Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	197,574	T→C	S343S (AGT→AGC)	rseP →	inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	197,574	0	T	C	100.0%	220.6 / NA	62	S343S (AGT→AGC)	rseP	inner membrane zinc RIP metalloprotease, RpoE activator, by degrading RseA, cleaved signal peptide endoprotease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/32);  total (30/32)

TGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGT  >  NC_000913/197442‑197708                                                                                                                                     |                                                                                                                                       tGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTg                                                                                                                                  >  1:510293/1‑139 (MQ=255)    ttttGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGa                                                                                                                               <  1:1252016/139‑1 (MQ=255)            gTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGAt                                                                                                                       <  1:1312878/139‑1 (MQ=255)                tGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCAcc                                                                                                                   >  2:1102770/1‑139 (MQ=255)                   gCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCAc                                                                                                                    >  1:230541/1‑135 (MQ=255)                   gCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCAc                                                                                                                    <  2:230541/135‑1 (MQ=255)                    ccGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAATTTGATCACCGGTg                                                                                                               >  1:450351/1‑139 (MQ=255)                       aaaGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                            >  1:1485803/1‑139 (MQ=255)                            cATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGaaa                                                                                                       <  2:510293/139‑1 (MQ=255)                             aTTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAAc                                                                                                      >  1:714553/1‑139 (MQ=255)                              ttCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACt                                                                                                     >  1:1454624/1‑139 (MQ=255)                               tCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTg                                                                                                    >  2:1069990/1‑139 (MQ=255)                                    tGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCAc                                                                                                                    >  2:119174/1‑118 (MQ=255)                                    tGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCAc                                                                                                                    <  1:119174/118‑1 (MQ=255)                                          aTGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAATTTGATCACCGGTGATGTGAAACTGAACAACCTCAg                                                                                         <  2:450351/139‑1 (MQ=255)                                           tGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGt                                                                                        >  1:758493/1‑139 (MQ=255)                                               tataAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGc                                                                                    >  1:1317562/1‑139 (MQ=255)                                               tataAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGc                                                                                    <  1:1069990/139‑1 (MQ=255)                                                 taAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATACTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCg                                                                                  <  1:1442304/139‑1 (MQ=255)                                                   aaGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGAt                                                                                <  1:986074/139‑1 (MQ=255)                                                         tACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTAt                                                                          >  1:1278985/1‑139 (MQ=255)                                                         tACGCCAGTATGGGACGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCCGCTGATGAAGCTGACGGTCAGCCTGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAAACTCAGTGGGCCGATCTCTAt                                                                          >  2:1306610/1‑139 (MQ=255)                                                               aGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCaa                                                                    <  2:1454624/139‑1 (MQ=255)                                                               aGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCaa                                                                    <  2:1125126/139‑1 (MQ=255)                                                               aGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCaa                                                                    >  2:806752/1‑139 (MQ=255)                                                                     ggCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGc                                                              <  1:806752/139‑1 (MQ=255)                                                                        cGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTg                                                                                                                                  >  1:370984/1‑68 (MQ=255)                                                                        cGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTg                                                                                                                                  <  2:370984/68‑1 (MQ=255)                                                                          ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTg                                                                                                    >  1:349540/1‑96 (MQ=255)                                                                          ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTg                                                                                                    <  2:349540/96‑1 (MQ=255)                                                                          ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATctct                                                                            <  1:178837/120‑1 (MQ=255)                                                                          ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATctct                                                                            >  2:178837/1‑120 (MQ=255)                                                                          ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                         >  1:631716/1‑139 (MQ=255)                                                                          ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                         >  1:507691/1‑139 (MQ=255)                                                                          ttCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGa                                                         >  2:719950/1‑139 (MQ=255)                                                                               cGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACa                                                    >  1:977442/1‑139 (MQ=255)                                                                                  cATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGc                                                                                                                                    >  1:1215431/1‑56 (MQ=255)                                                                                  cATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGc                                                                                                                                    <  2:1215431/56‑1 (MQ=255)                                                                                    tcgtcgAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                            <  1:569298/78‑1 (MQ=255)                                                                                    tcgtcgAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGAtg                                                                                                            >  2:569298/1‑78 (MQ=255)                                                                                       tcgAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACt                                            <  1:1102770/139‑1 (MQ=255)                                                                                         gAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGcc                                                                                   <  2:212098/98‑1 (MQ=255)                                                                                         gAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGcc                                                                                   >  1:212098/1‑98 (MQ=255)                                                                                         gAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCg                                          <  2:1485803/139‑1 (MQ=255)                                                                                            gCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       <  2:1317562/139‑1 (MQ=255)                                                                                            gCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       <  1:1172084/139‑1 (MQ=255)                                                                                             ccACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                      <  1:719950/139‑1 (MQ=255)                                                                                             ccACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                      <  2:714553/139‑1 (MQ=255)                                                                                             ccACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                      <  2:507691/139‑1 (MQ=255)                                                                                             ccACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGgt                                      <  2:1363884/139‑1 (MQ=255)                                                                                                            ggCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       <  2:244479/123‑1 (MQ=255)                                                                                                            ggCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCgggg                                       >  1:244479/1‑123 (MQ=255)                                                                                                             gCAGCTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCg                      >  2:690187/1‑139 (MQ=255)                                                                                                                 cTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTg                                                                                                               >  2:445764/1‑46 (MQ=255)                                                                                                                 cTGATGAAGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTg                                                                                                               <  1:445764/46‑1 (MQ=255)                                                                                                                       aaGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGc                                                              <  2:1154549/89‑1 (MQ=255)                                                                                                                       aaGCTGACGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGc                                                              >  1:1154549/1‑89 (MQ=255)                                                                                                                              cGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                              >  2:236492/1‑50 (MQ=255)                                                                                                                              cGGTCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGaacaac                                                                                              <  1:236492/50‑1 (MQ=255)                                                                                                                                 tCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGAtct                                                                              >  2:1527023/1‑63 (MQ=255)                                                                                                                                 tCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGAtct                                                                              <  1:1527023/63‑1 (MQ=255)                                                                                                                                 tCAGCATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGt  <  1:159810/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TGGTTTTGTCGGTATTGAGCCGAAAGTCATTCCTTTGCCAGATGAGTATAAAGTTGTACGCCAGTATGGGCCGTTCAACGCCATCGTCGAAGCCACGGACAAAACGTGGCAGCTGATGAAGCTGACGGTCAGTATGCTGGGAAAATTGATCACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATCTCTATCGCCAAGGGGGCTGGGATGACAGCGGAACTCGGGGTTGTTTATTACCTGCCGTTTCTTGCGCTTATTAGCGT  >  NC_000913/197442‑197708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑