Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,019	T→C	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA → arnD →	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,019	0	T	C	100.0%	156.0 / NA	44	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA arnD	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/21);  total (23/21)

CGTAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAAT  >  NC_000913/2369883‑2370149                                                                                                                                         |                                                                                                                                   cGTAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGa                                                                                                                                  >  2:975410/1‑139 (MQ=255) cGTAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGa                                                                                                                                  >  2:1071420/1‑139 (MQ=255) cGTAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGa                                                                                                                                  >  1:288178/1‑139 (MQ=255)  gTAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAACCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATCTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                 >  1:1526983/1‑139 (MQ=255)  gTAGAGCATCGGAAACCGAGCATCCGCAATGCGCACGCCTGCCTAGACTGGTAGACGAAAATTGATATGCAGGAATCCATCGACGCAACGCTGGGTTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                 <  2:1526983/139‑1 (MQ=255)                aCCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTAc                                                                                                                   >  1:1369262/1‑139 (MQ=255)                 ccGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACg                                                                                                                  <  2:552890/139‑1 (MQ=255)                    aGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAgg                                                                                                                        >  2:958425/1‑130 (MQ=255)                    aGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAgg                                                                                                                        <  1:958425/130‑1 (MQ=255)                     gCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                              <  1:1071420/139‑1 (MQ=255)                              aTGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGAt                                                                                                     <  1:1370131/139‑1 (MQ=255)                                      cGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCg                                                                                             >  2:409750/1‑139 (MQ=255)                                      cGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCg                                                                                             >  2:40021/1‑139 (MQ=255)                                          gCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                         <  1:1043330/139‑1 (MQ=255)                                           ccTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAGACGCTGGATTTCTTCCTGCGCGCCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                       >  2:1360231/1‑108 (MQ=255)                                           ccTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAGACGCTGGATTTCTTCCTGCGCGCCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                       <  1:1360231/108‑1 (MQ=255)                                                     gAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                              >  2:1215181/1‑107 (MQ=255)                                                     gAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                              <  1:1215181/107‑1 (MQ=255)                                                          gAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGc                                                                         >  2:582478/1‑139 (MQ=255)                                                                 gATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTct                                                                  <  1:409750/139‑1 (MQ=255)                                                                             aCCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                              >  1:1320451/1‑83 (MQ=255)                                                                             aCCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                              <  2:1320451/83‑1 (MQ=255)                                                                               cATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGa                                                    >  2:1031466/1‑139 (MQ=255)                                                                                 tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                  >  2:1458508/1‑139 (MQ=255)                                                                                   gacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                <  1:975410/139‑1 (MQ=255)                                                                                    acgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                 >  1:1204705/1‑57 (MQ=255)                                                                                    acgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                 <  2:1204705/57‑1 (MQ=255)                                                                                    acgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAg                                               <  1:40021/139‑1 (MQ=255)                                                                                        aaCGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGa                                                                                                                                  <  1:834861/52‑1 (MQ=255)                                                                                         aCGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCcgc                                                                      >  1:1469542/1‑111 (MQ=255)                                                                                         aCGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCcgc                                                                      <  2:1469542/111‑1 (MQ=255)                                                                                                    cttcCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCg                                                                                    <  1:1441087/86‑1 (MQ=255)                                                                                                    cttcCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCg                                                                                    >  2:1441087/1‑86 (MQ=255)                                                                                                     ttcCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                              <  1:174540/59‑1 (MQ=255)                                                                                                     ttcCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                              >  2:174540/1‑59 (MQ=255)                                                                                                     ttcCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAg                              >  1:335293/1‑139 (MQ=255)                                                                                                         tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                  >  1:1399380/1‑115 (MQ=255)                                                                                                         tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                  <  2:1399380/115‑1 (MQ=255)                                                                                                                  ttGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                <  2:706513/108‑1 (MQ=255)                                                                                                                  ttGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                >  1:706513/1‑108 (MQ=255)                                                                                                                        ttACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGc           <  1:451668/139‑1 (MQ=255)                                                                                                                                 aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGt                                                                    <  1:1310046/73‑1 (MQ=255)                                                                                                                                 aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGt                                                                    >  2:1310046/1‑73 (MQ=255)                                                                                                                                 aaCCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAat  >  2:1056891/1‑139 (MQ=255)                                                                                                                                      tcacGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                <  2:851776/88‑1 (MQ=255)                                                                                                                                      tcacGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                >  1:851776/1‑88 (MQ=255)                                                                                                                                         |                                                                                                                                   CGTAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAAT  >  NC_000913/2369883‑2370149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑