Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,233,597	G→A	*240* (TAG→TAA)	sanA →	DUF218 superfamily vancomycin high temperature exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,233,597	0	G	A	100.0%	165.6 / NA	52	*240* (TAG→TAA)	sanA	DUF218 superfamily vancomycin high temperature exclusion protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/26);  total (26/26)

TTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAC  >  NC_000913/2233463‑2233723                                                                                                                                       |                                                                                                                               tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTAt                                                                                                                            >  2:136754/1‑139 (MQ=255) tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTAt                                                                                                                            <  2:810359/139‑1 (MQ=255) tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTAt                                                                                                                            <  2:1219531/139‑1 (MQ=255) tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTAt                                                                                                                            <  1:426984/139‑1 (MQ=255)  ttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATg                                                                                                                           >  2:1415401/1‑139 (MQ=255)                               cTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTa                                                                                                                     >  1:1178548/1‑116 (MQ=255)                               cTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTa                                                                                                                     <  2:1178548/116‑1 (MQ=255)                               cTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGt                                                                                              >  1:819437/1‑139 (MQ=255)                                          tCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTa                                                                                   <  1:525694/139‑1 (MQ=255)                                             ggCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATcc                                                                                <  1:136754/139‑1 (MQ=255)                                                  tGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCg                                                                                               >  2:206914/1‑119 (MQ=255)                                                  tGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCg                                                                                               <  1:206914/119‑1 (MQ=255)                                                      ccAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATAtttt                                                                       >  2:1472086/1‑139 (MQ=255)                                                        aGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGt                                                                     >  1:1227727/1‑139 (MQ=255)                                                            aCCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTcc                                                                 >  1:145345/1‑139 (MQ=255)                                                             ccGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcg                                                                <  2:1068438/139‑1 (MQ=255)                                                                 aaGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGaa                                                            >  2:1195390/1‑139 (MQ=255)                                                                          aGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGaaa                                                   <  1:1472086/139‑1 (MQ=255)                                                                           ggggTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcg                                                                <  2:666685/125‑1 (MQ=255)                                                                           ggggTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcg                                                                >  1:666685/1‑125 (MQ=255)                                                                                 tCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGgc                                            >  1:946795/1‑139 (MQ=255)                                                                                    cgccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGgcgcg                                         <  1:1415401/139‑1 (MQ=255)                                                                                      ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt                                                                                           >  2:1112531/1‑87 (MQ=255)                                                                                      ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtttt                                                                                           <  1:1112531/87‑1 (MQ=255)                                                                                      ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCa                                       >  2:94597/1‑139 (MQ=255)                                                                                        gTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc                                                                                 <  1:673454/95‑1 (MQ=255)                                                                                        gTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc                                                                                 >  2:673454/1‑95 (MQ=255)                                                                                          cacaCCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcg                                   <  2:145345/139‑1 (MQ=255)                                                                                           acacCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcgc                                  <  2:1227727/139‑1 (MQ=255)                                                                                                  aaCAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAgg                                     <  1:738579/129‑1 (MQ=255)                                                                                                  aaCAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAgg                                     >  2:738579/1‑129 (MQ=255)                                                                                                       ttACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcg                                   >  2:454474/1‑126 (MQ=255)                                                                                                       ttACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcg                                   <  1:454474/126‑1 (MQ=255)                                                                                                         aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGc                                                                                                 >  1:267476/1‑62 (MQ=255)                                                                                                         aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGc                                                                                                 <  2:267476/62‑1 (MQ=255)                                                                                                         aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACggg                    >  2:119447/1‑139 (MQ=255)                                                                                                         aCTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACggg                    >  1:1355282/1‑139 (MQ=255)                                                                                                            tGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGt                                                                     <  2:583927/87‑1 (MQ=255)                                                                                                            tGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGt                                                                     >  1:583927/1‑87 (MQ=255)                                                                                                                ttACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTaaaaa             <  2:819437/139‑1 (MQ=255)                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAAt            >  2:189349/1‑139 (MQ=255)                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAAt            >  2:399714/1‑139 (MQ=255)                                                                                                                     aaaGAAACAAGGAAAGTAATTATGTATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCAc        >  2:648202/1‑139 (MQ=255)                                                                                                                     aaaGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCAc        >  2:71892/1‑139 (MQ=255)                                                                                                                         aaaCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGa    >  2:366877/1‑139 (MQ=255)                                                                                                                         aaaCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGa    <  1:71892/139‑1 (MQ=255)                                                                                                                          aaCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGaa   <  1:399714/139‑1 (MQ=255)                                                                                                                           aCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGCAAGGCTGGCCCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc  <  2:946795/139‑1 (MQ=255)                                                                                                                           aCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc  <  2:1355282/139‑1 (MQ=255)                                                                                                                           aCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc  <  1:1195390/139‑1 (MQ=255)                                                                                                                                  aaGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgcg                                                              <  1:855608/72‑1 (MQ=255)                                                                                                                                  aaGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgcg                                                              >  2:855608/1‑72 (MQ=255)                                                                                                                                       |                                                                                                                               TTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAC  >  NC_000913/2233463‑2233723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑