Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,358,163 G→A A228V (GCG→GTG)  rhmA ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,358,1630GA100.0% 224.8 / NA 70A228V (GCG→GTG) rhmA2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/31);  total (39/31)

TGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGT  >  NC_000913/2358029‑2358289
                                                                                                                                      |                                                                                                                               
tGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAg                                                                                                                             >  1:923583/1‑139 (MQ=255)
      tttGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtg                                                                                                                       >  1:1343857/1‑139 (MQ=255)
       ttGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgc                                                                                                                      <  2:1008205/139‑1 (MQ=255)
        tGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgct                                                                                                                     >  1:618342/1‑139 (MQ=255)
          atatCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                   <  1:66603/139‑1 (MQ=255)
            atCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtg                                                                                                                       >  1:1071216/1‑133 (MQ=255)
            atCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtg                                                                                                                       <  2:1071216/133‑1 (MQ=255)
               aaTAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCat                                                                                                              >  2:602449/1‑139 (MQ=255)
                aTAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCata                                                                                                             <  1:1043700/139‑1 (MQ=255)
                 tAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCatat                                                                                                            >  2:1508393/1‑139 (MQ=255)
                    cTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCACTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAg                                                                                                         <  2:618342/139‑1 (MQ=255)
                            tATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑Ccaca                                                                                                <  2:1343857/139‑1 (MQ=255)
                                 gTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCCACAGCCa                                                                                            >  1:1415486/1‑139 (MQ=255)
                                  tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtg                                                                                                                       <  1:1327097/111‑1 (MQ=255)
                                  tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtg                                                                                                                       >  2:1327097/1‑111 (MQ=255)
                                    gCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGTATTGCTGCTCCATAACAGGAG‑CCACAGCCAGaaa                                                                                        >  1:682763/1‑139 (MQ=255)
                                     cccATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCACGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGaaaa                                                                                       >  1:343584/1‑139 (MQ=255)
                                                   aTTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCTTTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTAcc                                                                         <  2:923583/139‑1 (MQ=255)
                                                   aTTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCata                                                                                                             <  1:1476915/104‑1 (MQ=255)
                                                   aTTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCata                                                                                                             >  2:1476915/1‑104 (MQ=255)
                                                    tttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTAccc                                                                        >  1:244971/1‑139 (MQ=255)
                                                             cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                   >  2:1073582/1‑88 (MQ=255)
                                                             cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                   <  1:1073582/88‑1 (MQ=255)
                                                             cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgct                                                                                                                     <  2:1001442/86‑1 (MQ=255)
                                                             cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgct                                                                                                                     >  1:1001442/1‑86 (MQ=255)
                                                             cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAg                                                                                                         >  2:1383461/1‑98 (MQ=255)
                                                             cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAg                                                                                                         <  1:1383461/98‑1 (MQ=255)
                                                             cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACgg                                                               >  2:591221/1‑139 (MQ=255)
                                                                              gggCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGt                                              <  1:591221/139‑1 (MQ=255)
                                                                                gCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGttt                                            <  1:602449/139‑1 (MQ=255)
                                                                                gCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGttt                                            <  2:244971/139‑1 (MQ=255)
                                                                                    cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaat                                        >  2:1344051/1‑139 (MQ=255)
                                                                                    cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaat                                        >  2:287222/1‑139 (MQ=255)
                                                                                    cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaat                                        >  2:1070639/1‑139 (MQ=255)
                                                                                              cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑cc                                                                                                   >  2:1359775/1‑70 (MQ=255)
                                                                                              cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑cc                                                                                                   <  1:1359775/70‑1 (MQ=255)
                                                                                              cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGc                              >  2:946546/1‑139 (MQ=255)
                                                                                              cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGc                              >  1:771228/1‑139 (MQ=255)
                                                                                                tCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCata                                                                                                             <  2:305826/59‑1 (MQ=255)
                                                                                                tCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCata                                                                                                             >  1:305826/1‑59 (MQ=255)
                                                                                                 cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAgg                                                                                                                            <  2:295995/43‑1 (MQ=255)
                                                                                                 cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAgg                                                                                                                            >  1:295995/1‑43 (MQ=255)
                                                                                                 cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGcc                                                                                                                >  1:694805/1‑55 (MQ=255)
                                                                                                 cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGcc                                                                                                                <  2:694805/55‑1 (MQ=255)
                                                                                                 cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                       <  2:103753/95‑1 (MQ=255)
                                                                                                 cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                       >  1:103753/1‑95 (MQ=255)
                                                                                                 cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACt                           >  1:360310/1‑139 (MQ=255)
                                                                                                 cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACt                           >  2:396597/1‑139 (MQ=255)
                                                                                                   tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGAt                                                             <  1:265730/103‑1 (MQ=255)
                                                                                                   tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGAt                                                             >  2:265730/1‑103 (MQ=255)
                                                                                                   tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg                                                          >  2:639000/1‑106 (MQ=255)
                                                                                                   tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg                                                          <  1:639000/106‑1 (MQ=255)
                                                                                                   tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc                         >  1:573560/1‑139 (MQ=255)
                                                                                                   tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc                         >  1:573512/1‑139 (MQ=255)
                                                                                                     tCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑Ccac                                                                                                 >  2:1476361/1‑65 (MQ=255)
                                                                                                     tCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑Ccac                                                                                                 <  1:1476361/65‑1 (MQ=255)
                                                                                                        aCGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑c                                                                                                    >  2:124876/1‑59 (MQ=255)
                                                                                                        aCGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑c                                                                                                    <  1:124876/59‑1 (MQ=255)
                                                                                                           ccAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑Ccac                                                                                                 >  2:1240747/1‑59 (MQ=255)
                                                                                                           ccAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑Ccac                                                                                                 <  1:1240747/59‑1 (MQ=255)
                                                                                                           ccAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGcc                 <  1:1070639/139‑1 (MQ=255)
                                                                                                                    aCAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACt                           >  1:1073527/1‑120 (MQ=255)
                                                                                                                    aCAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACt                           <  2:1073527/120‑1 (MQ=255)
                                                                                                                        aGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCgg    >  1:614653/1‑139 (MQ=255)
                                                                                                                         gTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAACCCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCggg   <  2:682763/139‑1 (MQ=255)
                                                                                                                         gTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCggg   <  2:771228/139‑1 (MQ=255)
                                                                                                                          ttCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCggg   <  2:1415486/139‑1 (MQ=255)
                                                                                                                          ttCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGt  >  1:648501/1‑139 (MQ=255)
                                                                                                                          ttCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGt  <  2:360310/139‑1 (MQ=255)
                                                                                                                                  ccACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCgcag                                                                    >  2:711465/1‑65 (MQ=255)
                                                                                                                                  ccACACCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCgcag                                                                    <  1:711465/65‑1 (MQ=255)
                                                                                                                                      |                                                                                                                               
TGGGCCTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAG‑CCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGT  >  NC_000913/2358029‑2358289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑