Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,140,294 T→C S9G (AGC→GGC)  hybF ← protein involved with the maturation of hydrogenases 1 and 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,2940TC100.0% 87.1 / NA 26S9G (AGC→GGC) hybFprotein involved with the maturation of hydrogenases 1 and 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/15);  total (11/15)

ACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTC  >  NC_000913/3140167‑3140415
                                                                                                                               |                                                                                                                         
aCAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAagag                                                                                                                >  1:1035348/1‑139 (MQ=255)
 cAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                               <  2:292703/139‑1 (MQ=255)
        aaaaCTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCa                                                                                                        <  2:282830/139‑1 (MQ=255)
               aaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCa                                                                                                                     <  1:228102/119‑1 (MQ=255)
               aaaCGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCa                                                                                                                     >  2:228102/1‑119 (MQ=255)
                              tcctcAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGa                                                                                  <  2:1035348/139‑1 (MQ=255)
                                     cGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                              <  1:244629/104‑1 (MQ=255)
                                     cGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                              >  2:244629/1‑104 (MQ=255)
                                       cAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcagc                                                                         <  1:494921/139‑1 (MQ=255)
                                        aGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                              >  1:1295055/1‑101 (MQ=255)
                                        aGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                              <  2:1295055/101‑1 (MQ=255)
                                          gagaGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAgg                                                                      >  2:1038680/1‑139 (MQ=255)
                                               cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                 >  2:1451949/1‑139 (MQ=255)
                                                    cAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                            <  1:1451949/139‑1 (MQ=255)
                                                      aTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCGCTCCTGCGACCAAACAGCAGGGCGCGACGCCCt                                                          <  1:1038680/139‑1 (MQ=255)
                                                      aTTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCt                                                          <  2:41229/139‑1 (MQ=255)
                                                       tttCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTg                                                         <  1:1053852/139‑1 (MQ=255)
                                                                           aCGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTc                                     >  1:323760/1‑139 (MQ=255)
                                                                                   aaCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCAtt                                                                                                  <  1:966357/70‑1 (MQ=255)
                                                                                   aaCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCAtt                                                                                                  >  2:966357/1‑70 (MQ=255)
                                                                                               ctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCa                                                                                                                     <  1:717434/39‑1 (MQ=38)
                                                                                               ctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCa                                                                                                                     >  2:717434/1‑39 (MQ=38)
                                                                                                    ccTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGGAAGGATCATTc            >  1:998996/1‑139 (MQ=255)
                                                                                                       gCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                              <  2:1245875/38‑1 (MQ=255)
                                                                                                       gCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                              >  1:1245875/1‑38 (MQ=255)
                                                                                                              gATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTc  <  2:323760/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                         
ACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTC  >  NC_000913/3140167‑3140415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: