Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,105	G→A	pseudogene (291/291 nt) P138L (CCT→CTT)	tfaS → yfdK ←	pseudogene, CPS‑53 (KpLE1) prophage, tail fiber assembly protein fragment,Phage or Prophage Related CPS‑53 (KpLE1) prophage, conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,105	0	G	A	100.0%	198.5 / NA	62	pseudogene (291/291 nt) P138L (CCT→CTT)	tfaS yfdK	pseudogene, CPS‑53 (KpLE1) prophage, tail fiber assembly protein fragment,Phage or Prophage Related CPS‑53 (KpLE1) prophage, conserved protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

ATTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAGGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTG  >  NC_000913/2470970‑2471232                                                                                                                                        |                                                                                                                                aTTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGcc                                                                                                                              <  1:728774/139‑1 (MQ=255)            ggCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGc                                                                                                                               >  2:1460707/1‑127 (MQ=255)            ggCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCt                                                                                                                   >  2:1195424/1‑139 (MQ=255)             gCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTg                                                                                                                  <  1:544344/139‑1 (MQ=255)                  aGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCa                                                                                                             <  2:1207034/139‑1 (MQ=255)                      gATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTgg                                                                                                         >  2:996236/1‑139 (MQ=255)                            ggcagAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                   <  1:301329/139‑1 (MQ=255)                             gcagAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATc                                                                                                  >  2:756198/1‑139 (MQ=255)                              cagAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCg                                                                                                 <  1:972477/139‑1 (MQ=255)                                 agaCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                               <  2:1426317/138‑1 (MQ=255)                                 agaCCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                               >  1:1426317/1‑138 (MQ=255)                                       cccGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                   <  2:1042002/128‑1 (MQ=255)                                       cccGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTcc                                                                                        >  1:1083399/1‑139 (MQ=255)                                       cccGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTcc                                                                                        >  1:135166/1‑139 (MQ=255)                                       cccGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTcc                                                                                        >  1:346025/1‑139 (MQ=255)                                       cccGACTTAACACTGTGCTGCATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTAt                                                                                                   >  1:1042002/1‑128 (MQ=255)                                          gACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGt                                                                                     <  2:346025/139‑1 (MQ=255)                                          gACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGt                                                                                     <  2:258382/139‑1 (MQ=255)                                                acacTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTcc                                                                               >  1:1213661/1‑139 (MQ=255)                                                      tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCAt                                                                                                                            <  2:1353162/88‑1 (MQ=25)                                                      tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCAt                                                                                                                            >  1:1353162/1‑88 (MQ=18)                                                      tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                               >  1:166324/1‑117 (MQ=255)                                                      tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGAc                                                                                               <  2:166324/117‑1 (MQ=255)                                                      tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCg                                                                                  >  2:1052008/1‑130 (MQ=255)                                                      tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCg                                                                                  <  1:1052008/130‑1 (MQ=255)                                                      tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATaa                                                                         >  1:1193391/1‑139 (MQ=255)                                                      tgCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATaa                                                                         >  1:452885/1‑139 (MQ=255)                                                       gCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTTCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAAt                                                                        >  1:337273/1‑139 (MQ=255)                                                          ggATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCa                                                                     <  1:1195424/139‑1 (MQ=255)                                                           gATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAg                                                                    <  1:996236/139‑1 (MQ=255)                                                             ttACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                  <  2:1213661/139‑1 (MQ=255)                                                               aCATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCcac                                                                <  1:756198/139‑1 (MQ=255)                                                                  ttGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACaaa                                                             >  1:206077/1‑139 (MQ=255)                                                                     aCGCGGTAACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATta                                                          >  1:467444/1‑139 (MQ=255)                                                                             aCGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTgcgc                                                  <  2:467444/139‑1 (MQ=255)                                                                                          ccAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCt                                     <  2:452885/139‑1 (MQ=255)                                                                                                 cgcgCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                  <  2:222260/103‑1 (MQ=255)                                                                                                 cgcgCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                  >  1:222260/1‑103 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGTAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAgac                              >  2:267440/1‑137 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGTAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAgac                              <  1:267440/137‑1 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGgcgc                                                                                                              >  2:1319819/1‑57 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGgcgc                                                                                                              <  1:1319819/57‑1 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                      <  1:192051/81‑1 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAg                                                                                      >  2:192051/1‑81 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCg                                                                                  <  2:597430/85‑1 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCg                                                                                  >  1:597430/1‑85 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCt                                          <  1:993342/125‑1 (MQ=255)                                                                                                   cgcCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCt                                          >  2:993342/1‑125 (MQ=255)                                                                                                           gTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGc                                                                   >  1:1032320/1‑92 (MQ=255)                                                                                                           gTCATCTGGCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGc                                                                   <  2:1032320/92‑1 (MQ=255)                                                                                                                  ggCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCt                                          >  2:1496088/1‑110 (MQ=255)                                                                                                                  ggCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCt                                          <  1:1496088/110‑1 (MQ=255)                                                                                                                  ggCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTa             >  2:865987/1‑139 (MQ=255)                                                                                                                   gCCTGAACTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTAc            <  1:865987/139‑1 (MQ=255)                                                                                                                        aaCTGCCGGAGGAGTAAGCCATTCAATATCTGgcgc                                                                                                              <  1:1171873/36‑1 (MQ=255)                                                                                                                        aaCTGCCGGAGGAGTAAGCCATTCAATATCTGgcgc                                                                                                              >  2:1171873/1‑36 (MQ=14)                                                                                                                         aCTGCCGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGccc      <  2:206077/139‑1 (MQ=255)                                                                                                                             ccGGAGGAGTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTg  >  1:1186778/1‑139 (MQ=255)                                                                                                                                  ggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                  <  2:816816/70‑1 (MQ=255)                                                                                                                                  ggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGcc                                                                  >  1:816816/1‑70 (MQ=255)                                                                                                                                  ggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACaaa                                                             >  2:1219909/1‑75 (MQ=255)                                                                                                                                  ggagTAAGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACaaa                                                             <  1:1219909/75‑1 (MQ=255)                                                                                                                                        |                                                                                                                                ATTACAGGCTGGGCGGAAGCTGATGCAGGCAGAGACCTCCCGACTTAACACTGTGCTGGATTACATTGACGCGGTGACGGCAACAGATACCAGCACCGCGCCGGATGTCATCTGGCCTGAACTGCCGGAGGAGTAGGCCATTCAATATCTGGCGCACTGGAAGTATCGACCAGCTCCAGTGCGTCCAGATAATCCAGCCACAAATTATATTGCGCCAGTTCCTCACCTTTCAGACGACCAATAGCCGCTTTACCAGCCCATTG  >  NC_000913/2470970‑2471232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑