Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,146	C→T	A158V (GCG→GTG)	csiR →	transcriptional repressor of csiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,146	0	C	T	100.0%	116.8 / NA	37	A158V (GCG→GTG)	csiR	transcriptional repressor of csiD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/19);  total (18/19)

TGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGC  >  NC_000913/2796022‑2796262                                                                                                                             |                                                                                                                     tgtgACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTggtggc                                                                                                                   >  2:817942/1‑128 (MQ=255) tgtgACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTggtggc                                                                                                                   <  1:817942/128‑1 (MQ=255)  gtgACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGTGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGtt                                                                                                              >  2:666444/1‑132 (MQ=255)  gtgACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGTGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGtt                                                                                                              <  1:666444/132‑1 (MQ=255)  gtgACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGAtt                                                                                                       >  2:146944/1‑139 (MQ=255)                     gCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACg                                                                                    <  1:146944/139‑1 (MQ=255)                      cTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTTTCTGGCTGCGGCGAACgg                                                                                   >  1:475987/1‑139 (MQ=255)                                   ttccttcttatcaGGTTTTTAATACGGGAATTGTGGGCGGCTGGCTTTCTTCGTATTTGCTGCAAACGCGTGAACGGTTGTTCGATCTGGTGGAGCGTTATAGATTTATCTGGATGCGGAGAACGGTGCATTCGGTGGa                                                                      <  2:475987/130‑1 (MQ=255)                                        cGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGTTGCGGCGAACGGTGCTTTCGGTGGAAATGc                                                                 >  1:136696/1‑139 (MQ=255)                                          tcatcaGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTg                                                               <  2:1387419/139‑1 (MQ=255)                                                    tttCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAac                                                     >  2:1141823/1‑139 (MQ=255)                                                       cATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACg                                                  >  2:1374590/1‑139 (MQ=255)                                                        aTACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTAt                                                                                                            >  1:1104117/1‑80 (MQ=255)                                                        aTACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTAt                                                                                                            <  2:1104117/80‑1 (MQ=255)                                                          aCGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATc                                               <  1:1225547/139‑1 (MQ=255)                                                             gCAATTGTGGCGGGCTGTGGTTCTCACTATTTGTTGCAAATTCGTGAACGGTTGTTTGATCTGGTGGTGCGTTATCGTTTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAGATGCTTGAGGGTGAATACGTTctac                                            >  2:349249/1‑136 (MQ=255)                                                               aaTTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCAc                                          <  1:1374590/139‑1 (MQ=255)                                                                    tgGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGAc                                     <  2:856135/139‑1 (MQ=255)                                                                          gCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCTCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGAc                               <  1:816979/139‑1 (MQ=255)                                                                           cTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATGTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGAt                                                <  1:1535302/121‑1 (MQ=255)                                                                           cTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATGTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGAt                                                >  2:1535302/1‑121 (MQ=255)                                                                                     cACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTgcg                                                                                           >  1:1452986/1‑68 (MQ=255)                                                                                     cACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTgcg                                                                                           <  2:1452986/68‑1 (MQ=255)                                                                                      aCTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGTTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACt                   <  2:136696/139‑1 (MQ=255)                                                                                      aCTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACt                   <  2:66273/139‑1 (MQ=255)                                                                                          tttGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGa                                >  2:1336896/1‑122 (MQ=255)                                                                                          tttGCTGCAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGa                                <  1:1336896/122‑1 (MQ=255)                                                                                             gctgcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTgg                                                                                                >  1:745240/1‑55 (MQ=255)                                                                                             gctgcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTgg                                                                                                <  2:745240/55‑1 (MQ=255)                                                                                                gcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt         >  1:1042856/1‑139 (MQ=255)                                                                                                gcAAATGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGAt         >  2:159211/1‑139 (MQ=255)                                                                                                  aaaTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGAt                                                >  2:509648/1‑98 (MQ=255)                                                                                                  aaaTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGAt                                                <  1:509648/98‑1 (MQ=255)                                                                                                    aTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTAt                                                                                                    <  2:469152/44‑1 (MQ=255)                                                                                                    aTGCGTGAACGGTTGTTTGATCTGGTGGCGCGTTATCGATTTAt                                                                                                    >  1:469152/1‑44 (MQ=255)                                                                                                                ttgttTGATCTGGTGGCGCGTTATCGATTTATCTGGCt                                                                                              <  1:904969/38‑1 (MQ=255)                                                                                                                ttgttTGATCTGGTGGCGCGTTATCGATTTATCTGGCt                                                                                              >  2:904969/1‑38 (MQ=255)                                                                                                                        tCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGaa                                                                                      >  1:836488/1‑38 (MQ=255)                                                                                                                        tCTGGTGGCGCGTTATCGATTTATCTGGCTGCGGCGaa                                                                                      <  2:836488/38‑1 (MQ=255)                                                                                                                          tggtggCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTgg                                                                       <  2:3647/51‑1 (MQ=255)                                                                                                                          tggtggCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACcgcgc  <  2:417493/120‑1 (MQ=255)                                                                                                                             |                                                                                                                     TGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTTTGATCTGGCGGCGCGTTATCGATTTATCTGGCTGCGGCGAACGGTGCTTTCGGTGGAAATGCTGGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGC  >  NC_000913/2796022‑2796262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑