Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,032	G→A	S222S (TCG→TCA)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,032	0	G	A	100.0%	218.0 / NA	68	S222S (TCG→TCA)	uacT	uric acid permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/35);  total (33/35)

ATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCA  >  NC_000913/3031902‑3032162                                                                                                                                   |                                                                                                                                   atGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGgatgat                                                                                                                            >  2:1259622/1‑139 (MQ=255)        tCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGAtgaatgaa                                                                                                                     >  1:266048/1‑139 (MQ=255)        tCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGAtgaatgaa                                                                                                                     <  1:1259622/139‑1 (MQ=255)            gTTTATTTAGGTATCTCTTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATgg                                                                                                                                 <  1:369025/123‑1 (MQ=255)            gTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATgg                                                                                                                                 >  2:369025/1‑123 (MQ=255)             tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCaa                                                                                                                >  1:909644/1‑139 (MQ=255)                  ttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           <  1:600324/139‑1 (MQ=255)                          ctcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      >  1:1014977/1‑136 (MQ=255)                          ctcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      <  2:1014977/136‑1 (MQ=255)                          ctcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTa                                                                                                   >  1:396630/1‑139 (MQ=255)                              tttGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                               <  1:374080/139‑1 (MQ=255)                                     tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATcc                                                                                                         <  1:647209/122‑1 (MQ=255)                                     tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATcc                                                                                                         >  2:647209/1‑122 (MQ=255)                                     tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      <  2:590880/125‑1 (MQ=255)                                     tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      >  1:590880/1‑125 (MQ=255)                                       ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGatgaa                                                                                                                         >  1:937071/1‑104 (MQ=255)                                       ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGatgaa                                                                                                                         <  2:937071/104‑1 (MQ=255)                                               tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATc                                                                                                          >  1:420452/1‑111 (MQ=255)                                               tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATc                                                                                                          <  2:420452/111‑1 (MQ=255)                                               tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                       >  2:1051231/1‑130 (MQ=255)                                               tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                       <  1:1051231/130‑1 (MQ=255)                                                     gCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      <  2:845644/109‑1 (MQ=255)                                                     gCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCggg                                                                                                      >  1:845644/1‑109 (MQ=255)                                                           tACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                  >  2:1451791/1‑139 (MQ=255)                                                                gCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                             >  2:70227/1‑123 (MQ=255)                                                                gCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                             <  1:70227/123‑1 (MQ=255)                                                                gCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTc                                                             >  1:75919/1‑139 (MQ=255)                                                                   aTGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGtt                                                          >  2:1510024/1‑139 (MQ=255)                                                                     gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTg                                                        >  1:177868/1‑139 (MQ=255)                                                                     gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTg                                                        >  1:1402116/1‑139 (MQ=255)                                                                     gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTg                                                        >  1:167334/1‑139 (MQ=255)                                                                      cGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTgg                                                       <  2:396630/139‑1 (MQ=255)                                                                               ttATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGAt                                              <  2:909644/139‑1 (MQ=255)                                                                               ttATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGAt                                              <  1:1510024/139‑1 (MQ=255)                                                                                      cAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                               >  2:1204097/1‑83 (MQ=255)                                                                                      cAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                               <  1:1204097/83‑1 (MQ=255)                                                                                         cGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                               >  1:769190/1‑80 (MQ=255)                                                                                         cGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                               <  2:769190/80‑1 (MQ=255)                                                                                            cGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGtt                                 >  1:921047/1‑139 (MQ=255)                                                                                            cGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGtt                                 >  2:386361/1‑139 (MQ=255)                                                                                             gCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGttt                                <  2:1402116/139‑1 (MQ=255)                                                                                             gCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGttt                                <  2:177868/139‑1 (MQ=255)                                                                                              ccGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                             <  1:291277/93‑1 (MQ=255)                                                                                              ccGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                             >  2:291277/1‑93 (MQ=255)                                                                                              ccGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTc                               <  2:75919/139‑1 (MQ=255)                                                                                              ccGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTc                               >  2:728094/1‑139 (MQ=255)                                                                                                  aTTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCAtt                           <  2:921047/139‑1 (MQ=255)                                                                                                         gggATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCg                                                                                                        <  1:1013386/55‑1 (MQ=255)                                                                                                         gggATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCg                                                                                                        >  2:1013386/1‑55 (MQ=255)                                                                                                         gggATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGt                                                      <  2:1353687/105‑1 (MQ=255)                                                                                                         gggATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGt                                                      >  1:1353687/1‑105 (MQ=255)                                                                                                           gATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           >  1:1375622/1‑50 (MQ=255)                                                                                                           gATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           <  2:1375622/50‑1 (MQ=255)                                                                                                              tGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           >  2:690304/1‑47 (MQ=255)                                                                                                              tGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTAt                                                                                                           <  1:690304/47‑1 (MQ=255)                                                                                                              tGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                     <  2:557711/117‑1 (MQ=255)                                                                                                              tGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                     >  1:557711/1‑117 (MQ=255)                                                                                                               gTATTTGGCTTTTTACTTTCATGGATGATGAATTAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg              <  2:69480/139‑1 (MQ=255)                                                                                                               gTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg              <  1:882952/139‑1 (MQ=255)                                                                                                               gTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg              <  1:662562/139‑1 (MQ=255)                                                                                                                tATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGTGATTGt                                                                         >  2:1235721/1‑79 (MQ=255)                                                                                                                tATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGTGATTGt                                                                         <  1:1235721/79‑1 (MQ=255)                                                                                                                tATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTAACCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGc             >  2:401330/1‑139 (MQ=255)                                                                                                                 aTTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGcc            <  1:1444388/139‑1 (MQ=255)                                                                                                                     ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                     <  1:1099141/110‑1 (MQ=255)                                                                                                                     ggCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTAAGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                     >  2:1099141/1‑110 (MQ=255)                                                                                                                       cTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTa      <  1:728094/139‑1 (MQ=255)                                                                                                                           ttACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatca  <  1:1529483/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                   ATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCA  >  NC_000913/3031902‑3032162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑