Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	312,189	C→T	G26G (GGC→GGT)	ykgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,189	0	C	T	100.0%	197.7 / NA	62	G26G (GGC→GGT)	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/32);  total (30/32)

CCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACA  >  NC_000913/312068‑312318                                                                                                                          |                                                                                                                                  ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  <  1:1127550/139‑1 (MQ=255) ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  <  2:199146/139‑1 (MQ=255) ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAAGGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  <  2:1235325/139‑1 (MQ=255)     cAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAAc                                                                                                              <  1:1479856/139‑1 (MQ=255)        aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCAAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAtt                                                                                                           <  2:28490/139‑1 (MQ=255)                caTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAAt                                                                                                   <  2:449639/139‑1 (MQ=255)                        atcTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATAtt                                                                                           <  1:1061447/139‑1 (MQ=255)                           ttAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                              <  1:1289011/101‑1 (MQ=255)                           ttAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGc                                                                                                                              >  2:1289011/1‑101 (MQ=255)                           ttAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCgg                                                                                        >  1:1434514/1‑139 (MQ=255)                             aaCAGTTTGAGTGAAAATGCAGGCTGTGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 >  2:195788/1‑112 (MQ=255)                             aaCAGTTTGAGTGAAAATGCAGGCTGTGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 <  1:195788/112‑1 (MQ=255)                             aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgag                                                                                      >  2:1357670/1‑139 (MQ=255)                                  tttGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCg                                                                                         >  2:438033/1‑131 (MQ=255)                                  tttGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCg                                                                                         <  1:438033/131‑1 (MQ=255)                                  tttGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGaaa                                                                                 >  1:870532/1‑139 (MQ=255)                                    tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTc                                                                                                                         <  1:255133/97‑1 (MQ=255)                                    tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTc                                                                                                                         >  2:255133/1‑97 (MQ=255)                                    tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAtt                                                                                                           >  1:621761/1‑111 (MQ=255)                                    tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAtt                                                                                                           <  2:621761/111‑1 (MQ=255)                                    tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACg                                                                               >  2:1186031/1‑139 (MQ=255)                                    tGAGTGAAAATGCAGGCTGCGCCGCAAA‑GGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 <  1:666784/104‑1 (MQ=255)                                    tGAGTGAAAATGCAGGCTGCGCCGCAAA‑GGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 >  2:666784/1‑104 (MQ=255)                                         gAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAgg                                                                          <  1:1357670/139‑1 (MQ=255)                                                cAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTgg                                                                   >  2:1286802/1‑139 (MQ=255)                                                       cgccgcAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCaaa                                                            >  1:1206583/1‑139 (MQ=255)                                                         ccgcAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 <  1:304221/84‑1 (MQ=255)                                                         ccgcAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTc                                                                                                                 >  2:304221/1‑84 (MQ=255)                                                            cAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       <  1:807053/139‑1 (MQ=255)                                                             aaaGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATg                                                          <  1:71405/135‑1 (MQ=255)                                                             aaaGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATg                                                          >  2:71405/1‑135 (MQ=255)                                                               aGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAAt                                                                                                   <  2:436735/92‑1 (MQ=255)                                                               aGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAAt                                                                                                   >  1:436735/1‑92 (MQ=255)                                                                         cTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  <  1:429428/67‑1 (MQ=255)                                                                         cTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  >  2:429428/1‑67 (MQ=255)                                                                            aGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaac                                                                                                                      >  1:1528896/1‑60 (MQ=255)                                                                            aGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTcaac                                                                                                                      <  2:1528896/60‑1 (MQ=255)                                                                            aGTGGCGTGTTCAGTCTAAAAGAGAGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTc                                                                                          >  1:854606/1‑88 (MQ=255)                                                                            aGTGGCGTGTTCAGTCTAAAAGAGAGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACAGTAAGATAATGGAATATTc                                                                                          <  2:854606/88‑1 (MQ=255)                                                                               ggCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAg                                    <  2:1206583/139‑1 (MQ=255)                                                                                          tCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATg                         >  2:59566/1‑139 (MQ=255)                                                                                              aaaGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGgaa                                                  <  2:564060/110‑1 (MQ=255)                                                                                              aaaGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGgaa                                                  >  1:564060/1‑110 (MQ=255)                                                                                               aaGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  <  2:72629/45‑1 (MQ=255)                                                                                               aaGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGt                                                                                                                  >  1:72629/1‑45 (MQ=255)                                                                                                    ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       >  2:1211802/1‑99 (MQ=255)                                                                                                    ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       <  1:1211802/99‑1 (MQ=255)                                                                                                    ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtat               >  2:181565/1‑139 (MQ=255)                                                                                                    ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtat               >  1:219189/1‑139 (MQ=255)                                                                                                    ggTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtat               >  1:762391/1‑139 (MQ=255)                                                                                                    ggTTGATGCCAGCATAC‑‑GGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaa             >  1:679546/1‑139 (MQ=255)                                                                                                      ttGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTa                                                                                                          <  2:1148112/46‑1 (MQ=255)                                                                                                      ttGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTa                                                                                                          >  1:1148112/1‑46 (MQ=255)                                                                                                         aTGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGga                                                   <  1:433911/98‑1 (MQ=255)                                                                                                         aTGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGga                                                   >  2:433911/1‑98 (MQ=255)                                                                                                            ccAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac       <  1:59566/139‑1 (MQ=255)                                                                                                             cAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACAcc      <  1:181565/139‑1 (MQ=255)                                                                                                              aGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGTTAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGga                                                   <  1:1476338/93‑1 (MQ=255)                                                                                                              aGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGTTAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGga                                                   >  2:1476338/1‑93 (MQ=255)                                                                                                                 aTACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACa  <  2:333281/139‑1 (MQ=255)                                                                                                                     aGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       >  2:957704/1‑82 (MQ=255)                                                                                                                     aGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                       <  1:957704/82‑1 (MQ=255)                                                                                                                          |                                                                                                                                  CCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACA  >  NC_000913/312068‑312318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑