Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,563	G→A	R354H (CGT→CAT)	proA →	gamma‑glutamylphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,563	0	G	A	100.0%	259.7 / NA	80	R354H (CGT→CAT)	proA	gamma‑glutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/38);  total (42/38)

AGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGGG  >  NC_000913/262437‑262695                                                                                                                                 |                                                                                                                                     aGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGa                                                                                                                          >  1:1353462/1‑139 (MQ=255)     tCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTg                                                                                                                      <  1:268326/139‑1 (MQ=255)             tAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTc                                                                                                              <  1:1290185/139‑1 (MQ=255)               gATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            <  2:756543/139‑1 (MQ=255)                 tttGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCt                                                                                                          >  1:1505343/1‑139 (MQ=255)                   tGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCt                                                                                                          >  1:255728/1‑139 (MQ=255)                     aaCGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGttt                                                                                                      >  1:1419394/1‑139 (MQ=255)                     aaCGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGttt                                                                                                      >  1:651383/1‑139 (MQ=255)                     aaCGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGttt                                                                                                      >  1:1284981/1‑139 (MQ=255)                          cAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGtt                                                                                                 >  2:1303096/1‑139 (MQ=255)                                    aGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTa                                                                                       <  1:1303096/139‑1 (MQ=255)                                    aGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTa                                                                                       <  2:651383/139‑1 (MQ=255)                                                   gCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                              >  1:813951/1‑85 (MQ=255)                                                   gCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                              <  2:813951/85‑1 (MQ=255)                                                   gCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTa                                                                                       >  2:1379899/1‑124 (MQ=255)                                                   gCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTa                                                                                       <  1:1379899/124‑1 (MQ=255)                                                   gCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcg                                                                        >  2:1209118/1‑139 (MQ=255)                                                   gCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcg                                                                        >  2:1500067/1‑139 (MQ=255)                                                   gCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcg                                                                        >  2:281906/1‑139 (MQ=255)                                                    ccATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcgg                                                                       <  2:539280/139‑1 (MQ=255)                                                             atatTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGc                                                                                                           <  2:704859/94‑1 (MQ=255)                                                             atatTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGc                                                                                                           >  1:704859/1‑94 (MQ=255)                                                              tatTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGttt                                                             >  1:242659/1‑139 (MQ=255)                                                                   gTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                              >  2:307656/1‑69 (MQ=255)                                                                   gTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                              <  1:307656/69‑1 (MQ=255)                                                                   gTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCt                                                        >  1:1013228/1‑139 (MQ=255)                                                                    tGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTg                                                       >  1:1104470/1‑139 (MQ=255)                                                                     gA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTgg                                                      >  1:1282374/1‑139 (MQ=255)                                                                          cacGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTg                                                   >  2:94165/1‑139 (MQ=255)                                                                           acGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGc                                                  <  2:1505343/139‑1 (MQ=255)                                                                             ggCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            >  2:1406378/1‑79 (MQ=255)                                                                             ggCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            <  1:1406378/79‑1 (MQ=255)                                                                             ggCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            >  2:555983/1‑79 (MQ=255)                                                                             ggCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            <  1:555983/79‑1 (MQ=255)                                                                             ggCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCgg                                                >  2:931547/1‑139 (MQ=255)                                                                               cacacaACACTCCGATGCGATCCTGACCCGCGATATGCGGAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGaa                                              <  2:1353462/139‑1 (MQ=255)                                                                                acacaACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAg                                             <  2:1284981/139‑1 (MQ=255)                                                                                     acacTCCGATGAGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcg                                                                        >  2:636663/1‑107 (MQ=255)                                                                                     acacTCCGATGAGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcg                                                                        <  1:636663/107‑1 (MQ=255)                                                                                        cTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTa                                     <  1:94165/139‑1 (MQ=255)                                                                                         tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                              <  1:743576/49‑1 (MQ=255)                                                                                         tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                              >  2:743576/1‑49 (MQ=255)                                                                                         tCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTaa                                    >  2:1466551/1‑139 (MQ=255)                                                                                             aTGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                             <  1:1223868/46‑1 (MQ=255)                                                                                             aTGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                             >  2:1223868/1‑46 (MQ=255)                                                                                             aTGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                          >  1:1332194/1‑113 (MQ=255)                                                                                             aTGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                          <  2:1332194/113‑1 (MQ=255)                                                                                              tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                             >  1:574267/1‑45 (MQ=255)                                                                                              tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTaa                                                                                                                             <  2:574267/45‑1 (MQ=255)                                                                                              tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTc                                                                                                              <  1:1383830/60‑1 (MQ=255)                                                                                              tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTc                                                                                                              >  2:1383830/1‑60 (MQ=255)                                                                                              tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggcgg                                                                    <  2:14907/102‑1 (MQ=255)                                                                                              tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAcggcgg                                                                    >  1:14907/1‑102 (MQ=255)                                                                                              tGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGACGGTAAGcaca                               >  1:1164463/1‑139 (MQ=255)                                                                                                cGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                          >  1:1058754/1‑110 (MQ=255)                                                                                                cGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                          <  2:1058754/110‑1 (MQ=255)                                                                                                  aTCGTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGa                                                                          <  1:972510/92‑1 (MQ=255)                                                                                                  aTCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACg                                                                                                                           >  2:1375811/1‑43 (MQ=255)                                                                                                  aTCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACg                                                                                                                           <  1:1375811/43‑1 (MQ=255)                                                                                                  aTCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGa                                                                          >  2:972510/1‑92 (MQ=255)                                                                                                   tCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            >  2:1019524/1‑57 (MQ=255)                                                                                                   tCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            <  1:1019524/57‑1 (MQ=255)                                                                                                       gACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                          <  2:252527/103‑1 (MQ=255)                                                                                                       gACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                          >  1:252527/1‑103 (MQ=255)                                                                                                         cccGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCa                    <  1:141452/139‑1 (MQ=255)                                                                                                         cccGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGAGGATTCGTCCGCTGTTTACGTTAACGGCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCa                    <  1:931547/139‑1 (MQ=255)                                                                                                             cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            >  2:999722/1‑47 (MQ=255)                                                                                                             cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                            <  1:999722/47‑1 (MQ=255)                                                                                                             cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGtt                                                                                 >  2:1360887/1‑74 (MQ=255)                                                                                                             cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGtt                                                                                 <  1:1360887/74‑1 (MQ=255)                                                                                                             cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcg                >  2:981870/1‑139 (MQ=255)                                                                                                               atatGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcgcg              <  2:1458325/139‑1 (MQ=255)                                                                                                               atatGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcgcg              >  1:1458325/1‑139 (MQ=255)                                                                                                               atatGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcgcg              <  1:115844/139‑1 (MQ=255)                                                                                                                  tGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTgg           <  2:242659/139‑1 (MQ=255)                                                                                                                    cgcAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGcc         >  1:1138943/1‑139 (MQ=255)                                                                                                                         cGCCCAGCAATTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATg    <  1:981870/139‑1 (MQ=255)                                                                                                                          gCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACaaa                           >  2:42997/1‑115 (MQ=255)                                                                                                                          gCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACaaa                           <  1:42997/115‑1 (MQ=255)                                                                                                                           cccAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATggg  <  2:255728/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     AGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGA‑‑ACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGGG  >  NC_000913/262437‑262695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑