Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,379,348	C→T	*432* (TAG→TAA)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,348	0	C	T	100.0%	141.5 / NA	44	*432* (TAG→TAA)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/22);  total (22/22)

AATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCT  >  NC_000913/2379218‑2379480                                                                                                                                   |                                                                                                                                     aaTGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                              <  2:1131576/139‑1 (MQ=255) aaTGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                              <  1:1481610/139‑1 (MQ=255)  aTGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAtt                                                                                                                             <  2:951830/139‑1 (MQ=255)           gccCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATaaa                                                                                                                    <  1:1359409/139‑1 (MQ=255)                           aTGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCa                                                                                                             >  2:226245/1‑130 (MQ=255)                           aTGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCa                                                                                                             <  1:226245/130‑1 (MQ=255)                                cTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTttg                                                                                               >  2:1524793/1‑139 (MQ=255)                                             gTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCt                                                                                  >  2:486013/1‑139 (MQ=255)                                              tttGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTa                                                                                                                 <  2:516143/107‑1 (MQ=255)                                              tttGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTa                                                                                                                 >  1:516143/1‑107 (MQ=255)                                               ttGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGc                                                                                <  1:486013/139‑1 (MQ=255)                                                tGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                              <  1:1030974/92‑1 (MQ=255)                                                tGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                              >  2:1030974/1‑92 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATaaa                                                                                                                    >  1:1426975/1‑85 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATaaa                                                                                                                    <  2:1426975/85‑1 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                     <  2:190988/132‑1 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                     >  1:190988/1‑132 (MQ=255)                                                                 gCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                              >  1:777275/1‑139 (MQ=255)                                                                  cGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                              <  2:589442/74‑1 (MQ=255)                                                                  cGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                              >  1:589442/1‑74 (MQ=255)                                                                  cGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGcc                                                                                                          <  2:295521/94‑1 (MQ=255)                                                                  cGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGcc                                                                                                          >  1:295521/1‑94 (MQ=255)                                                                     cTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTttgt                                                                                              >  2:559793/1‑103 (MQ=255)                                                                     cTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTttgt                                                                                              <  1:559793/103‑1 (MQ=255)                                                                                    tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                           >  2:1061516/1‑139 (MQ=255)                                                                                       aaaaaTAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                        <  1:259044/139‑1 (MQ=255)                                                                                       aaaaaTAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                        <  1:1061516/139‑1 (MQ=255)                                                                                                    tGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCg                           >  2:1449531/1‑139 (MQ=255)                                                                                                             tCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACAtt                  >  2:259875/1‑139 (MQ=255)                                                                                                             tCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACAtt                  >  2:1001802/1‑139 (MQ=255)                                                                                                                gTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGcc                                                                                                          <  2:334484/48‑1 (MQ=255)                                                                                                                gTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGcc                                                                                                          >  1:334484/1‑48 (MQ=255)                                                                                                                gTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGcc               >  2:1197313/1‑139 (MQ=255)                                                                                                                   atgatgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCt            <  2:1259507/139‑1 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                          >  1:1327607/1‑101 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcgc                                          <  2:1327607/101‑1 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                        >  1:1398978/1‑103 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                        <  2:1398978/103‑1 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                        >  2:1008924/1‑103 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                        <  1:1008924/103‑1 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg    >  2:148535/1‑139 (MQ=255)                                                                                                                           aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg    >  1:361840/1‑139 (MQ=255)                                                                                                                             tCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCt  <  1:1449531/139‑1 (MQ=255)                                                                                                                             tCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCt  <  1:259875/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     AATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCT  >  NC_000913/2379218‑2379480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑