Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,340,588 T→C L105S (TTA→TCA)  yrbG → putative calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,340,5880TC98.6% 243.3 / ‑3.2 69L105S (TTA→TCA) yrbGputative calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (36/32);  minor base G (1/0);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

TCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAG  >  NC_000913/3340455‑3340721
                                                                                                                                     |                                                                                                                                     
tctcTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCt                                                                                                                                  <  1:1535492/139‑1 (MQ=255)
           acaacGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGttgttg                                                                                                                       <  1:1473787/139‑1 (MQ=255)
           acaacGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGttgttg                                                                                                                       >  2:1473787/1‑139 (MQ=255)
                         ccGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                         >  1:1522293/1‑139 (MQ=255)
                              ggTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    >  1:394392/1‑139 (MQ=255)
                              ggTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    <  2:1522293/139‑1 (MQ=255)
                                tACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTcc                                                                                                  <  1:1077162/139‑1 (MQ=255)
                                 aCAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  1:649786/139‑1 (MQ=255)
                                 aCAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  2:394392/139‑1 (MQ=255)
                                    gCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  1:701710/136‑1 (MQ=255)
                                    gCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 >  2:701710/1‑136 (MQ=255)
                                     cccTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTAct                                                                                             >  2:1274070/1‑139 (MQ=255)
                                     cccTCAGCTCAAACATTATTATTATATTGCGGAGCCTCGGTCTGGCTGCGGTGGTTCGTCCTTTTACCGTCCATTCCGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTAGGCATCGTGGTGGCCGGTTCCGTAct                                                                                             <  2:1003171/139‑1 (MQ=255)
                                                           tatatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGc                                                                       >  1:1261251/1‑139 (MQ=255)
                                                           tatatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCAACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAAGTTAGTCGc                                                                       >  1:912775/1‑139 (MQ=255)
                                                            atatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                         <  2:212755/104‑1 (MQ=255)
                                                            atatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGc                                                                                                         >  1:212755/1‑104 (MQ=255)
                                                                 gCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGa                                                                  >  2:1386386/1‑138 (MQ=255)
                                                                 gCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGa                                                                  <  1:1386386/138‑1 (MQ=255)
                                                                     aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATg                                                                                        >  2:450673/1‑112 (MQ=255)
                                                                     aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATg                                                                                        <  1:450673/112‑1 (MQ=255)
                                                                           ggTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCTGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTtc                                                       >  1:740595/1‑139 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGGCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                              <  2:1077405/78‑1 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCgtg                                                                                                              >  1:1077405/1‑78 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  2:1017995/91‑1 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 >  1:1017995/1‑91 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTa                                                                                               >  2:117814/1‑93 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTa                                                                                               <  1:117814/93‑1 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     <  1:377245/103‑1 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     >  2:377245/1‑103 (MQ=255)
                                                                                 gCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCctct                                                 >  2:1129961/1‑139 (MQ=255)
                                                                                               tCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATGACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTa                                   >  2:1181490/1‑139 (MQ=255)
                                                                                                    ttACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       <  2:881841/66‑1 (MQ=255)
                                                                                                    ttACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       >  1:881841/1‑66 (MQ=255)
                                                                                                    ttACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGa                                                                  <  2:228130/103‑1 (MQ=255)
                                                                                                    ttACCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGa                                                                  >  1:228130/1‑103 (MQ=255)
                                                                                                     tACCGTCCATTCTGATGTTCTTCGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTTTCTTTCTCCTCTTTCTGGCTGTGCTATGgctg                             <  2:912775/139‑1 (MQ=255)
                                                                                                      aCCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTctcct                                                   <  1:1433865/116‑1 (MQ=255)
                                                                                                      aCCGTCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTctcct                                                   >  2:1433865/1‑116 (MQ=255)
                                                                                                          tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     <  2:531601/78‑1 (MQ=255)
                                                                                                          tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     >  1:531601/1‑78 (MQ=255)
                                                                                                          tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGtt                        >  1:767423/1‑139 (MQ=255)
                                                                                                          tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTT‑GTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTtctc                                                     >  1:777368/1‑109 (MQ=255)
                                                                                                          tCCATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTT‑GTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTtctc                                                     <  2:777368/109‑1 (MQ=255)
                                                                                                           ccATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTc                       <  1:1129961/139‑1 (MQ=255)
                                                                                                           ccATTCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTc                       <  2:459697/139‑1 (MQ=255)
                                                                                                              ttCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCGGCTTTCTGGCTGTGCTATGACTGCTGTTCAtt                    >  1:402336/1‑139 (MQ=255)
                                                                                                               tCTGATGTTCTACGCCGTGAATCACCCTTAATGTTTTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTgg                                           <  2:392181/115‑1 (MQ=255)
                                                                                                               tCTGATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTgg                                           >  1:392181/1‑115 (MQ=255)
                                                                                                                  gATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     >  2:1369326/1‑70 (MQ=255)
                                                                                                                  gATGTTCTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACg                                                                                     <  1:1369326/70‑1 (MQ=255)
                                                                                                                        cTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTAt                                  <  2:1460929/115‑1 (MQ=255)
                                                                                                                        cTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTAt                                  >  1:1460929/1‑115 (MQ=255)
                                                                                                                        cTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACtt           >  1:753608/1‑138 (MQ=255)
                                                                                                                        cTACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACtt           <  2:753608/138‑1 (MQ=255)
                                                                                                                         tACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 >  2:1189636/1‑51 (MQ=255)
                                                                                                                         tACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCg                                                                                                 <  1:1189636/51‑1 (MQ=255)
                                                                                                                         tACGCCGTGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGc         >  2:186863/1‑139 (MQ=255)
                                                                                                                         tACGCCGTGAATCACCCTAAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAATTTGc         >  1:1011763/1‑139 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       >  2:1261118/1‑38 (MQ=38)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCg                                                                                                       <  1:1261118/38‑1 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    >  1:1169882/1‑41 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    >  2:1036833/1‑41 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    <  2:1169882/41‑1 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt                                                                                                    <  1:1036833/41‑1 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCa                                                                      >  2:303808/1‑71 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCa                                                                      <  1:303808/71‑1 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAg  >  2:608179/1‑139 (MQ=255)
                                                                                                                                tGAATCACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAg  >  1:277980/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTTAAACTTGCACGTCAG  >  NC_000913/3340455‑3340721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑