Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,511,468	C→T	*413* (TAG→TAA)	mntH ←	manganese/divalent cation transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,511,468	0	C	T	100.0%	120.3 / NA	38	*413* (TAG→TAA)	mntH	manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/18);  total (20/18)

TCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAA  >  NC_000913/2511333‑2511600                                                                                                                                        |                                                                                                                                     tCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                                   <  2:32354/139‑1 (MQ=255)     ccAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATc                                                                                                                               >  2:12318/1‑139 (MQ=255)         tGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAg                                                                                                                           >  1:58587/1‑139 (MQ=255)            aGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAgcgc                                                                                                                        >  1:650788/1‑139 (MQ=255)                caATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTc                                                                                                                    >  1:94189/1‑139 (MQ=255)                   tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTcccc                                                                                                                 >  1:1065510/1‑139 (MQ=255)                        gATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaa                                                                                                            <  2:650788/139‑1 (MQ=255)                        gATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaa                                                                                                            <  2:1065510/139‑1 (MQ=255)                          tGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                          <  2:94189/139‑1 (MQ=255)                              aCGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGTTCATTCAATTACAATCCCAGCGCCGTccc                                                                                                                  <  2:886845/127‑1 (MQ=255)                              aCGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTccc                                                                                                                  >  1:886845/1‑127 (MQ=255)                                 cTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaac                                                                                                           <  1:1446145/131‑1 (MQ=255)                                 cTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaac                                                                                                           >  2:1446145/1‑131 (MQ=255)                                   aGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGatat                                                                                                 >  1:1311554/1‑139 (MQ=255)                                     tGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTc                                                                                               >  2:336691/1‑139 (MQ=255)                                           ttATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGcc                                                                                         <  2:1311554/139‑1 (MQ=255)                                                              gCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACATTCCCGGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCa                                                                      >  1:1068359/1‑139 (MQ=255)                                                                         cgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                                          >  1:722757/1‑124 (MQ=255)                                                                         cgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                                          <  2:722757/124‑1 (MQ=255)                                                                                cGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTa                                                    <  1:12318/139‑1 (MQ=255)                                                                                   aTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAc                                                                                    <  2:351268/104‑1 (MQ=255)                                                                                   aTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAc                                                                                    >  1:351268/1‑104 (MQ=255)                                                                                                  cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAgcg                                                                                                                         <  2:337606/52‑1 (MQ=255)                                                                                                  cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAgcg                                                                                                                         >  1:337606/1‑52 (MQ=255)                                                                                                  cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTa                                                    <  1:109412/121‑1 (MQ=255)                                                                                                  cgcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTa                                                    >  2:109412/1‑121 (MQ=255)                                                                                                     aTCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGAt                               <  2:58587/139‑1 (MQ=255)                                                                                                                    cAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                          <  2:1202249/49‑1 (MQ=255)                                                                                                                    cAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                          >  1:1202249/1‑49 (MQ=255)                                                                                                                           gACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                              >  1:603787/1‑70 (MQ=255)                                                                                                                           gACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                              <  2:603787/70‑1 (MQ=255)                                                                                                                           gACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                          <  2:84004/106‑1 (MQ=255)                                                                                                                           gACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                          >  1:84004/1‑106 (MQ=255)                                                                                                                            aCGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                              >  1:1458645/1‑69 (MQ=255)                                                                                                                            aCGCTCATACAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                              <  2:1458645/69‑1 (MQ=255)                                                                                                                             cGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                          <  1:526551/40‑1 (MQ=255)                                                                                                                             cGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                          >  2:526551/1‑40 (MQ=255)                                                                                                                                  aTTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCTCCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGaaa  >  2:1441571/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     TCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAA  >  NC_000913/2511333‑2511600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑