Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,503,952	G→A	*262* (TAG→TAA)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,503,952	0	G	A	100.0%	147.5 / NA	47	*262* (TAG→TAA)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

GTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTC  >  NC_000913/3503828‑3504084                                                                                                                             |                                                                                                                                     gTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCg                                                                                                                        >  1:1011319/1‑139 (MQ=255) gTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCg                                                                                                                        <  2:907921/139‑1 (MQ=255)    ccGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGa                                                                                                                     >  2:367290/1‑139 (MQ=255)      ggAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGc                                                                                                                   >  1:768212/1‑139 (MQ=255)                       cGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTg                                                                                                  <  2:526618/139‑1 (MQ=255)                              cTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCATGCCAACGTGCTGTTAc                                                                                           >  1:968378/1‑139 (MQ=255)                               tttGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                          <  1:878481/139‑1 (MQ=255)                                         gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGGCAACGTGCTGTTACTTGCCGGaaaa                                                                                <  2:768212/139‑1 (MQ=255)                                           cTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                              <  1:1073200/139‑1 (MQ=255)                                                gggATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCt                                                                         <  2:319090/139‑1 (MQ=255)                                                   aTGACATTACCGCAGGCGATAGCGTAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCaa                                                                                                      >  2:1039085/1‑107 (MQ=255)                                                   aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTTAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCaa                                                                                                      <  1:1039085/107‑1 (MQ=255)                                                   aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                <  2:1118127/129‑1 (MQ=255)                                                   aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                >  1:1118127/1‑129 (MQ=255)                                                               cAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGc                                                                                                         <  2:848416/92‑1 (MQ=255)                                                               cAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGc                                                                                                         >  1:848416/1‑92 (MQ=255)                                                                 ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCg                                                                                                                        <  2:791727/75‑1 (MQ=255)                                                                 ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCg                                                                                                                        >  1:791727/1‑75 (MQ=255)                                                                 ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                <  2:522655/131‑1 (MQ=255)                                                                 ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                >  1:522655/1‑131 (MQ=255)                                                                 ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                        <  1:367290/139‑1 (MQ=255)                                                                    gATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcattc                                                     <  2:1011319/139‑1 (MQ=255)                                                                       aGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCttt                                                  >  2:1067252/1‑139 (MQ=255)                                                                            aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTAttt                                              <  1:741143/138‑1 (MQ=255)                                                                            aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTAttt                                              >  2:741143/1‑138 (MQ=255)                                                                            aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTc                                             >  1:1321387/1‑139 (MQ=255)                                                                                aaCGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaagta                                                                                                                                   <  2:397457/49‑1 (MQ=255)                                                                                aaCGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaagta                                                                                                                                   >  1:397457/1‑49 (MQ=255)                                                                                    gCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTgg                                                                                                                          >  1:173303/1‑54 (MQ=255)                                                                                    gCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTgg                                                                                                                          <  2:173303/54‑1 (MQ=255)                                                                                        gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                        >  1:349286/1‑116 (MQ=255)                                                                                        gcgcGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                        <  2:349286/116‑1 (MQ=255)                                                                                         cgcgGCGAAAATCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCTGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAtt                                   <  1:939241/136‑1 (MQ=255)                                                                                         cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAtt                                   >  2:939241/1‑136 (MQ=255)                                                                                                          aGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa               <  2:1458671/139‑1 (MQ=255)                                                                                                               cACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACtctc          <  1:759734/139‑1 (MQ=255)                                                                                                                 cGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTA‑TTCAGGGTCGATTATGt                               >  1:39771/1‑115 (MQ=255)                                                                                                                 cGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTA‑TTCAGGGTCGATTATGt                               <  2:39771/115‑1 (MQ=255)                                                                                                                    tGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                              <  2:455578/66‑1 (MQ=255)                                                                                                                    tGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                              >  1:455578/1‑66 (MQ=255)                                                                                                                    tGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                               >  2:642443/1‑113 (MQ=255)                                                                                                                    tGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGt                               <  1:642443/113‑1 (MQ=255)                                                                                                                      ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                >  2:1393193/1‑78 (MQ=255)                                                                                                                      ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                <  1:1393193/78‑1 (MQ=255)                                                                                                                      ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAggg                                         <  2:956986/101‑1 (MQ=255)                                                                                                                      ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAggg                                         >  1:956986/1‑101 (MQ=255)                                                                                                                      ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAct   >  2:747978/1‑139 (MQ=255)                                                                                                                      ccTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAct   >  1:310709/1‑139 (MQ=255)                                                                                                                       cTGGTAAGTATAACGTTGGCGTGAGCATCTTCATGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc  <  2:968378/139‑1 (MQ=255)                                                                                                                          gtaagtaTAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                >  1:127634/1‑74 (MQ=255)                                                                                                                          gtaagtaTAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                <  2:127634/74‑1 (MQ=255)                                                                                                                             |                                                                                                                                     GTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTC  >  NC_000913/3503828‑3504084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑