGCAGGATCCGGCAGTGCAAAGCCTGACCTCATTTGTTGGCGTTGATGGCACTAACCCGTCGCTGAACAGTGCACGTTTACAAATCAACCTCAAACCGTTGGATGAACGTGATGATCGGGTGCAAAAAGTCATCGCCCGTCTGCAAACGGCGGTAGATAAAGTGCCGGGCGTCGATCTCTTCCTGCAACC  >  NC_000913/2156974‑2157241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: