Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,675	A→G	V138A (GTC→GCC)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,675	0	A	G	100.0%	227.1 / NA	63	V138A (GTC→GCC)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/28);  total (35/28)

CAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATG  >  NC_000913/3400554‑3400806                                                                                                                          |                                                                                                                                    cAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAgcg                                                                                                                    <  1:1234396/139‑1 (MQ=255)         tAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACggg                                                                                                                            >  1:35754/1‑123 (MQ=255)         tAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACggg                                                                                                                            <  2:35754/123‑1 (MQ=255)              gCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACggg                                                                                                                            >  2:791873/1‑118 (MQ=255)              gCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACggg                                                                                                                            <  1:791873/118‑1 (MQ=255)                  cTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACg                                                                                                   >  1:748636/1‑139 (MQ=255)                      aGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAAtt                                                                                               <  2:748636/139‑1 (MQ=255)                                           aaCCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcg                                                                                            >  1:714027/1‑121 (MQ=255)                                           aaCCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcg                                                                                            <  2:714027/121‑1 (MQ=255)                                            accaccATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTcg                                                                         >  1:652123/1‑139 (MQ=255)                                               accaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACg                                                                                                                              <  1:345398/83‑1 (MQ=255)                                               accaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACg                                                                                                                              >  2:345398/1‑83 (MQ=255)                                                ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGcc                                                                     <  2:652123/139‑1 (MQ=255)                                                ccaTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGcc                                                                     <  2:1380920/139‑1 (MQ=255)                                                 caTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCTCGGATTCACGAATTGcgcg                                                                                          >  2:918394/1‑117 (MQ=255)                                                 caTAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGcgcg                                                                                          <  1:918394/117‑1 (MQ=255)                                                     gAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCg                                                                <  1:464696/139‑1 (MQ=255)                                                       aCCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcgc                                                                                           <  2:1393310/110‑1 (MQ=255)                                                       aCCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcgc                                                                                           >  1:1393310/1‑110 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAgcg                                                                                                                    <  2:1501588/81‑1 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAgcg                                                                                                                    >  1:1501588/1‑81 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGcc                                                                                                                  <  2:682247/83‑1 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGcc                                                                                                                  >  1:682247/1‑83 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGcgcg                                                                                          >  2:503423/1‑107 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGcgcg                                                                                          <  1:503423/107‑1 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGt                                                                                      <  2:1013582/111‑1 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGt                                                                                      >  1:1013582/1‑111 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAAc                                                                                 >  1:313179/1‑116 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAAc                                                                                 <  2:313179/116‑1 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacac                                                          >  2:84314/1‑139 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacac                                                          >  1:533121/1‑139 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacac                                                          >  2:409881/1‑139 (MQ=255)                                                           gTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGcacac                                                          >  2:454743/1‑139 (MQ=255)                                                             cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAc                                                                  >  2:210440/1‑129 (MQ=255)                                                             cGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAc                                                                  <  1:210440/129‑1 (MQ=255)                                                                ttCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCg                                                                <  1:689094/128‑1 (MQ=255)                                                                ttCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCg                                                                >  2:689094/1‑128 (MQ=255)                                                                          ggCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcgcg                                           >  2:780907/1‑139 (MQ=255)                                                                               gCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGcgcg                                                                                                           <  2:1387100/70‑1 (MQ=255)                                                                               gCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGcgcg                                                                                                           >  1:1387100/1‑70 (MQ=255)                                                                               gCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCtt                                      >  1:362015/1‑139 (MQ=255)                                                                               gCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCtt                                      >  1:118732/1‑139 (MQ=255)                                                                                         aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTggg                                                                            >  2:581131/1‑91 (MQ=255)                                                                                         aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTggg                                                                            <  1:581131/91‑1 (MQ=255)                                                                                         aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa                            >  2:1528576/1‑139 (MQ=255)                                                                                         aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa                            <  1:454743/139‑1 (MQ=255)                                                                                         aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa                            >  1:1416807/1‑139 (MQ=255)                                                                                               gCAGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGt                      >  1:223143/1‑139 (MQ=255)                                                                                                 aGCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTg                    <  2:718571/139‑1 (MQ=255)                                                                                                  gCCATCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGc                   >  2:862795/1‑139 (MQ=255)                                                                                                      tCGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCtgtg               <  1:409881/139‑1 (MQ=255)                                                                                                       cGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcg                                                                                            <  2:444221/61‑1 (MQ=255)                                                                                                       cGGTTCTTCAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcg                                                                                            >  1:444221/1‑61 (MQ=255)                                                                                                             ttcAATCAGGAAGGCTTCACGGGCACCAGCTCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGt        >  2:1256928/1‑139 (MQ=255)                                                                                                             ttcAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcgc                                                                                           <  1:1118180/56‑1 (MQ=255)                                                                                                             ttcAATCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTgcgc                                                                                           >  2:1118180/1‑56 (MQ=255)                                                                                                                 aTCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtga    <  1:1528576/139‑1 (MQ=255)                                                                                                                 aTCAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtga    >  2:153539/1‑139 (MQ=255)                                                                                                                   cAGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg  >  2:840582/1‑139 (MQ=255)                                                                                                                    aGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGa                                                                                                         >  2:535161/1‑35 (MQ=255)                                                                                                                    aGGAAGGCTTCACGGGCACCAGCGCCCTGCGCGGa                                                                                                         <  1:535161/35‑1 (MQ=255)                                                                                                                      gAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCAt                              >  1:1428169/1‑108 (MQ=255)                                                                                                                      gAAGGCTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCAt                              <  2:1428169/108‑1 (MQ=255)                                                                                                                          |                                                                                                                                    CAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAACCACCATAGAACCGGTCGCTTCAGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATG  >  NC_000913/3400554‑3400806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑