Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,301,599 G→A G39S (GGC→AGC)  yhbU → U32 peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,301,5990GA100.0% 179.2 / NA 56G39S (GGC→AGC) yhbUU32 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/26);  total (30/26)

GCAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGG  >  NC_000913/3301466‑3301728
                                                                                                                                     |                                                                                                                                 
gcAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCtt                                                                                                                              <  2:155590/139‑1 (MQ=255)
               aaTTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGaaa                                                                                                               >  2:1166869/1‑139 (MQ=255)
                         ctgctCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCgaga                                                                                                                 >  1:1186045/1‑127 (MQ=255)
                         ctgctCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCgaga                                                                                                                 <  2:1186045/127‑1 (MQ=255)
                                    tgccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGt                                                                                          >  2:256619/1‑139 (MQ=255)
                                                cccGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        >  1:778155/1‑129 (MQ=255)
                                                cccGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        <  2:778155/129‑1 (MQ=255)
                                                        ttAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgccg                                                                      <  1:256619/139‑1 (MQ=255)
                                                              cggcCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACt                                                                <  1:1166869/139‑1 (MQ=255)
                                                                 ccATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGt                                                                                     >  1:780034/1‑115 (MQ=255)
                                                                 ccATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGt                                                                                     <  2:780034/115‑1 (MQ=255)
                                                                           cGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        >  2:473175/1‑102 (MQ=255)
                                                                           cGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        <  1:473175/102‑1 (MQ=255)
                                                                              cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcgcg                                                      <  1:1410441/133‑1 (MQ=255)
                                                                              cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcgcg                                                      >  2:1410441/1‑133 (MQ=255)
                                                                              cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAac                                                >  2:828214/1‑139 (MQ=255)
                                                                              cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAac                                                >  2:1069886/1‑139 (MQ=255)
                                                                                 aGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACt                                             <  1:1069886/139‑1 (MQ=255)
                                                                                             tatCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTg                                         <  2:383790/131‑1 (MQ=255)
                                                                                             tatCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTg                                         >  1:383790/1‑131 (MQ=255)
                                                                                              atCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa                                                                                                   <  2:1130704/72‑1 (MQ=255)
                                                                                              atCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGaa                                                                                                   >  1:1130704/1‑72 (MQ=255)
                                                                                              atCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCaa                                                                   >  1:939830/1‑104 (MQ=255)
                                                                                              atCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCaa                                                                   <  2:939830/104‑1 (MQ=255)
                                                                                                   gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtt                                                                                         >  1:1299669/1‑77 (MQ=255)
                                                                                                   gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtt                                                                                         <  2:1299669/77‑1 (MQ=255)
                                                                                                   gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCAt                                    >  1:1526928/1‑130 (MQ=255)
                                                                                                   gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCAt                                    <  2:1526928/130‑1 (MQ=255)
                                                                                                   gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGt                           <  1:828214/139‑1 (MQ=255)
                                                                                                   gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCCTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtttt                                                                                       <  2:928626/79‑1 (MQ=255)
                                                                                                   gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCCTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtttt                                                                                       >  1:928626/1‑79 (MQ=255)
                                                                                                       aaaGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACg                       >  1:259946/1‑139 (MQ=255)
                                                                                                             gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        >  2:164525/1‑68 (MQ=255)
                                                                                                             gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        <  1:164525/68‑1 (MQ=255)
                                                                                                                    aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCgg                                                                                               <  2:624012/54‑1 (MQ=255)
                                                                                                                    aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCgg                                                                                               >  1:624012/1‑54 (MQ=255)
                                                                                                                    aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTa                         >  2:1225932/1‑124 (MQ=255)
                                                                                                                    aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTa                         <  1:1225932/124‑1 (MQ=255)
                                                                                                                     tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGaa                                                                                                                <  1:355473/36‑1 (MQ=255)
                                                                                                                     tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGaa                                                                                                                >  2:355473/1‑36 (MQ=255)
                                                                                                                     tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgccgc                                                                     <  2:526752/79‑1 (MQ=255)
                                                                                                                     tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgccgc                                                                     >  1:526752/1‑79 (MQ=255)
                                                                                                                     tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcgc         >  2:213580/1‑139 (MQ=255)
                                                                                                                     tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcgc         >  2:952782/1‑139 (MQ=255)
                                                                                                                         cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCg     >  2:763958/1‑139 (MQ=255)
                                                                                                                          gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcg                                                        >  1:1267863/1‑87 (MQ=255)
                                                                                                                          gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcg                                                        <  2:1267863/87‑1 (MQ=255)
                                                                                                                          gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTa                         >  2:836966/1‑118 (MQ=255)
                                                                                                                          gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTa                         <  1:836966/118‑1 (MQ=255)
                                                                                                                            cACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTgg  >  1:216167/1‑139 (MQ=255)
                                                                                                                                 cGCCAGCCTTAACTTTATCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCaa                                                                   >  1:959192/1‑69 (MQ=255)
                                                                                                                                 cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        <  2:474070/48‑1 (MQ=255)
                                                                                                                                 cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        >  1:1320443/1‑48 (MQ=255)
                                                                                                                                 cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        <  2:1320443/48‑1 (MQ=255)
                                                                                                                                 cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGttt                                                                                        >  1:474070/1‑48 (MQ=255)
                                                                                                                                 cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCat                                                                   <  2:959192/69‑2 (MQ=255)
                                                                                                                                     |                                                                                                                                 
GCAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGG  >  NC_000913/3301466‑3301728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑