Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 247,461 G→A *250* (TAG→TAA yafL → putative lipoprotein and C40 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913247,4610GA100.0% 136.5 / NA 45*250* (TAG→TAAyafLputative lipoprotein and C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/23);  total (22/23)

TTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTC  >  NC_000913/247330‑247588
                                                                                                                                   |                                                                                                                               
ttGGGCGATGGGGAATTTTTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGTCGGAAGAGACGATTTTGTAAGACgata                                                                                                                          <  2:916070/139‑4 (MQ=255)
               tttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTAcgccgc                                                                                                          >  1:1350601/1‑139 (MQ=255)
                ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                                         <  2:688372/139‑1 (MQ=255)
                  aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATc                                                                                                       >  2:1202239/1‑139 (MQ=255)
                      aGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGCCGGAGGAGACGATTTTGTAAGACGATAAGGCGTTTACGCTGCATCCGGc                                                                                                   <  2:1016563/139‑1 (MQ=255)
                          gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGtt                                                                                               >  1:173643/1‑139 (MQ=255)
                                     gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                    >  1:1505928/1‑139 (MQ=255)
                                                cGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGAc                                                                         >  1:302538/1‑139 (MQ=255)
                                                        ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                          <  1:1065836/114‑1 (MQ=255)
                                                        ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                          >  2:1065836/1‑114 (MQ=255)
                                                         cGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                <  2:302538/139‑1 (MQ=255)
                                                             tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                            >  1:488332/1‑139 (MQ=255)
                                                                 cGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCa                                                        <  1:1202239/139‑1 (MQ=255)
                                                                  gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACg                                                                        <  2:1533894/122‑1 (MQ=255)
                                                                  gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACg                                                                        >  1:1533894/1‑122 (MQ=255)
                                                                         tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                >  2:690793/1‑139 (MQ=255)
                                                                              cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                           <  1:958723/139‑1 (MQ=255)
                                                                               aGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcg                                          <  2:39137/139‑1 (MQ=255)
                                                                                ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                             <  2:916659/103‑1 (MQ=255)
                                                                                ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                             >  1:916659/1‑103 (MQ=255)
                                                                                ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                         >  2:1103661/1‑139 (MQ=255)
                                                                                 gACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACgat                                                                                                                           <  2:785684/57‑3 (MQ=255)
                                                                                 gACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACgat                                                                                                                           >  1:785684/1‑55 (MQ=255)
                                                                                  aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                            <  1:746631/86‑1 (MQ=255)
                                                                                  aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                   <  2:100621/111‑1 (MQ=255)
                                                                                  aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                   >  1:100621/1‑111 (MQ=255)
                                                                                  aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                               >  2:474723/1‑115 (MQ=255)
                                                                                  aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                               <  1:474723/115‑1 (MQ=255)
                                                                                  aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATACGGCAGTTGTa                                                                                            >  2:746631/1‑86 (MQ=38)
                                                                                             ggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAg                            >  1:1147776/1‑139 (MQ=255)
                                                                                             ggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAg                            <  2:1505928/139‑1 (MQ=255)
                                                                                                 cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                                         >  2:1347676/1‑58 (MQ=37)
                                                                                                 cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCa                                                                                                         <  1:1347676/58‑1 (MQ=37)
                                                                                                 cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                 >  1:1488183/1‑66 (MQ=25)
                                                                                                 cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                 <  2:1488183/66‑1 (MQ=25)
                                                                                                 cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                        >  1:867488/1‑139 (MQ=255)
                                                                                                  gcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                          <  1:1084541/72‑1 (MQ=37)
                                                                                                  gcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGAGTTTACGCCGCATCCGGCAGTTGTACg                                                                                          >  2:1084541/1‑72 (MQ=37)
                                                                                                      aGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTTCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                   <  1:836221/139‑1 (MQ=37)
                                                                                                      aGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                   <  2:173643/139‑1 (MQ=255)
                                                                                                      aGGATTTTGACGGAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                   <  2:867488/139‑1 (MQ=255)
                                                                                                                  gAAGGGTCGATTTTGTAAGACGATAAGGCGTTGAGGCCGCAACCGGCAGTTGTACGCAGCTGCCCAATGCGCCGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGGGAGCGGCTTTCCCGATATAAgaca       <  2:94582/139‑1 (MQ=21)
                                                                                                                  gAAGAGACGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca       >  1:852207/1‑139 (MQ=255)
                                                                                                                       gaCGATTTTGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTc  <  2:488332/139‑1 (MQ=255)
                                                                                                                             tttGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                <  2:1288092/71‑1 (MQ=17)
                                                                                                                             tttGTAAGACGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                >  1:1288092/1‑71 (MQ=17)
                                                                                                                                   |                                                                                                                               
TTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTC  >  NC_000913/247330‑247588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: