Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,195,459	T→C	V387A (GTT→GCT)	metG →	methionyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,195,459	0	T	C	100.0%	197.5 / NA	56	V387A (GTT→GCT)	metG	methionyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

GCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGA  >  NC_000913/2195329‑2195591                                                                                                                                   |                                                                                                                                     gCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAg                                                                                                                              >  1:750734/1‑139 (MQ=255)  ccAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGt                                                                                                                             >  1:1184425/1‑139 (MQ=255)    aGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           <  2:750734/139‑1 (MQ=255)    aGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           >  1:1086117/1‑139 (MQ=255)                         gACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATg                                                                                                      <  2:1503167/139‑1 (MQ=255)                                    ccTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCg                                                                                                           >  2:77007/1‑123 (MQ=255)                                    ccTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCg                                                                                                           <  1:77007/123‑1 (MQ=255)                                            actactacACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           >  1:138224/1‑99 (MQ=255)                                            actactacACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           <  2:138224/99‑1 (MQ=255)                                                  acacTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGa                                                                             >  2:646662/1‑139 (MQ=255)                                                      tGCGAAACTCTCTTCGCGCATTGATGATGTCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATCCCGATATCGCTATCAAAGTGGTTAACCTGGCCTCCGGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGc                                                                         >  2:377334/1‑139 (MQ=255)                                                      tGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 >  1:1534719/1‑99 (MQ=255)                                                      tGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 <  2:1534719/99‑1 (MQ=255)                                                      tGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGc                                                                         >  1:1247839/1‑139 (MQ=255)                                                       gCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 <  2:1253314/98‑1 (MQ=255)                                                       gCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 >  1:1253314/1‑98 (MQ=255)                                                        cGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 >  1:578726/1‑97 (MQ=255)                                                        cGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGc                                                                                                                 <  2:578726/97‑1 (MQ=255)                                                        cGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGt                                                                       >  2:1484850/1‑139 (MQ=255)                                                          aaaCTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGc                                                                     >  2:446874/1‑139 (MQ=255)                                                                tctTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGAc                                                                            >  1:550311/1‑126 (MQ=255)                                                                tctTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGAc                                                                            <  2:550311/126‑1 (MQ=255)                                                                          ttGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCggg                                                                                                  >  1:447920/1‑94 (MQ=255)                                                                          ttGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCggg                                                                                                  <  2:447920/94‑1 (MQ=255)                                                                                 tatCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCCACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              <  2:1086117/139‑1 (MQ=255)                                                                                 tatCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCg                                              <  2:1184425/139‑1 (MQ=255)                                                                                   tCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCa                                            >  2:1204949/1‑139 (MQ=255)                                                                                       tctcAACCTGGAAGGTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAACGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGACTGACCCGCAGTTg                                        >  1:941517/1‑139 (MQ=255)                                                                                       tctcAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTg                                        >  2:214868/1‑139 (MQ=255)                                                                                           aaCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa                                    >  2:491430/1‑139 (MQ=255)                                                                                            aCCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACaa                                   <  2:1247839/139‑1 (MQ=255)                                                                                                ggAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAAcc                               >  2:589225/1‑139 (MQ=255)                                                                                                 gAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCaa                                                               >  1:261586/1‑106 (MQ=255)                                                                                                 gAAGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCaa                                                               <  2:261586/106‑1 (MQ=255)                                                                                                  aaGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCtt                             >  1:308287/1‑139 (MQ=255)                                                                                                  aaGATTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCtt                             >  1:813879/1‑139 (MQ=255)                                                                                                     aTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACg                                                                           >  1:605300/1‑90 (MQ=255)                                                                                                     aTTTCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACg                                                                           <  2:605300/90‑1 (MQ=255)                                                                                                       ttCGTTCAGCGTGTGAATGCCGATATCGCTAACaa                                                                                                                                <  1:520118/35‑1 (MQ=255)                                                                                                       ttCGTTCAGCGTGTGAATGCCGATATCGCTAACaa                                                                                                                                >  2:520118/1‑35 (MQ=255)                                                                                                        tCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGa                       <  2:308287/139‑1 (MQ=255)                                                                                                        tCGTTCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGa                       <  2:813879/139‑1 (MQ=255)                                                                                                           ttCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           <  1:1431025/36‑1 (MQ=255)                                                                                                           ttCAGCGTGTGAATGCCGATATCGCTAACAAAGTgg                                                                                                                           >  2:1431025/1‑36 (MQ=255)                                                                                                           ttCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGAcc                                                >  2:626422/1‑111 (MQ=255)                                                                                                           ttCAGCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGAcc                                                <  1:626422/111‑1 (MQ=255)                                                                                                               gCGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCt                <  1:214868/139‑1 (MQ=255)                                                                                                                cGTGTGAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAAACTTCACTGATGCCgacg               >  2:386993/1‑136 (MQ=255)                                                                                                                  tgtgAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATg                                                                                                      <  1:1525660/50‑1 (MQ=255)                                                                                                                  tgtgAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATg                                                                                                      >  2:1525660/1‑50 (MQ=255)                                                                                                                  tgtgAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGtt                                         <  2:206216/111‑1 (MQ=255)                                                                                                                  tgtgAATGCCGATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGtt                                         >  1:206216/1‑111 (MQ=255)                                                                                                                            gATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTg   <  1:446874/139‑1 (MQ=255)                                                                                                                            gATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTg   <  2:79601/139‑1 (MQ=255)                                                                                                                            gATATCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTg   <  1:491430/139‑1 (MQ=255)                                                                                                                             atatCGCTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGa  <  2:941517/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     GCCAGCACCTGGCTGAATCATTTTGACGCAGACAGCCTGCGTTACTACTACACTGCGAAACTCTCTTCGCGCATTGATGATATCGATCTCAACCTGGAAGATTTCGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACAAAACCTTCACTGATGCCGCTGAAGTGATTGGTGA  >  NC_000913/2195329‑2195591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑