Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,723,065 G→A A113A (GCG→GCA)  pssA → phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,723,0650GA100.0% 205.0 / NA 63A113A (GCG→GCA) pssAphosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/31);  total (32/31)

GTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGT  >  NC_000913/2722936‑2723199
                                                                                                                                 |                                                                                                                                      
gTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAAt                                                                                                                               <  2:689975/139‑1 (MQ=255)
     aTGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGTGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATc                                                                                                                              >  1:510748/1‑135 (MQ=255)
     aTGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGTGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATc                                                                                                                              <  2:510748/135‑1 (MQ=255)
          gCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAg                                                                                                                     <  1:1477533/139‑1 (MQ=255)
            tAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGAt                                                                                                                   >  1:112911/1‑139 (MQ=255)
              aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt                                                                                                                 >  1:962477/1‑139 (MQ=255)
                    aGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGt                                                                                                           >  1:994682/1‑139 (MQ=255)
                        tCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTAt                                                                                                       >  2:238428/1‑139 (MQ=255)
                             aaCTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGt                                                                                                  <  1:1253219/139‑1 (MQ=255)
                                   aTGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaat                                                                                            <  1:238428/139‑1 (MQ=255)
                                            tGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCg                                                                                   >  1:893755/1‑139 (MQ=255)
                                              cTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTg                                                                                 <  2:994682/139‑1 (MQ=255)
                                                       tGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTg                                                                        <  2:498743/139‑1 (MQ=255)
                                                                        tGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCggg                                                                                                                          >  2:809353/1‑72 (MQ=255)
                                                                        tGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCggg                                                                                                                          <  1:809353/72‑1 (MQ=255)
                                                                         ggACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCt                                                                          >  2:605479/1‑119 (MQ=255)
                                                                         ggACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCt                                                                          <  1:605479/119‑1 (MQ=255)
                                                                         ggACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAg                                                      >  1:934011/1‑139 (MQ=255)
                                                                         ggACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAg                                                      >  2:995894/1‑139 (MQ=255)
                                                                                ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCca                                                                                              <  1:759137/92‑1 (MQ=255)
                                                                                ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCca                                                                                              >  2:759137/1‑92 (MQ=255)
                                                                                ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCt                                                                 >  1:663069/1‑121 (MQ=255)
                                                                                ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCt                                                                 <  2:663069/121‑1 (MQ=255)
                                                                                     gcTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTg                                                                        >  2:948823/1‑109 (MQ=255)
                                                                                     gcTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTg                                                                        <  1:948823/109‑1 (MQ=255)
                                                                                      cTGGGGCATCTTACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATcga                                         <  1:995894/139‑1 (MQ=255)
                                                                                         cGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATcgacga                                      >  2:610184/1‑139 (MQ=255)
                                                                                           gCATCTAACACTATCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCCGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATa                                    <  1:1479251/139‑1 (MQ=255)
                                                                                           gCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTatcatc                                           <  1:184453/132‑1 (MQ=255)
                                                                                           gCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTatcatc                                           >  2:184453/1‑132 (MQ=255)
                                                                                              tCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGc                                                               <  1:904017/109‑1 (MQ=255)
                                                                                              tCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGc                                                               >  2:904017/1‑109 (MQ=255)
                                                                                                      tAACGCTGACTGGTACTGCTGCATGGCACAGGAAAATc                                                                                                                              >  1:1023538/1‑38 (MQ=37)
                                                                                                      tAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATc                                                                                                                              <  2:1023538/38‑1 (MQ=255)
                                                                                                        aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg                       >  2:587116/1‑139 (MQ=255)
                                                                                                             tggtactgccgCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTg                  <  2:1305086/129‑1 (MQ=255)
                                                                                                             gacaagTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATTGCGTACTTTATAGCGGTg                  <  1:1013062/134‑1 (MQ=255)
                                                                                                              aCTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGtt                                                                   <  1:248639/89‑1 (MQ=255)
                                                                                                              aCTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGtt                                                                   >  2:248639/1‑89 (MQ=255)
                                                                                                              aCTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTa                                                >  2:524271/1‑108 (MQ=255)
                                                                                                              aCTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTa                                                <  1:524271/108‑1 (MQ=255)
                                                                                                              aCTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGc                 >  1:722427/1‑139 (MQ=255)
                                                                                                                  gTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTc                                                                                                               >  2:459716/1‑41 (MQ=255)
                                                                                                                  gTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTc                                                                                                               <  1:459716/41‑1 (MQ=255)
                                                                                                                       gCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGaa        <  2:386761/139‑1 (MQ=255)
                                                                                                                       gCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGACCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGaa        <  2:893755/139‑1 (MQ=255)
                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATg                                                                                                      <  2:38231/44‑1 (MQ=255)
                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATg                                                                                                      >  1:38231/1‑44 (MQ=255)
                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                                 >  2:1119809/1‑49 (MQ=255)
                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                                 <  1:1119809/49‑1 (MQ=255)
                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCtt                                                  >  2:142048/1‑96 (MQ=255)
                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCtt                                                  <  1:142048/96‑1 (MQ=255)
                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAAc       >  2:1326887/1‑139 (MQ=255)
                                                                                                                            aTGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                                 <  1:488771/45‑1 (MQ=255)
                                                                                                                            aTGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                                 >  2:488771/1‑45 (MQ=255)
                                                                                                                            aTGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGc                                  <  2:807861/108‑1 (MQ=255)
                                                                                                                            aTGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGc                                  >  1:807861/1‑108 (MQ=255)
                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTg                  <  1:1119134/123‑1 (MQ=255)
                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGaa        >  1:770072/1‑133 (MQ=255)
                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGaa        <  2:770072/133‑1 (MQ=255)
                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGt  >  2:1302182/1‑139 (MQ=255)
                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGACGTTCCAATCAATAATCGTAAAGCCCTTGGTGTTCTGCACTTTAGAGGCTTTATCATCGACGATAGCGTACTTTAAAGCGGTGCCGGCCTGAAcctgtc  >  2:1013062/1‑134 (MQ=255)
                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCAGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTg                  >  2:1119134/1‑123 (MQ=255)
                                                                                                                                 |                                                                                                                                      
GTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGT  >  NC_000913/2722936‑2723199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑