Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,218,365 | C→T | E238K (GAG→AAG) | aer ← | fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,218,365 | 0 | C | T | 98.4% | 195.1 / NA | 63 | E238K (GAG→AAG) | aer | fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component |
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (29/33); total (30/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
TTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAA > NC_000913/3218232‑3218494 | ttcagttcaTCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGc < 1:526754/139‑1 (MQ=255) ttcaTCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGa < 2:1117548/139‑1 (MQ=255) tGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATg > 2:378949/1‑139 (MQ=255) ccTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt > 2:123421/1‑139 (MQ=255) ttCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCaa < 1:572086/139‑1 (MQ=255) cGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTa > 2:97038/1‑139 (MQ=255) gCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCg < 2:41266/139‑1 (MQ=255) aTTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTc < 1:1478344/139‑1 (MQ=255) aTTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTc < 1:216678/139‑1 (MQ=255) aTTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTc < 1:378949/139‑1 (MQ=255) cTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCa < 1:123421/139‑1 (MQ=255) acTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTc < 1:97038/139‑1 (MQ=255) cTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCa < 1:1252302/139‑1 (MQ=255) tGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTtct > 1:595971/1‑122 (MQ=255) tGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTtct < 2:595971/122‑1 (MQ=255) tGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCt < 1:918146/135‑1 (MQ=255) tGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCt > 2:918146/1‑135 (MQ=255) gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCaa < 2:1177904/106‑1 (MQ=255) gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACTTTCTCCGGTCGCCACCTTCAGt < 1:1224290/138‑1 (MQ=255) gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGt > 2:1224290/1‑138 (MQ=255) gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCaa > 1:1177904/1‑106 (MQ=255) ccTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTg < 2:1351551/139‑1 (MQ=255) ggCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGc < 1:437694/84‑1 (MQ=255) ggCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGc > 2:437694/1‑84 (MQ=255) gCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATgg > 1:1254622/1‑139 (MQ=255) cTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATggg < 2:268385/139‑1 (MQ=255) tGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCGCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATgggta < 2:1223450/139‑3 (MQ=255) cGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTc > 2:735694/1‑139 (MQ=255) cGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTc > 2:632833/1‑139 (MQ=255) aaTTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAg < 1:814254/75‑1 (MQ=255) aaTTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAg > 2:814254/1‑75 (MQ=255) aGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAaca < 2:1169951/81‑1 (MQ=255) aGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAaca > 1:1169951/1‑81 (MQ=255) gCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcg < 1:735694/139‑1 (MQ=255) ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcg > 2:628596/1‑138 (MQ=255) ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcg < 1:628596/138‑1 (MQ=255) cGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGTCTg > 2:750815/1‑110 (MQ=255) cGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGTCTg < 1:750815/110‑1 (MQ=255) acaTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTg < 1:1197298/139‑1 (MQ=255) aTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGcc > 2:622352/1‑139 (MQ=255) aTCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGcc > 2:52056/1‑139 (MQ=255) aGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCAtt < 1:632833/139‑1 (MQ=255) gTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATg > 2:1017328/1‑52 (MQ=255) gTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATg < 1:1017328/52‑1 (MQ=255) gTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGc < 1:754601/128‑1 (MQ=255) gTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGc > 2:754601/1‑128 (MQ=255) gTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACa > 2:1314421/1‑139 (MQ=255) cGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTc > 2:957274/1‑67 (MQ=255) cGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTc < 1:957274/67‑1 (MQ=255) cACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCcaa < 2:1254622/139‑1 (MQ=255) cACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCcaa > 1:1459496/1‑139 (MQ=255) cGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCcaaca > 1:916472/1‑139 (MQ=255) cGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCcaaca > 2:115025/1‑139 (MQ=255) tGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATAcc > 2:1369830/1‑139 (MQ=255) aGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcg < 2:1178870/95‑1 (MQ=255) aGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcg > 1:1178870/1‑95 (MQ=255) gCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTaa > 1:418182/1‑139 (MQ=255) ccccAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTAt > 2:46611/1‑87 (MQ=255) ccccAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTAt < 1:46611/87‑1 (MQ=255) cccAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTc < 1:791688/60‑1 (MQ=255) cccAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTc > 2:791688/1‑60 (MQ=255) aGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTAt > 2:216493/1‑83 (MQ=255) aGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTAt < 1:216493/83‑1 (MQ=255) | TTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAA > NC_000913/3218232‑3218494 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |