Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,182,035 C→T L314L (CTA→TTA) 
*249* (TAG→TAA) 		 yegV →
yegW ←		 putative kinase
putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,182,0350CT100.0% 222.6 / NA 68L314L (CTA→TTA) 
*249* (TAG→TAA) 		yegV
yegW		putative kinase
putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/33);  total (35/33)

CATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTG  >  NC_000913/2181906‑2182160
                                                                                                                                 |                                                                                                                             
cATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCg                                                                                                                      <  2:831076/139‑1 (MQ=255)
        tGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCg                                                                                                                      <  2:415284/131‑1 (MQ=255)
        tGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCg                                                                                                                      >  1:415284/1‑131 (MQ=255)
        tGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACaaa                                                                                                              >  2:95154/1‑139 (MQ=255)
                          ggCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCg                                                                                            >  1:1258199/1‑139 (MQ=255)
                                     tGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCACCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTaa                                                                                 >  2:428176/1‑139 (MQ=255)
                                       ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGAc                                                                                          <  2:221766/128‑1 (MQ=255)
                                       ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGAc                                                                                          >  1:221766/1‑128 (MQ=255)
                                       ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGt                                                                                       <  2:239899/131‑1 (MQ=255)
                                       ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGt                                                                                       >  1:239899/1‑131 (MQ=255)
                                          cTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTg                                                                            >  2:424374/1‑139 (MQ=255)
                                           tGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACAC‑AAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTa                                                                          <  2:301551/139‑1 (MQ=255)
                                            ggCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGa                                                                                           >  1:52824/1‑122 (MQ=255)
                                            ggCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGa                                                                                           <  2:52824/122‑1 (MQ=255)
                                             gCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTAc                                                                         >  1:1183478/1‑139 (MQ=255)
                                             gCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTAc                                                                         >  2:647598/1‑139 (MQ=255)
                                                   gCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATa                                                                   <  1:267225/139‑1 (MQ=255)
                                                         ttACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGt                                                             <  1:647598/139‑1 (MQ=255)
                                                               ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGt                                                       >  1:1398288/1‑139 (MQ=255)
                                                                 cAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTg                                                     >  1:848353/1‑139 (MQ=255)
                                                                    tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                  >  2:239018/1‑139 (MQ=255)
                                                                    tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                  >  2:807621/1‑139 (MQ=255)
                                                                    tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                  >  1:64728/1‑139 (MQ=255)
                                                                    tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                  >  2:376914/1‑139 (MQ=255)
                                                                      cAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                              >  1:826210/1‑125 (MQ=255)
                                                                      cAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                              <  2:826210/125‑1 (MQ=255)
                                                                       aGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCACCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                               <  1:428176/139‑1 (MQ=255)
                                                                          ggCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcgc                                            <  1:424374/139‑1 (MQ=255)
                                                                             gtcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAc                                         <  2:64728/139‑1 (MQ=255)
                                                                              tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAct                                        >  1:1165106/1‑139 (MQ=255)
                                                                              tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAct                                        <  2:1165106/139‑1 (MQ=255)
                                                                               cgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                       <  1:95154/139‑1 (MQ=255)
                                                                                   ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGcacaca                                                                                                                <  2:45749/62‑1 (MQ=255)
                                                                                   ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGcacaca                                                                                                                >  1:45749/1‑62 (MQ=255)
                                                                                   ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGa                                                                                           <  2:970258/83‑1 (MQ=255)
                                                                                   ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGa                                                                                           >  1:970258/1‑83 (MQ=255)
                                                                                   ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgctt                                   <  2:1183478/139‑1 (MQ=255)
                                                                                   ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgctt                                   >  2:85994/1‑139 (MQ=255)
                                                                                    gTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                  <  1:807621/139‑1 (MQ=255)
                                                                                       gTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAAc                                                                                                           >  2:812797/1‑63 (MQ=255)
                                                                                       gTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAAc                                                                                                           <  1:812797/63‑1 (MQ=255)
                                                                                       gTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                               >  2:531289/1‑123 (MQ=255)
                                                                                       gTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                               <  1:531289/123‑1 (MQ=255)
                                                                                       gTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                  >  1:1509608/1‑136 (MQ=255)
                                                                                       gTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                  <  2:1509608/136‑1 (MQ=255)
                                                                                        tCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                    >  2:1294655/1‑85 (MQ=255)
                                                                                        tCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                    <  1:1294655/85‑1 (MQ=255)
                                                                                            gCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAg                          <  2:1258199/139‑1 (MQ=255)
                                                                                            gCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAg                          <  2:848353/139‑1 (MQ=255)
                                                                                                cGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGa                                                                                           >  2:218465/1‑70 (MQ=255)
                                                                                                cGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGa                                                                                           <  1:218465/70‑1 (MQ=255)
                                                                                                 ggggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCg                     <  2:1398288/139‑1 (MQ=255)
                                                                                                  gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAg                                                                                        <  2:317604/71‑1 (MQ=255)
                                                                                                  gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAg                                                                                        >  1:317604/1‑71 (MQ=255)
                                                                                                  gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCt                                                                             <  1:1488390/82‑1 (MQ=255)
                                                                                                  gggCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCt                                                                             >  2:1488390/1‑82 (MQ=255)
                                                                                                        tGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcg                                             >  2:124500/1‑108 (MQ=255)
                                                                                                        tGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcg                                             <  1:124500/108‑1 (MQ=255)
                                                                                                                cgcCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCt                                                                             >  2:625146/1‑68 (MQ=255)
                                                                                                                cgcCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCt                                                                             <  1:625146/68‑1 (MQ=255)
                                                                                                                  ccAACGCGCGAGGAATTACTCCTCGCACACAAAAACGtataa                                                                                                     <  1:1471916/42‑2 (MQ=255)
                                                                                                                  ccAACGCGCGAGGAATTACTCCTCGCACACAAAAACGtataa                                                                                                     >  2:1471916/1‑41 (MQ=255)
                                                                                                                    aaCGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTg  >  1:758823/1‑139 (MQ=255)
                                                                                                                    aaCGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTg  >  1:986040/1‑139 (MQ=255)
                                                                                                                      cgcgcgAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGt                                                                           <  2:618258/64‑1 (MQ=255)
                                                                                                                      cgcgcgAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGt                                                                           >  1:618258/1‑64 (MQ=255)
                                                                                                                        cgcgAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                    <  1:172142/53‑1 (MQ=255)
                                                                                                                        cgcgAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                    >  2:172142/1‑53 (MQ=255)
                                                                                                                                 |                                                                                                                             
CATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTG  >  NC_000913/2181906‑2182160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑