Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,094,234 T→C T283A (ACA→GCA)  yggR ← putative PilT family AAA+ ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,094,2340TC98.5% 228.2 / NA 66T283A (ACA→GCA) yggRputative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (34/31);  minor base A (1/0);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAA  >  NC_000913/3094109‑3094364
                                                                                                                             |                                                                                                                                  
cccTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAAt                                                                                                                       >  2:22771/1‑139 (MQ=255)
cccTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAAt                                                                                                                       <  1:22771/139‑1 (MQ=255)
                 gCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                                                      <  1:1131252/139‑1 (MQ=255)
                     aTAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                                                  >  1:1062845/1‑139 (MQ=255)
                          tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttc                                                                                                >  2:426927/1‑136 (MQ=255)
                          tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttc                                                                                                <  1:426927/136‑1 (MQ=255)
                              ctgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTa                                                                                         >  2:641827/1‑139 (MQ=255)
                               tgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTAt                                                                                        >  1:401298/1‑139 (MQ=255)
                                gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                                       <  2:401298/139‑1 (MQ=255)
                                   aCGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcc                                                                                                   <  2:713653/124‑1 (MQ=255)
                                   aCGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcc                                                                                                   >  1:713653/1‑124 (MQ=255)
                                        atcatcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                                       <  2:841366/131‑1 (MQ=255)
                                        atcatcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                                       >  1:841366/1‑131 (MQ=255)
                                        atcatcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCa                                                                               >  2:689363/1‑139 (MQ=255)
                                        atcatcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCa                                                                               >  2:656309/1‑139 (MQ=255)
                                                 aCCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                                                      <  2:356180/107‑1 (MQ=255)
                                                 aCCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                                                      >  1:356180/1‑107 (MQ=255)
                                                 aCCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTtgtg                                                                      >  2:97110/1‑139 (MQ=255)
                                                   ctgctgCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTc                                                                                 <  2:1190440/126‑1 (MQ=255)
                                                   ctgctgCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTc                                                                                 >  1:1190440/1‑126 (MQ=255)
                                                      ctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGc                                                                                                                          <  2:593130/82‑1 (MQ=255)
                                                      ctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGc                                                                                                                          >  1:593130/1‑82 (MQ=255)
                                                      ctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCATGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGc                                                                 >  2:46132/1‑139 (MQ=255)
                                                      ctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGc                                                                 >  1:896728/1‑139 (MQ=255)
                                                      ctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGAGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGc                                                                 >  2:353954/1‑139 (MQ=255)
                                                       tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCa                                                                <  1:611985/139‑1 (MQ=255)
                                                        gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttcc                                                                                               <  1:612131/107‑1 (MQ=255)
                                                        gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttcc                                                                                               >  2:612131/1‑107 (MQ=255)
                                                        gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               <  1:421706/139‑1 (MQ=255)
                                                        gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               <  1:46132/139‑1 (MQ=255)
                                                        gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGCTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               <  2:896728/139‑1 (MQ=255)
                                                           cGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGc                                                            <  1:34408/139‑1 (MQ=255)
                                                             gTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAg                                                                                                                           <  1:1106905/74‑1 (MQ=255)
                                                             gTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAg                                                                                                                           >  2:1106905/1‑74 (MQ=255)
                                                                  aaTAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTaaa                                                     <  2:1479872/139‑1 (MQ=255)
                                                                       cATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGATTTTGTGACAGCACTGCCCGTAAACTAcc                                                >  1:1037613/1‑139 (MQ=255)
                                                                        aTGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCt                                               >  2:368161/1‑139 (MQ=255)
                                                                          gCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGc                                             <  1:811614/139‑1 (MQ=255)
                                                                            ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               >  1:1263318/1‑119 (MQ=255)
                                                                            ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               <  2:1263318/119‑1 (MQ=255)
                                                                            ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCa                                           >  1:1460892/1‑139 (MQ=255)
                                                                             gTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAg                                          <  1:37648/139‑1 (MQ=255)
                                                                              tAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGt                                         <  1:656309/139‑1 (MQ=255)
                                                                                 cTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAgc                                                                                                              <  1:59556/67‑1 (MQ=255)
                                                                                 cTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAgc                                                                                                              >  2:59556/1‑67 (MQ=255)
                                                                                 cTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                                       <  1:964448/90‑1 (MQ=255)
                                                                                 cTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                                       >  2:964448/1‑90 (MQ=255)
                                                                                  tggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               <  2:714686/113‑1 (MQ=255)
                                                                                  tggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               >  1:714686/1‑113 (MQ=255)
                                                                                  tggtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGAt                                     >  1:297415/1‑139 (MQ=255)
                                                                                    gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCa                                                                                                                            >  2:196577/1‑50 (MQ=255)
                                                                                    gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCa                                                                                                                            <  1:196577/50‑1 (MQ=255)
                                                                                    gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGtt                                                                                           >  2:689968/1‑83 (MQ=255)
                                                                                    gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGtt                                                                                           <  1:689968/83‑1 (MQ=255)
                                                                                    gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACGGCACTGCCCGTAAACTACCTGCCAGTTGATTa                                   <  2:1037613/139‑1 (MQ=255)
                                                                                            tCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGcc                                                                                                           <  2:835112/59‑1 (MQ=255)
                                                                                            tCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGcc                                                                                                           >  1:835112/1‑59 (MQ=255)
                                                                                            tCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               <  1:293533/103‑1 (MQ=255)
                                                                                            tCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                                               >  2:293533/1‑103 (MQ=255)
                                                                                            tCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACggg                           >  1:923093/1‑139 (MQ=255)
                                                                                                   gcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCtttt                    >  1:93705/1‑139 (MQ=255)
                                                                                                                cccACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGaa       <  2:1062845/139‑1 (MQ=255)
                                                                                                                   aCCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTCTCCACTTCCAGCTTTTGTGACAGAACTGCCCGTACACTCCCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATg    >  2:382087/1‑139 (MQ=255)
                                                                                                                     cgcgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTAt                                                                                        <  1:923683/53‑1 (MQ=255)
                                                                                                                     cgcgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTAt                                                                                        >  2:923683/1‑53 (MQ=255)
                                                                                                                     cgcgGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGaa  >  1:540489/1‑139 (MQ=255)
                                                                                                                          gtgcgtTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                                                  <  1:1374220/38‑1 (MQ=255)
                                                                                                                             |                                                                                                                                  
CCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTCCTGCGCCGGAAATGAA  >  NC_000913/3094109‑3094364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: