Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,979,943	C→T	A308V (GCT→GTT)  *231* (TAG→TAA)	lysR → ygeA ←	transcriptional activator of lysA, autorepressor Asp/Glu_racemase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,979,943	0	C	T	100.0%	104.5 / NA	34	A308V (GCT→GTT)  *231* (TAG→TAA)	lysR ygeA	transcriptional activator of lysA, autorepressor Asp/Glu_racemase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

GCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTGCG  >  NC_000913/2979810‑2980069                                                                                                                                      |                                                                                                                               ccGGTTCCGTTCACCGTCAGCCTGATCCGCCGCCTGCACCGCCCGCCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGGTACCGAAACTGGTCCCTTCTCTTGATGCTATTTTGTCGTGAGTTACGa                                                                                                                           <  2:309730/138‑1 (MQ=255)  cGGTTCCGTTCACCGTAAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTCTTTTGTCGTCAGTTACGAc                                                                                                                          >  1:1075575/1‑139 (MQ=255)     ttCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGc                                                                                                                       >  1:761326/1‑139 (MQ=255)                  cAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAg                                                                                                          <  2:157512/139‑1 (MQ=255)                         aTCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTc                                                                                                   >  2:420553/1‑139 (MQ=255)                         aTCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTc                                                                                                   >  1:117381/1‑139 (MQ=255)                                   tGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATc                                                                                         >  2:825747/1‑139 (MQ=255)                                       ccGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgccg                                                                                     <  2:117381/139‑1 (MQ=255)                                       ccGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTAACGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTGAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATcgccg                                                                                     <  2:761326/139‑1 (MQ=255)                                         gCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCcgcg                                                                                   <  1:136721/139‑1 (MQ=255)                                                  caGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAac                                                                          <  1:171271/139‑1 (MQ=255)                                                     cgcTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAAcaca                                                                       >  2:650303/1‑139 (MQ=255)                                                       cTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAgg                                                                     >  2:1177603/1‑139 (MQ=255)                                                        tGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt                                                                                          >  1:296525/1‑117 (MQ=255)                                                        tGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt                                                                                          <  2:296525/117‑1 (MQ=255)                                                                   tttAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTgcg                                                         <  1:1177603/139‑1 (MQ=255)                                                                     tAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTAc                                                                                                                             <  1:1056451/69‑1 (MQ=255)                                                                     tAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTAc                                                                                                                             >  2:1056451/1‑69 (MQ=255)                                                                          gacATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCcgcg                                                                                   >  1:1389011/3‑106 (MQ=255)                                                                          gacATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCcgcg                                                                                   <  2:1389011/104‑1 (MQ=255)                                                                                   aaGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGt                                         <  1:1153189/139‑1 (MQ=255)                                                                                         ggTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCa                                   >  1:1420892/1‑139 (MQ=255)                                                                                          gTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCaa                                  >  2:1438516/1‑139 (MQ=255)                                                                                                       gTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATc                                                                                                      <  2:1257142/58‑1 (MQ=255)                                                                                                       gTCACTTCTCTTGACGCTATTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATc                                                                                                      >  1:1257142/1‑58 (MQ=255)                                                                                                                         aTTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGct                                                       <  1:674247/87‑1 (MQ=255)                                                                                                                         aTTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGct                                                       >  2:674247/1‑87 (MQ=255)                                                                                                                         aTTTTGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTgc   >  2:351334/1‑139 (MQ=255)                                                                                                                          ttttGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt                                                                                          <  1:823992/51‑1 (MQ=255)                                                                                                                          ttttGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGAt                                                                                          >  2:823992/1‑51 (MQ=255)                                                                                                                          ttttGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTgcg  <  1:650303/139‑1 (MQ=255)                                                                                                                          ttttGTCGTCAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTgcg  <  1:1438516/139‑1 (MQ=255)                                                                                                                               tcgtcAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTgcgc                                                        <  2:1382010/80‑1 (MQ=255)                                                                                                                               tcgtcAGTTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTgcgc                                                        >  1:1382010/1‑80 (MQ=255)                                                                                                                                      |                                                                                                                               GCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTCTTGACGCTATTTTGTCGTCAGCTACGACAGCATAAAAGCGACAGCATCCTCGGCATGGATCGCCGCGGTATCAAACACAGGCAAAACACTGCGCTCTTCTGGCACCAGTAAACCAATTTCTGTGCAGCCAAAAATGACGCCCTGTGCG  >  NC_000913/2979810‑2980069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑