Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,040	C→T	*295* (TAG→TAA)	nlpI ←	lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,040	0	C	T	100.0%	168.9 / NA	53	*295* (TAG→TAA)	nlpI	lipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/27);  total (26/27)

CTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAG  >  NC_000913/3307904‑3308159                                                                                                                                         |                                                                                                                        cTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTa                                                                                                                       >  2:526126/1‑139 (MQ=255)    atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTg                                                                                                                    <  1:540985/139‑1 (MQ=255)      catGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCt                                                                                                                  >  1:1447756/1‑139 (MQ=255)              tGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGAt                                                                                                          >  1:296292/1‑139 (MQ=255)                   aGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGc                                                                                                     <  2:159695/139‑1 (MQ=255)                     cAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCa                                                                                                   >  2:671266/1‑139 (MQ=255)                     cAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCa                                                                                                   >  1:524863/1‑139 (MQ=255)                      aaTTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAg                                                                                                  >  2:1095814/1‑139 (MQ=255)                      aaTTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAg                                                                                                  >  1:521193/1‑139 (MQ=255)                       aTTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAgg                                                                                                 <  1:671266/139‑1 (MQ=255)                                 aaaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGt                                                                                       <  2:404476/139‑1 (MQ=255)                                                cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                        >  1:321138/1‑139 (MQ=255)                                                  cAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                                      <  2:524863/139‑1 (MQ=255)                                                     aGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAAt                                                                   <  2:1447756/139‑1 (MQ=255)                                                         ggTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAgg                                                                            >  2:767091/1‑126 (MQ=255)                                                         ggTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAgg                                                                            <  1:767091/126‑1 (MQ=255)                                                         ggTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCca                                                               >  2:735691/1‑139 (MQ=255)                                                            gtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaaca                                                            <  2:321138/139‑1 (MQ=255)                                                              aTTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAAt                                                          >  1:1341145/1‑139 (MQ=255)                                                               ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                           >  1:47671/1‑105 (MQ=255)                                                               ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                           <  2:47671/105‑1 (MQ=255)                                                                    gAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                     >  1:644565/1‑106 (MQ=255)                                                                    gAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                     <  2:644565/106‑1 (MQ=255)                                                                     aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATaa                                                                    >  1:408645/1‑122 (MQ=255)                                                                     aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATaa                                                                    <  2:408645/122‑1 (MQ=255)                                                                      acaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCg                                                  <  2:1315373/139‑1 (MQ=255)                                                                       caacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                           <  1:398634/97‑1 (MQ=255)                                                                       caacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                           >  2:398634/1‑97 (MQ=255)                                                                          caacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGc                                              >  1:1271157/1‑139 (MQ=255)                                                                             cGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTca                                           >  1:1347915/1‑139 (MQ=255)                                                                             cGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTca                                           >  1:784830/1‑139 (MQ=255)                                                                                ccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaaca                                        <  1:1095814/139‑1 (MQ=255)                                                                                    aCCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGt                                    >  2:1405526/1‑139 (MQ=255)                                                                                                   ggCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTgg                     >  1:627341/1‑139 (MQ=255)                                                                                                        tCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                     >  2:823652/1‑70 (MQ=255)                                                                                                        tCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                     <  1:823652/70‑1 (MQ=255)                                                                                                        tCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaa                                          >  2:655230/1‑113 (MQ=255)                                                                                                        tCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaa                                          <  1:655230/113‑1 (MQ=255)                                                                                                         cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCGACCg               <  2:627341/139‑1 (MQ=255)                                                                                                         cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg               <  1:526126/139‑1 (MQ=255)                                                                                                         cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg               <  1:1405526/139‑1 (MQ=255)                                                                                                         cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg               <  1:735691/139‑1 (MQ=255)                                                                                                         cAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg               <  2:1347915/139‑1 (MQ=255)                                                                                                              aaGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGt                                                                                                >  1:1379643/1‑53 (MQ=255)                                                                                                              aaGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGt                                                                                                <  2:1379643/53‑1 (MQ=255)                                                                                                                   tACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCt                                             >  2:1184554/1‑99 (MQ=255)                                                                                                                   tACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCt                                             <  1:1184554/99‑1 (MQ=255)                                                                                                                      gggCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAg  <  2:784830/139‑1 (MQ=255)                                                                                                                            aTGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa     <  2:1068924/130‑1 (MQ=255)                                                                                                                            aTGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa     >  1:1068924/1‑130 (MQ=255)                                                                                                                                 tACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa     >  2:1210742/1‑125 (MQ=255)                                                                                                                                 tACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa     <  1:1210742/125‑1 (MQ=255)                                                                                                                                      cAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                              <  1:123622/95‑1 (MQ=255)                                                                                                                                         |                                                                                                                        CTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAG  >  NC_000913/3307904‑3308159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑