Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,299,438	A→G	T155T (ACA→ACG)	yhbO →	stress‑resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,438	0	A	G	100.0%	143.4 / NA	41	T155T (ACA→ACG)	yhbO	stress‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/18);  total (23/18)

GCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACA  >  NC_000913/3299308‑3299572                                                                                                                                   |                                                                                                                                        gcgcCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGa                                                                                                                                 >  1:518463/1‑139 (MQ=255)  cgcCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGAt                                                                                                                                >  2:1222758/1‑139 (MQ=255)      gATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGc                                                                                                                            <  1:1222758/139‑1 (MQ=255)            aTTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGt                                                                                                                      >  1:418357/1‑139 (MQ=255)                 ggggCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa                                                                                                                                    >  2:870000/1‑120 (MQ=255)                 ggggCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa                                                                                                                                    <  1:870000/120‑1 (MQ=255)                                cGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                            <  2:474541/129‑1 (MQ=255)                                cGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                            >  1:474541/1‑129 (MQ=255)                                             aTCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCt                                                                                     >  2:332928/1‑139 (MQ=255)                                               cATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGa                                                                                   >  1:133503/1‑139 (MQ=255)                                                         gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                          >  1:1328466/1‑90 (MQ=255)                                                         gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                          <  2:1328466/90‑1 (MQ=255)                                                         gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGc                                                                         >  1:687390/1‑139 (MQ=255)                                                                   cGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑ttt                                                              >  1:979962/1‑139 (MQ=255)                                                                           gAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCa                                                      <  2:518463/139‑1 (MQ=255)                                                                                ttACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGcc                                                 <  2:687390/139‑1 (MQ=255)                                                                                        aGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCTTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCgtgt                                         >  2:1098191/1‑139 (MQ=255)                                                                                             gTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCg                                    <  1:255017/139‑1 (MQ=255)                                                                                              tCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                            <  2:157311/67‑1 (MQ=255)                                                                                              tCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                            >  1:157311/1‑67 (MQ=255)                                                                                                 tGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                          <  1:149466/50‑1 (MQ=255)                                                                                                 tGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                          >  2:149466/1‑50 (MQ=255)                                                                                                 tGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                            >  1:284995/1‑64 (MQ=255)                                                                                                 tGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                            <  2:284995/64‑1 (MQ=255)                                                                                                      gATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt                                                                                                       <  1:199119/64‑1 (MQ=255)                                                                                                      gATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGt                                                                                                       >  2:199119/1‑64 (MQ=255)                                                                                                        tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                          <  1:807897/43‑1 (MQ=255)                                                                                                        tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                          >  2:807897/1‑43 (MQ=255)                                                                                                           aGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTc                      >  2:1429718/1‑139 (MQ=255)                                                                                                                  cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAg                <  2:286434/139‑1 (MQ=255)                                                                                                                  cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGtgttgt                                      <  2:1406232/116‑1 (MQ=255)                                                                                                                  cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGtgttgt                                      >  1:1406232/1‑116 (MQ=255)                                                                                                                  cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAAtt                             <  2:584300/125‑1 (MQ=255)                                                                                                                  cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAAtt                             >  1:584300/1‑125 (MQ=255)                                                                                                                  cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGc               >  2:656514/1‑139 (MQ=255)                                                                                                                   tGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGcc              <  1:656514/139‑1 (MQ=255)                                                                                                                   tGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTAGATTTCATCGAGcc              <  1:933618/139‑1 (MQ=255)                                                                                                                       tACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCaaa                                                    <  2:206661/97‑1 (MQ=255)                                                                                                                       tACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCaaa                                                    >  1:206661/1‑97 (MQ=255)                                                                                                                          cAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTc       >  2:1337409/1‑139 (MQ=255)                                                                                                                             ccggacgccggacgATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCca    >  2:1408191/1‑139 (MQ=255)                                                                                                                               ggACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCcaca  >  2:755534/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                        GCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCA‑TTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACA  >  NC_000913/3299308‑3299572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑