Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	291,324	C→T	C8Y (TGT→TAT)	insA ←	IS1 repressor TnpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	291,324	0	C	T	100.0%	64.7 / NA	22	C8Y (TGT→TAT)	insA	IS1 repressor TnpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

CGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGTTG  >  NC_000913/291271‑291458                                                      |                                                                                                                                         cGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACACTAAATCAGTAAGTTGGCAGCATCACCGAAttt                                                     >  1:1317256/1‑139 (MQ=11)  ggCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTc                                                   <  2:452100/138‑1 (MQ=21)  ggCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTc                                                   >  1:452100/1‑138 (MQ=21)   gCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGTAGCATCACCGAATTTTCAg                                                 <  2:1425990/139‑1 (MQ=255)   gCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAg                                                 >  2:647577/1‑139 (MQ=37)      gTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGccc                                              <  1:76027/139‑1 (MQ=255)      gTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGccc                                              >  1:968819/1‑139 (MQ=255)      gTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGccc                                              >  2:1348528/1‑139 (MQ=255)       tGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCg                                             >  2:634390/1‑139 (MQ=255)        gCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCgg                                            <  1:634390/139‑1 (MQ=255)        gCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCgg                                            <  1:647577/139‑1 (MQ=255)                cGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCa                                    <  1:454197/139‑1 (MQ=255)                 gTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCAc                                   >  2:1306158/1‑139 (MQ=255)                       caccacCCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCt                             <  1:1348528/139‑1 (MQ=255)                        accaccCCGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTa                            <  1:295731/139‑1 (MQ=255)                               cGTCAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGaa                     >  2:674096/1‑139 (MQ=255)                                  cAGTAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAatat                  >  1:326896/1‑139 (MQ=255)                                     tAGCTGAACAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTg               <  2:729036/139‑1 (MQ=255)                                           aaCAGGAGGGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGAt         >  1:786550/1‑139 (MQ=255)                                               ggaggGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCa                                    <  2:299009/108‑1 (MQ=255)                                               ggaggGATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCa                                    >  1:299009/1‑108 (MQ=255)                                                  gggATAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGaggttgcgg  >  1:1249721/1‑131 (MQ=255)                                                      |                                                                                                                                         CGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCAT‑‑ACACTAAATCAGTAAGTTGGCAGCATCACCGAATTTTCAGCCCGGATGTTTCACGTACCTATGTCGAATATCTGGCCGATAAGGTTG  >  NC_000913/291271‑291458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑