| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 333,763 | A→G | L224P (CTG→CCG) | yahB ← | putative DNA‑bindng transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 333,763 | 0 | A | G | 96.7% | 201.1 / ‑2.7 | 61 | L224P (CTG→CCG) | yahB | putative DNA‑bindng transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (27/32); total (28/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01 | |||||||||||
CCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGT > NC_000913/333629‑333895 | ccTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAAt < 1:478897/139‑1 (MQ=255)ccTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAAt < 1:556783/139‑1 (MQ=255) ttATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGcc < 1:195828/139‑1 (MQ=255) tGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTgcagca > 1:420978/1‑139 (MQ=255) tGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTgcagca > 2:916294/1‑139 (MQ=255) tCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAAc > 2:1119456/1‑139 (MQ=255) cGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCATCGGAATTGACTCCTGCCCGTGCAGCAAcc < 2:1065026/139‑1 (MQ=255) gTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGc < 2:432050/139‑1 (MQ=255) gTAACCAGCAGCGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGc < 1:993634/139‑1 (MQ=255) aCCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCa > 2:622580/1‑139 (MQ=255) agcagGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACtt > 1:88440/1‑139 (MQ=255) tGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTaa > 2:1096358/1‑139 (MQ=255) aTCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTCCAGCAACCAGCCCACTTTTTTA‑TTAATCGTAt > 2:1514355/1‑139 (MQ=255) tCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATg > 2:795940/1‑139 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGcgcg < 1:622580/139‑1 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGcgcg < 1:1119456/139‑1 (MQ=255) cTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGcgcg < 1:1096358/139‑1 (MQ=255) cACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGc < 2:1433751/95‑1 (MQ=255) cACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGc > 1:1433751/1‑95 (MQ=255) aCGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGgtg < 2:420978/139‑1 (MQ=255) ggACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCCGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGtc < 1:1514355/139‑1 (MQ=255) aCCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGtcct < 1:702351/139‑1 (MQ=255) ccATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGtcctc < 1:795940/139‑1 (MQ=255) ccATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGtcctc < 2:88440/139‑1 (MQ=255) ccATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGtcctc < 1:916294/139‑1 (MQ=255) cATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATgcg > 2:751153/1‑128 (MQ=255) cATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATgcg < 1:751153/128‑1 (MQ=255) gTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGcc > 2:1470813/1‑102 (MQ=255) gTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGcc < 1:1470813/102‑1 (MQ=255) gTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTATTTAATCGTATGCGCGGTGTCCTccac > 1:1295551/1‑139 (MQ=255) aaTCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTATTTAATCGTATGCGCGGTGTCCTccacca < 2:1295551/139‑1 (MQ=255) aTCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACttttt < 2:962598/107‑1 (MQ=255) aTCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACctttt > 1:962598/1‑107 (MQ=255) ggCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTtta < 1:839345/105‑1 (MQ=255) ggCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTtta > 2:839345/1‑105 (MQ=255) ggCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAatat < 2:579740/139‑1 (MQ=255) cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTcc < 1:847972/73‑1 (MQ=255) cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTcc > 2:847972/1‑73 (MQ=255) cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGtcctc < 1:278053/126‑1 (MQ=255) cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGtcctc > 2:278053/1‑126 (MQ=255) cAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCCGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTa > 1:295070/1‑139 (MQ=255) aaaaaCCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAgg > 1:574953/1‑139 (MQ=255) gCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGcc < 1:1063385/63‑1 (MQ=255) gCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGcc > 2:1063385/1‑63 (MQ=255) gCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTAt > 2:152620/1‑83 (MQ=255) gCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTAt < 1:152620/83‑1 (MQ=255) gCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATg > 1:281274/1‑139 (MQ=255) gCACTGACATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATg > 1:750892/1‑139 (MQ=255) aCATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTccacc > 2:386067/1‑94 (MQ=255) aCATTTGGTGTTGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTccacc < 1:386067/94‑1 (MQ=255) ttGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCa > 1:1391053/1‑49 (MQ=255) ttGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCa < 2:1391053/49‑1 (MQ=255) ttGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATgcg < 2:1439320/70‑1 (MQ=255) ttGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATgcg > 1:1439320/1‑70 (MQ=255) ttGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGcgcg > 2:326349/1‑72 (MQ=255) ttGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGcgcg < 1:326349/72‑1 (MQ=255) ttGAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAgg > 2:653850/1‑139 (MQ=255) gAAATCTGGCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCt < 2:574953/139‑1 (MQ=255) ggCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGt < 2:295070/139‑1 (MQ=255) gCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAgaga > 1:1454504/1‑92 (MQ=255) gCACCGGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAgaga < 2:1454504/92‑1 (MQ=255) | CCTGGCGCGGATTATGGATTTGTCGGGTAACCAGCAGGGATTGCGTCATCGCCTCACGGACCATGTAATCCGGCAAAAAACCAATTCCTTCACCTAAAATCTGGCACTGACATTTGGTGTTGAAATCTGGCACCAGAATTGACTCCTGCCCGTGCAGCAACCAGCCCACTTTTTTA‑TTAATCGTATGCGCGGTGTCCTCCACCATAATATTAGGGTAGAGACGCAGTTGGCTTTCGGCGATGGGTTCCGGCACAAAGGCTAACGGGT > NC_000913/333629‑333895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |