Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	219,364	T→C	P77P (CCA→CCG)	tsaA ←	tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	219,364	0	T	C	100.0%	174.6 / NA	50	P77P (CCA→CCG)	tsaA	tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/23);  total (27/23)

AGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAG  >  NC_000913/219233‑219489                                                                                                                                    |                                                                                                                              aGCTTTAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGca                                                                                                                        <  2:751279/139‑1 (MQ=255)  gCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGcac                                                                                                                       <  1:114457/139‑1 (MQ=255)   cTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGcaca                                                                                                                      >  2:1263926/1‑139 (MQ=255)                   tCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgccgc                                                                                                      >  2:848554/1‑139 (MQ=255)                             aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                                        <  1:870469/127‑1 (MQ=255)                             aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                                        >  2:870469/1‑127 (MQ=255)                             aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCAt                                                                                               >  1:1123211/1‑136 (MQ=255)                             aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCAt                                                                                               <  2:1123211/136‑1 (MQ=255)                                                 aCCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGa                                                                        >  2:1306472/1‑139 (MQ=255)                                                  ccAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                       >  1:1070935/1‑139 (MQ=255)                                                       gACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATa                                                                  >  2:787532/1‑139 (MQ=255)                                                       gACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATa                                                                  >  1:749338/1‑139 (MQ=255)                                                          aTGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAAt                                                               >  1:1380323/1‑139 (MQ=255)                                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGa                                                                                                                <  1:531604/88‑1 (MQ=255)                                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGa                                                                                                                >  2:531604/1‑88 (MQ=255)                                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                       <  1:636485/129‑1 (MQ=255)                                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                       >  2:636485/1‑129 (MQ=255)                                                            gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATgg                                                             >  2:1106127/1‑139 (MQ=255)                                                               aaTTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTg                                                          <  1:1263926/139‑1 (MQ=255)                                                                    ggTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTcc                                                                                                 >  1:541699/1‑95 (MQ=255)                                                                    ggTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTcc                                                                                                 <  2:541699/95‑1 (MQ=255)                                                                          ggCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAg                                               >  1:1508523/1‑139 (MQ=255)                                                                                  gTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGaa                                       <  1:1106127/139‑1 (MQ=255)                                                                                    agagCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACg                                     >  1:59641/1‑139 (MQ=255)                                                                                     gagCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACgg                                    <  1:1306472/139‑1 (MQ=255)                                                                                         gtgtTTCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTc                                >  2:1504952/1‑139 (MQ=255)                                                                                           gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCgg                              <  1:787532/139‑1 (MQ=255)                                                                                            ttGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGTACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGc                             >  2:349875/1‑139 (MQ=255)                                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGca                                                                                                                        >  1:234804/1‑47 (MQ=255)                                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGca                                                                                                                        <  2:234804/47‑1 (MQ=255)                                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                       <  2:562070/96‑1 (MQ=255)                                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                       >  1:562070/1‑96 (MQ=255)                                                                                             tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGcc                            <  2:1070935/139‑1 (MQ=255)                                                                                                 aaatCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                            <  1:956731/71‑1 (MQ=255)                                                                                                 aaaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                            >  2:956731/1‑71 (MQ=255)                                                                                                  aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGaa                                       <  1:754461/123‑1 (MQ=255)                                                                                                  aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGaa                                       >  2:754461/1‑123 (MQ=255)                                                                                                     cccccATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGa                                                                                                                >  1:932954/1‑47 (MQ=255)                                                                                                     cccccATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGa                                                                                                                <  2:932954/47‑1 (MQ=255)                                                                                                     cccccATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTa                    <  1:297048/139‑1 (MQ=255)                                                                                                        ccATTCTGGCGTTACCGCCGAGGCGCGGCGTACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAggg                 <  1:349875/139‑1 (MQ=255)                                                                                                         cATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                           <  2:254322/80‑1 (MQ=255)                                                                                                         cATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                           >  1:254322/1‑80 (MQ=255)                                                                                                            tCTGGCGTTACCACCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCa             <  1:747624/139‑1 (MQ=255)                                                                                                                gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATTCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATga         >  2:971370/1‑139 (MQ=255)                                                                                                                   ttACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGc                                                            <  1:884120/85‑1 (MQ=255)                                                                                                                   ttACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGc                                                            >  2:884120/1‑85 (MQ=255)                                                                                                                       cgccgAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAg  >  2:1475004/1‑139 (MQ=255)                                                                                                                          cgAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATg          <  2:526806/128‑1 (MQ=255)                                                                                                                          cgAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATg          >  1:526806/1‑128 (MQ=255)                                                                                                                                    |                                                                                                                              AGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAG  >  NC_000913/219233‑219489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑