Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,200,899	A→G	E207E (GAA→GAG)	yehI →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,200,899	0	A	G	100.0%	199.0 / NA	57	E207E (GAA→GAG)	yehI	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)

TTCGATCTCCGACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAAAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGCCAGGA  >  NC_000913/2200765‑2201030                                                                                                                                       |                                                                                                                                    ttCGATCTCCGACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAAc                                                                                                                                 <  1:761074/139‑1 (MQ=255) tccGATCTCCGACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAAc                                                                                                                                 >  1:508235/3‑139 (MQ=255)           gACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGc                                                                                                                       <  1:460291/139‑1 (MQ=255)                cGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATg                                                                                                                  >  2:660981/1‑139 (MQ=255)                cGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATg                                                                                                                  <  2:508235/139‑1 (MQ=255)                                      agGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGccc                                                                                            >  2:328650/1‑139 (MQ=255)                                          aaGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGa                                                                                        <  1:328650/139‑1 (MQ=255)                                            gctgctGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAAc                                                                                      >  1:77831/1‑139 (MQ=255)                                                  ggCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAttt                                                                                <  1:231290/139‑1 (MQ=255)                                                   gCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAtttt                                                                               <  2:77831/139‑1 (MQ=255)                                                   gCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAtttt                                                                               <  1:155253/139‑1 (MQ=255)                                                        gTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTgg                                                                                                            >  2:1231044/1‑105 (MQ=255)                                                        gTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTgg                                                                                                            <  1:1231044/105‑1 (MQ=255)                                                          tGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              <  2:954115/133‑1 (MQ=255)                                                          tGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              >  1:954115/1‑133 (MQ=255)                                                              ccGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGa                                                                    <  1:660981/139‑1 (MQ=255)                                                                                 aCTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCACGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCt                                                 >  2:442677/1‑139 (MQ=255)                                                                                   tACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGCGGTTAAAAGTTGTTGCTaa                                               >  1:1379839/1‑139 (MQ=255)                                                                                     ccAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAttt                                                                                >  1:1325275/1‑104 (MQ=255)                                                                                     ccAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAttt                                                                                <  2:1325275/104‑1 (MQ=255)                                                                                             aaaCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCcaca                                     >  2:550922/1‑139 (MQ=255)                                                                                             aaaCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCcaca                                     <  1:1211295/139‑1 (MQ=255)                                                                                               aCAAAGTTCGTCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGc                                   >  2:1136268/1‑139 (MQ=255)                                                                                               aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATTGCCAATGAGTTGGTAGTCCTTGaa                                                                                                 <  1:938031/77‑1 (MQ=255)                                                                                               aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGaa                                                                                                 >  2:938031/1‑77 (MQ=255)                                                                                               aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCa                                                                             <  1:65552/97‑1 (MQ=255)                                                                                               aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCa                                                                             >  2:65552/1‑97 (MQ=255)                                                                                               aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGc                                   >  2:427157/1‑139 (MQ=255)                                                                                                   aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTgg                                                                                                            >  1:1259824/1‑62 (MQ=255)                                                                                                   aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTgg                                                                                                            <  2:1259824/62‑1 (MQ=255)                                                                                                   aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              >  2:857243/1‑92 (MQ=255)                                                                                                   aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              <  1:857243/92‑1 (MQ=255)                                                                                                   aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              <  2:1103823/92‑1 (MQ=255)                                                                                                   aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              >  1:1103823/1‑92 (MQ=255)                                                                                                    gTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGa                                                                                                                             <  1:336379/44‑1 (MQ=255)                                                                                                    gTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGa                                                                                                                             >  2:336379/1‑44 (MQ=255)                                                                                                        gccgccCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGaaa                          >  1:613378/1‑139 (MQ=255)                                                                                                          cgccCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGTCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAAc                        <  2:782597/139‑1 (MQ=255)                                                                                                             ccTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGcc                                                                                                                      >  1:839787/1‑42 (MQ=255)                                                                                                             ccTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGcc                                                                                                                      <  2:839787/42‑1 (MQ=255)                                                                                                               ttttATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGaa                   <  1:838232/139‑1 (MQ=255)                                                                                                                 ttATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAaca                 <  1:550922/139‑1 (MQ=255)                                                                                                                       cgcTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTg           >  2:342095/1‑139 (MQ=255)                                                                                                                        gcTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTgg          >  1:1411077/1‑139 (MQ=255)                                                                                                                         cTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACt                                                                                     <  2:263464/63‑1 (MQ=255)                                                                                                                         cTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACt                                                                                     >  1:263464/1‑63 (MQ=255)                                                                                                                         cTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGa         >  2:225426/1‑139 (MQ=255)                                                                                                                          tGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTTATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        <  1:427157/139‑1 (MQ=255)                                                                                                                            aTCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGa                                            <  2:255753/101‑1 (MQ=255)                                                                                                                            aTCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGa                                            >  1:255753/1‑101 (MQ=255)                                                                                                                            aTCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGcc      <  1:1246801/139‑1 (MQ=255)                                                                                                                            aTCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGcc      <  1:1136268/139‑1 (MQ=255)                                                                                                                                tccCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGCCAGGa  <  2:1379839/139‑1 (MQ=255)                                                                                                                                 cccGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        <  2:1107942/132‑1 (MQ=255)                                                                                                                                 cccGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        <  1:172741/132‑1 (MQ=255)                                                                                                                                 cccGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        >  2:172741/1‑132 (MQ=255)                                                                                                                                 cccGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        >  1:1107942/1‑132 (MQ=255)                                                                                                                                       |                                                                                                                                    TTCGATCTCCGACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAAAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGCCAGGA  >  NC_000913/2200765‑2201030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑