Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,993,856	G→A	*73* (TAG→TAA)	ygeI →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,993,856	0	G	A	100.0%	120.2 / NA	38	*73* (TAG→TAA)	ygeI	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/18);  total (20/18)

ACATCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATAT  >  NC_000913/2993726‑2993987                                                                                                                                   |                                                                                                                                    acaTCATCACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCCACATGGATGGCGCATTTTTAAAATGATATGTATTCAGAAGCTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGa                                                                                                                             <  2:1384993/139‑1 (MQ=255)       acacCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaat                                                                                                                       <  1:400504/139‑1 (MQ=255)        cacCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCaaata                                                                                                                      >  1:1066814/1‑139 (MQ=255)              tGCTACTTATACAGATCAAATACAAGCATTTATAGTTTCAACATGTATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCGTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCAt                                                                                                                <  2:947828/139‑1 (MQ=255)                cTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATaa                                                                                                              >  2:131655/1‑139 (MQ=255)                   cTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACga                                                                                                           >  1:617406/1‑139 (MQ=255)                     gATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                         <  1:101134/139‑1 (MQ=255)                     gATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                         <  1:131655/139‑1 (MQ=255)                        aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAACTTGAGTGGTAAACGATTGACCAAATAACTCATAACGAGATAc                                                                                                      >  2:611590/1‑139 (MQ=255)                        aCAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATAc                                                                                                      <  2:617406/139‑1 (MQ=255)                           gATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTCTAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                         <  2:1086705/133‑1 (MQ=255)                           gATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACgaga                                                                                                         >  1:1086705/1‑133 (MQ=255)                                  tACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTataata                                                                                            <  2:322135/139‑1 (MQ=255)                                        cATTTATAGTTTCAACATGGATGGTGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGATTGGTAAACGATTGACCaaat                                                                                                                       <  1:136347/106‑1 (MQ=255)                                        cATTTATAGTTTCAACATGGATGGTGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGATTGGTAAACGATTGACCaaat                                                                                                                       >  2:136347/1‑106 (MQ=255)                                                   tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                           >  2:787830/1‑139 (MQ=255)                                                   tCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttca                                                                           >  1:101630/1‑139 (MQ=255)                                                           gatggCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATcc                                                                   >  2:1289089/1‑139 (MQ=255)                                                               gcgcCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTcac                                                               <  2:430045/139‑1 (MQ=255)                                                                     tttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCg                                                                                    >  1:122361/1‑112 (MQ=255)                                                                     tttCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCg                                                                                    <  2:122361/112‑1 (MQ=255)                                                                                       tCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCa                                       >  2:1449508/1‑139 (MQ=255)                                                                                         agaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                         <  1:427335/103‑1 (MQ=255)                                                                                         agaagaTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                         >  2:427335/1‑103 (MQ=255)                                                                                              aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                >  2:846183/1‑139 (MQ=255)                                                                                              aTAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTa                                >  2:1363674/1‑139 (MQ=255)                                                                                               tAATCCTATCTCACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTaa                               <  1:846183/139‑1 (MQ=255)                                                                                                       tctcACCTTACTATAAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCATTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTaa                       >  1:644208/1‑139 (MQ=255)                                                                                                                  tataAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACg            <  1:611590/139‑1 (MQ=255)                                                                                                                  tataAAATTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACg            <  1:929903/139‑1 (MQ=255)                                                                                                                       aaTTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGtt          >  2:438628/1‑136 (MQ=255)                                                                                                                       aaTTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGtt          <  1:438628/136‑1 (MQ=255)                                                                                                                       aaTTGAGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTg       >  2:468270/1‑139 (MQ=255)                                                                                                                            aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                         >  1:635643/1‑68 (MQ=255)                                                                                                                            aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTttcatt                                                                         <  2:635643/68‑1 (MQ=255)                                                                                                                            aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCa              <  2:425793/127‑1 (MQ=255)                                                                                                                            aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCa              >  1:425793/1‑127 (MQ=255)                                                                                                                            aGTGGTAAACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTatat  >  1:49395/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                    ACATCAACACCAGTGCTACTGATACAGATCAAATACAAGCATTTATAGTTTCAACATGGATGGCGCCTTTTCAAAATGATATGTATTCAGAAGATAATCCTATCTCACCTTACTATAAAATTGAGTGGTAGACGATTGACCAAATAAATCATAACGAGATACTTTTATAATAGTATCTCGTTCCTTTCATTCAATCCTCACACATGAAAAAATATGCGCTCACCAAAAGTTAAATTCTTAACCATCTTCACGTTTTGTATAT  >  NC_000913/2993726‑2993987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑