Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	309,358	C→T	*223* (TAG→TAA)	ecpB ←	ECP production pilus chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	309,358	0	C	T	100.0%	121.9 / NA	38	*223* (TAG→TAA)	ecpB	ECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/18);  total (20/18)

ATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCG  >  NC_000913/309223‑309493                                                                                                                                        |                                                                                                                                        aTTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGctatttat                                                                                                                                      >  2:1457567/1‑139 (MQ=255)             cTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg                                                                                                                          <  1:1360152/139‑1 (MQ=255)             cTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATg                                                                                                                          <  1:1457567/139‑1 (MQ=255)                        agcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCAcc                                                                                                               >  2:1430421/1‑139 (MQ=255)                        agcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCAcc                                                                                                               >  1:1536635/1‑139 (MQ=255)                          cgcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCt                                                                                                             >  2:1430898/1‑139 (MQ=255)                             tGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTA‑TTCACGGGAATGAACTTATCACCCTGCCa                                                                                                         <  1:479903/139‑1 (MQ=255)                                                        ccATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg                                                                               <  1:665457/139‑1 (MQ=255)                                                          aTGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa                                                                             >  1:889844/1‑139 (MQ=255)                                                          aTGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa                                                                             <  1:1430898/139‑1 (MQ=255)                                                                             cTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAac                                                          >  2:839735/1‑139 (MQ=255)                                                                                        ggggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                >  2:737/1‑90 (MQ=255)                                                                                        ggggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                <  1:737/90‑1 (MQ=255)                                                                                          ggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc                                             <  1:1430421/139‑1 (MQ=255)                                                                                              aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGc                                         >  1:957253/1‑139 (MQ=255)                                                                                                   tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAGCCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCg                                           <  2:917419/132‑1 (MQ=255)                                                                                                   tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAGCCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCg                                           >  1:917419/1‑132 (MQ=255)                                                                                                    cctAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGAAACCCTTCCTTTGTTATACGCAACGTCCACGCGGGTAAAAACACCCTACTTGCCCGGCATCAgc                                   >  2:920549/3‑138 (MQ=255)                                                                                                          ggCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTaa                             >  2:24917/1‑139 (MQ=255)                                                                                                           gCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAt                            <  1:839735/139‑1 (MQ=255)                                                                                                                     tATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTaca                  <  1:24917/139‑1 (MQ=255)                                                                                                                     tATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTaca                  >  2:1438306/1‑139 (MQ=255)                                                                                                                      aTCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTg                                                                                                    >  2:116084/1‑56 (MQ=255)                                                                                                                      aTCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTg                                                                                                    <  1:116084/56‑1 (MQ=255)                                                                                                                      aTCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                <  1:372465/60‑1 (MQ=255)                                                                                                                      aTCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                >  2:372465/1‑60 (MQ=255)                                                                                                                           tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCTACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgtagta                              >  2:543887/1‑121 (MQ=255)                                                                                                                           tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCTACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgtagta                              <  1:543887/121‑1 (MQ=255)                                                                                                                           tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg                                                                               <  1:412311/72‑1 (MQ=255)                                                                                                                           tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg                                                                               >  2:412311/1‑72 (MQ=255)                                                                                                                           tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc                                                                     <  1:913338/82‑1 (MQ=255)                                                                                                                           tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGc                                              >  1:744161/1‑105 (MQ=255)                                                                                                                           tCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGc                                              <  2:744161/105‑1 (MQ=255)                                                                                                                           tCTGCACAGCTATTTATTTCACGGGAAGGAACCTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc                                                                     >  2:913338/1‑82 (MQ=255)                                                                                                                               cacaGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                                       <  1:102811/108‑1 (MQ=255)                                                                                                                               cacaGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                                       >  2:102811/1‑108 (MQ=255)                                                                                                                                 caGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                <  2:4495/49‑1 (MQ=255)                                                                                                                                 caGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                >  1:4495/1‑49 (MQ=255)                                                                                                                                   gCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTgg    >  2:344642/1‑139 (MQ=255)                                                                                                                                     tatttattTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggcg  <  1:344642/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        ATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCG  >  NC_000913/309223‑309493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑