Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,814,218	C→T	*172* (TAG→TAA)	luxS ←	S‑ribosylhomocysteine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,814,218	0	C	T	100.0%	128.0 / NA	41	*172* (TAG→TAA)	luxS	S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/22);  total (19/22)

GTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGC  >  NC_000913/2814098‑2814348                                                                                                                         |                                                                                                                                   gtgtGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGc                                                                                                                  >  1:1325957/1‑139 (MQ=255)     ggTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACt                                                                                                              <  2:12269/139‑1 (MQ=255)           cTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCtt                                                                                                        >  2:472172/1‑139 (MQ=255)                ccGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                                   <  1:472172/139‑1 (MQ=255)                   ccATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACt                                                                                                              >  1:1159369/1‑125 (MQ=255)                   ccATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACt                                                                                                              <  2:1159369/125‑1 (MQ=255)                                     ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTg                                                                              <  2:798603/139‑1 (MQ=255)                                          aGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                                   <  1:583034/113‑1 (MQ=255)                                          aGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                                   >  2:583034/1‑113 (MQ=255)                                               gTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTa                                                                    <  1:324282/139‑1 (MQ=255)                                                    cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTAATGCGTACGTCa                                                               >  2:915502/1‑139 (MQ=255)                                                      cTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAAATGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACg                                                             <  1:915502/139‑1 (MQ=255)                                                          gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATAGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                                   >  1:1431004/1‑97 (MQ=255)                                                          gTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATAGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                                   <  2:1431004/97‑1 (MQ=255)                                                                   aGCCAGTTCATTTGCACTGGCTTTTTTCCATTAATTGTGAAGATAGTTAACTGCTTAGGTGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                                >  2:283754/1‑139 (MQ=255)                                                                        gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                 >  1:292046/1‑133 (MQ=255)                                                                        gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                 <  2:292046/133‑1 (MQ=255)                                                                          tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCa                                                                                                                          >  1:1117570/1‑58 (MQ=255)                                                                          tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCa                                                                                                                          <  2:1117570/58‑1 (MQ=255)                                                                          tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTCCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCaa                                         >  1:1265227/1‑139 (MQ=255)                                                                                 aaCTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAc                                               >  2:876028/1‑126 (MQ=255)                                                                                 aaCTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAc                                               <  1:876028/126‑1 (MQ=255)                                                                                   cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTgcg                                >  2:396714/1‑139 (MQ=255)                                                                                   cTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTgcg                                >  1:1445727/1‑139 (MQ=255)                                                                                       cTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTc                            >  2:1514729/1‑139 (MQ=255)                                                                                            ttCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCa                       <  2:1325957/139‑1 (MQ=255)                                                                                                attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                                                                                                <  2:1300267/62‑1 (MQ=255)                                                                                                attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                                                                                                >  1:1300267/1‑62 (MQ=255)                                                                                                attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg                                                                                    >  2:447940/1‑74 (MQ=255)                                                                                                attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCg                                                                                    <  1:447940/74‑1 (MQ=255)                                                                                                attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATg                                                                        >  2:259146/1‑86 (MQ=255)                                                                                                attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATg                                                                        <  1:259146/86‑1 (MQ=255)                                                                                                attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGa                   <  2:451216/139‑1 (MQ=255)                                                                                                attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGa                   >  2:54295/1‑139 (MQ=255)                                                                                                 ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg                  <  1:472080/139‑1 (MQ=255)                                                                                                 ttaattGTGAAGATAGTTTACTGATTAGATGTGCAGTCCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAg                  <  2:1265227/139‑1 (MQ=255)                                                                                                    attGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACg                                                             <  2:1475871/93‑1 (MQ=255)                                                                                                    attGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACg                                                             >  1:1475871/1‑93 (MQ=255)                                                                                                           aGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGt        <  2:539694/139‑1 (MQ=255)                                                                                                           aGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGt        <  1:1514729/139‑1 (MQ=255)                                                                                                                 tttACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGc  <  1:396714/139‑1 (MQ=255)                                                                                                                         |                                                                                                                                   GTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGC  >  NC_000913/2814098‑2814348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑