Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,456,797	G→C	A141P (GCC→CCC)	gspD →	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,456,797	0	G	C	100.0%	280.8 / NA	79	A141P (GCC→CCC)	gspD	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (38/41);  total (38/41)

TTTTCTGAAAGTGGTTCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGGCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATA  >  NC_000913/3456664‑3456919                                                                                                                                        |                                                                                                                           ttttCTGAAAGTGGTTCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGcccccc                                                                                                                       <  1:1046383/139‑1 (MQ=255)   ttCTGAAAGTGGTTCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCt                                                                                                                     >  1:284791/1‑139 (MQ=255)   ttCTGAAAGTGGTTCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCt                                                                                                                     >  1:149234/1‑139 (MQ=255)        aaaGTGGTTCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTcc                                                                                                                <  1:994558/139‑1 (MQ=255)          aGTGGTTCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGc                                                                                                              <  1:1338222/139‑1 (MQ=255)               ttCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTg                                                                                                                    >  1:1108738/1‑128 (MQ=255)               ttCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTg                                                                                                                    <  2:1108738/128‑1 (MQ=255)               ttCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAgat                                                                                                         >  2:6481/1‑139 (MQ=255)                 cGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAgatga                                                                                                       <  2:1302145/139‑1 (MQ=255)                      aGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATgatggat                                                                                                  <  2:512988/139‑1 (MQ=255)                        cTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGc                                                                                                >  2:537462/1‑139 (MQ=255)                            tGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGt                                                                                            >  1:739651/1‑139 (MQ=255)                               aaaaaCATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGc                                                                                         <  1:537462/139‑1 (MQ=255)                               aaaaaCATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGc                                                                                         <  2:1519310/139‑1 (MQ=255)                                     aTCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGt                                                                                   <  2:1111723/139‑1 (MQ=255)                                       cGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTaa                                                                                 >  1:752081/1‑139 (MQ=255)                                         ccAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCggg                                                                                             <  2:1485392/125‑1 (MQ=255)                                         ccAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCggg                                                                                             >  1:1485392/1‑125 (MQ=255)                                         ccAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAAtg                                                                               <  1:457680/139‑1 (MQ=255)                                          cAGGGATGATTGCTGACAGTTCTCGTACAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAAtgt                                                                              <  1:1207639/139‑1 (MQ=255)                                             ggATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAAtgttgt                                                                           <  2:752081/139‑1 (MQ=255)                                               atgatTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTGGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGc                                                                         <  2:1053070/139‑1 (MQ=255)                                                  atTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCAtt                                                                      >  2:836857/1‑139 (MQ=255)                                                    tGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTAt                                                                    >  1:333251/1‑139 (MQ=255)                                                      cTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGa                                                                  >  1:1078862/1‑139 (MQ=255)                                                             ttCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTcc                                                           >  1:1353633/1‑139 (MQ=255)                                                              tctcGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCa                                                          >  2:374374/1‑139 (MQ=255)                                                               ctcGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCaa                                                         <  2:739651/139‑1 (MQ=255)                                                               ctcGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCaa                                                         <  1:836857/139‑1 (MQ=255)                                                                       ggCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGc                                                                                                              <  2:128269/78‑1 (MQ=255)                                                                       ggCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGc                                                                                                              >  1:128269/1‑78 (MQ=255)                                                                       ggCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTa                                                 >  2:409001/1‑139 (MQ=255)                                                                            aGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGa                                                                  >  1:1253761/1‑117 (MQ=255)                                                                            aGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGa                                                                  <  2:1253761/117‑1 (MQ=255)                                                                             ggTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGtt                                                     >  2:668322/1‑129 (MQ=255)                                                                             ggTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGtt                                                     <  1:668322/129‑1 (MQ=255)                                                                                  tgaGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCAtt                                                                      <  1:1530048/107‑1 (MQ=255)                                                                                  tgaGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCAtt                                                                      >  2:1530048/1‑107 (MQ=255)                                                                                  tgaGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGt                                      >  2:1429558/1‑139 (MQ=255)                                                                                  tgaGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGt                                      >  2:790707/1‑139 (MQ=255)                                                                                      ttGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCt                                                                                                                  <  2:685659/59‑1 (MQ=255)                                                                                      ttGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCt                                                                                                                  >  1:685659/1‑59 (MQ=255)                                                                                        ggTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCGGCCAGATGATGGATGCGGGt                                                                                            <  2:1359569/79‑1 (MQ=255)                                                                                        ggTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCGGCCAGATGATGGATGCGGGt                                                                                            >  1:1359569/1‑79 (MQ=255)                                                                                        ggTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGcc                                >  1:155525/1‑139 (MQ=255)                                                                                         gTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCt                               <  1:1274976/139‑1 (MQ=255)                                                                                         gTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCt                               >  1:1350549/1‑139 (MQ=255)                                                                                          tCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTc                              <  1:409001/139‑1 (MQ=255)                                                                                           cACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCTTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTcc                             <  2:1078862/139‑1 (MQ=255)                                                                                           cACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGt                                      <  2:526255/130‑1 (MQ=255)                                                                                           cACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGt                                      >  1:526255/1‑130 (MQ=255)                                                                                             cccGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTccac                           <  1:1429558/139‑1 (MQ=255)                                                                                             cccGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTccac                           <  2:155525/139‑1 (MQ=255)                                                                                              ccGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTg                                                                                                                    >  2:701498/1‑49 (MQ=255)                                                                                              ccGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTg                                                                                                                    <  1:701498/49‑1 (MQ=255)                                                                                              ccGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAAtgtt                                                                             >  2:1195582/1‑88 (MQ=255)                                                                                              ccGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAAtgtt                                                                             <  1:1195582/88‑1 (MQ=255)                                                                                                      tACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATtaataa                  <  1:374374/139‑1 (MQ=255)                                                                                                        ccGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGtt                                                     <  2:581307/102‑1 (MQ=255)                                                                                                        ccGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGtt                                                     >  1:581307/1‑102 (MQ=255)                                                                                                           cTTGAGAATGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCggg                                                                                             >  1:999355/1‑59 (MQ=255)                                                                                                           cTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCggg                                                                                             <  2:999355/59‑1 (MQ=255)                                                                                                           cTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTccac                           >  2:334473/1‑125 (MQ=255)                                                                                                           cTTGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTccac                           <  1:334473/125‑1 (MQ=255)                                                                                                            ttGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGAt            <  1:1172642/139‑1 (MQ=255)                                                                                                             tGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGc                                                                                         >  2:1280338/1‑61 (MQ=255)                                                                                                             tGAGAACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGc                                                                                         <  1:1280338/61‑1 (MQ=255)                                                                                                                gaACGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGaa        >  2:163318/1‑139 (MQ=255)                                                                                                                 aaCGTTCCTGCTCGTGACCTCTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGa         >  2:201156/1‑139 (MQ=255)                                                                                                                   cGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTg          <  2:1460420/134‑1 (MQ=255)                                                                                                                   cGTTCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTg          >  1:1460420/1‑134 (MQ=255)                                                                                                                     ttCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAAtgttgt                                                                           >  1:772990/1‑67 (MQ=255)                                                                                                                     ttCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAAtgttgt                                                                           <  2:772990/67‑1 (MQ=255)                                                                                                                      tCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCaa                                                         >  1:994589/1‑84 (MQ=255)                                                                                                                      tCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCaa                                                         <  2:994589/84‑1 (MQ=255)                                                                                                                      tCCTGCTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATa  <  2:1353633/139‑1 (MQ=255)                                                                                                                           cTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTa                                                                                  >  2:1327365/1‑54 (MQ=255)                                                                                                                           cTCGTGAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTa                                                                                  <  1:1327365/54‑1 (MQ=255)                                                                                                                                gAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCaa                                                         >  2:1125087/1‑74 (MQ=255)                                                                                                                                gAC‑‑CTGCCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCaa                                                         <  1:1125087/74‑1 (MQ=255)                                                                                                                                        |                                                                                                                           TTTTCTGAAAGTGGTTCGCTCAGCTAATGTAAAAACATCGCCAGGGATGATTGCTGACAGTTCTCGTCCAGGCGTAGGTGATGAGTTGGTCACCCGAATTGTACCGCTTGAGAACGTTCCTGCTCGTGAC‑‑CTGGCCCCCCTGCTCCGCCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATA  >  NC_000913/3456664‑3456919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑